Thermo Scientific Shake 'n Stack 6244, Operating And Maintenance Manual

Page: 67 / 92

© Thermo
Scientific
, May 2003. Issue 7 14
Figure 3: Inserting Membranes in a Bottle
1. Shallow tray containing 2 x SSPE (SSC).
Mesh.
Membrane.
Roll up mesh & membrane.
2. Place 10-15mls of SSPE (SSC) into
a bottle and then insert the roll centrally.
3. Secure cap and, holding bottle
horizontally, roll to catch the trailing
edge of the mesh,
continue rolling in
the same direction until coil of mesh
and membrane is well positioned.
4.
Pour out SSPE (SSC) and replace with
pre-warmed prehybridisation buffer.
5. Place the bottle in the Oven so that it
rotates in the Oven in the same
direction, as it was unrolled in step 3.