Thermo Scientific Multidrop Combi nL, User Manual

Page: 28 / 106

Routine Operation
Reagent reservoir
28 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific
There are different possibilities for holding the reagent to be dispensed.
The liquid to be dispensed can be kept directly in the reagent reservoir
of the pressure/vacuum system (Figure 4–17). There is a white reagent
filter of 43 µm at the bottom of the 250 ml reagent reservoir that filters
the solution to be dispensed. Nevertheless, all reagents still have to be
filtered before use.
Figure 4–17.
Reagent reservoir connected
A 1000 ml reagent reservoir (Cat. no. N07490) with a longer tubing is
also available for larger volumes often used in automated systems.
Warning
If other reagent reservoirs than recommended by Thermo
Fisher Scientific are used, we are not liable for any damages arising out
of their use. Never connect glass bottles if they are not intended for
high pressure/vacuum usage.
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In nanodispensing, however, there might be a shortage of reagent. A
separate reagent vessel or tube filled with the liquid to be dispensed can
be inserted into the reagent reservoir. Standard laboratory test tubes,
50 ml and 15 ml, can be placed inside the reagent reservoir as such, but
you will need a tube holder to keep smaller test tubes upright. You can
remove the reagent filter if you use separate small reagent tubes. The
solution is filtered beforehand with a suitable filter.
The 43 µm white reagent filter (Cat. no. N07493) can be replaced by
pulling the filter away from the end of the tube (Figure 4–18). In some
maintenance situations, for example, when cleaning a leaking or clogged
dispensing valve, it is easier to remove both the filter and the connective
tube from the cap of the reagent reservoir. Refer to “How to clean a
leaking dispensing valve” on page 73.
Reagent
reservoir
Reagent reservoir
Pressure/vacuum system