Thermo Scientific Shake 'n Stack 6244, Operating And Maintenance Manual

Page: 12 / 92

Shake ‘n Stack 2-3 Thermo Scientific
Section 2
Unpacking and Installation
For ease of use and reproducibility when hybridising in bottles, the
temperature display is calibrated to show internal bottle temperature as
opposed to oven air temperature. When the shaking platform is used,
allowance must be made for the difference between the temperature shown
on the display and the temperature achieved within a liquid-filled vessel on
the shaker.
This difference can be calculated by filling the protocol specific vessel with
enough distilled water to cover an immersible thermometer, and setting
the oven to the desired product temperature. After enough time for the
temperature to have stabilized, record the thermometer reading and
subtract this value from the setpoint value. The remainder must be added
to the setpoint value for execution of the actual protocol. For instance, a
setpoint value of 55°C minus a thermometer reading of 58°C yields a
remainder of -3°C which means that the adjusted setpoint value for this
protocol will be 52°C. Repeat this process to verify that the thermometer
reading is /-1°C of the setpoint value. This difference should be
determined for each oven and/or shaker protocol.
The lowest target temperature which the oven can achieve is 8°C above
ambient temperature. Avoid placing the unit in direct sunlight, or in any
area where it is likely to be exposed to hazardous or volatile chemicals.
When positioning the Hybridisation Oven, ensure a gap of at least 2 in.
(5 cm) is maintained between the sides of the oven and any other
equipment or object.
The oven may be located in a fume cabinet or similar chamber, provided
the cabinet is not used to contain hazardous or volatile chemicals with low
flash points.
Full details of Technical Specifications are given in Section 8.
Note
If the Shake ‘n Stack Oven is not used as specified in this manual,
the protection provided by the equipment may be impaired.
Set the Required
Temp (continued)