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Routine Operation

Instrument options

Thermo Fisher Scientific

Thermo Scientific Multidrop Combi nL User Manual

There are two types of computer interfaces available: RS-232 and USB.
Go to the

Options

menu. Select the

Computer interface

row.

Press

Use the

and

Down

arrow keys to select the computer interface in

use.

Press

Computer interface

This selection also has to be

done when you use the

instrument through FILLit

Software. Refer to Chapter

2: “

Installing FILLit

Software

” in the

FILLit

Software for Multidrop

Combi nL User Manual

(Cat. no. N17562).

Summary of Contents for Multidrop Combi nL

Page 1: ...Thermo Scientific Multidrop Combi nL User Manual Rev 1 2 Cat no N07171 ...

Page 2: ...technological developments This manual is subject to change without prior notice as part of continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions ...

Page 3: ...manual also describes all the features and specifications of the Multidrop Combi nL instrument as well as ordering information Read the manual in its entirety before operating the instrument Keep the user manual for future reference The user manual is an important part of the instrument and should be readily available For PC software related issues refer to the Thermo Scientific FILLit Software fo...

Page 4: ... of hazards as indicated The following symbols and markings appear on the type label and the instrument itself Power ON s Power OFF s Serial number s Catalog number s Date of manufacture s Consult instructions for use s WEEE symbol This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE Directive 2012 19 EU s Safety symbols and markings Safety symbol...

Page 5: ... Warning Risk of electric shock s Warning Biohazard risk s Warning Risk of injury to the user s s Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application s Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest s Warning and other markings used in the documentation ...

Page 6: ...er laboratory safety precautions such as wearing protective clothing and following approved laboratory safety procedures l Use of the Multidrop Combi nL in ways other than those described in the documentation supplied with the equipment may result in injury to persons or damage to the property Avoid unintended use of the equipment for example using incompatible materials making unauthorized modifi...

Page 7: ...uum system 13 Dispensing applications 13 Calibrations 14 Advantages of using Multidrop Combi nL 14 Functional Description 15 Instrument layout 15 Front view 15 Back view 16 Installation 17 Delivery check 17 Checking delivery 17 Unpacking 17 Requirements 18 Precautions and limitations 18 Installation setups 18 Releasing the transport lock 19 Installing the priming vessel 20 Installing the pressure ...

Page 8: ...volume 47 Dispensing direction 48 Running a protocol 50 Save and edit protocol 51 Select protocol 54 Delete protocol 54 Instrument options 56 Device information 56 Computer interface 57 Startup protocol 58 Instrument settings 59 Calibration 60 Liquid factor standalone calibration 60 Accuracy check 61 Factor calculation 63 Screen saver 65 Shutdown 65 Emergency situations 66 Maintenance 67 Maintenan...

Page 9: ... to refit the transport lock 82 Packing for service 83 Service contracts 84 Disposal of the instrument 85 Technical Specifications 86 General specifications 86 Safety specifications 87 Live parts 87 In conformity with the requirements 87 Remote control to Multidrop Combi nL 88 Frequently Asked Questions 89 Q As 89 Troubleshooting Guide 91 Error and warning codes 91 Hazards 93 Electrical 93 Mechani...

Page 10: ...uum dispensing system 26 Figure 4 16 Eight dispensing valves inserted into the manifold 26 Figure 4 17 Reagent reservoir connected 28 Figure 4 18 Removing the reagent filter 29 Figure 4 19 Keyboard of the Multidrop Combi nL 29 Figure 4 20 Main view on the display of the Multidrop Combi nL 30 Figure 4 21 Example of dispensing with selected wells 42 Figure 4 22 Calibration curve vs factor and volume...

Page 11: ... main view 32 Table 4 3 Default parameter values for different plate types 35 Table 4 4 Relationship between dispensing speed and pressure 44 Table 4 5 Speed settings vs distance movement and frequency 45 Table 5 6 Maintenance checklist 67 Table 6 7 General specifications 86 Table 8 8 Error messages reported 91 Table 9 9 Instrument catalog number 95 Table 9 10 Codes for accessories 95 ...

Page 12: ...apid and continuous dispensing of liquids into 96 384 and 1536 well plates It can dispense 50 nl into the entire 384 well plate in 6 seconds or 50 nl into the entire 1536 well plate in 21 seconds The Multidrop Combi is lightweight transportable and compact on a laboratory bench Figure 1 1 Multidrop Combi nL microplate dispenser The instrument model available is l 5840400 Multidrop Combi nL 100 240...

Page 13: ...h of reagents Empty function Reliable low volume dispensing can be carried out with the Multidrop Combi nL With a volume range of 50 nl to 50 µl for 96 384 and 1536 well plates with dispensing heights from 5 to 55 mm the Multidrop Combi nL offers outstanding flexibility for a wide range of applications Multidrop Combi nL can be used for example in cell and bead based assays and to a greater extent...

Page 14: ...ations are created calculated and transferred with FILLit Software For more information refer to the FILLit Software for Multidrop Combi nL User Manual Cat no N17562 The Multidrop Combi nL microplate dispenser has several operational advantages l Flexible low volume range of 50 nl 50 µl l Flexible dispensing height of 5 55 mm l High speed dispensing of column row and well specific volumes l Superi...

Page 15: ...and back views of the Multidrop Combi nL instrument The front view of the Multidrop Combi nL instrument is shown in Figure 2 2 Figure 2 2 Multidrop Combi nL front view Instrument layout Front view Display Protective cover Dispensing valve head Plate carrier Priming vessel Keyboard Reagent reservoir Dispensing valve 8 Clamp Light indicator ...

Page 16: ... is shown in Figure 2 3 Figure 2 3 Close up of the Multidrop Combi nL keyboard and display The back view of the Multidrop Combi nL instrument is shown in Figure 2 4 Figure 2 4 Multidrop Combi nL back view Back view Display Keyboard Mains switch Serial RS 232C connector USB 1 1 2 0 compatible Mains connector Instrument label Pressure vacuum system Pressure vacuum luer connector ...

Page 17: ...lacement If any parts are damaged contact your local Thermo Fisher Scientific representative Move the packed instrument to its site of operation To prevent condensation the instrument should be left in its protective antistatic plastic wrapping until the ambient temperature has been reached Unpack the Multidrop Combi nL instrument and accessories carefully with the arrows on the transport package ...

Page 18: ...duce operating noise at a level that would be harmful No sound level measurements are required after installation Place the instrument on a normal laboratory bench The net weight of the entire equipment is approx 9 6 kg 21 2 lbs The instrument operates at voltages of 100 240 Vac and a frequency range of 50 60 Hz l Always ensure that the local supply voltage in the laboratory conforms to that speci...

Page 19: ...is released before operating the instrument Figure 3 5 Transport lock and transport lock tag present 1 Unscrew the two screws marked 1 and 2 of the transport lock with the hexagonal screwdriver supplied Figure 3 6 so that the track mechanism is loosened Figure 3 6 Removing the transport lock 2 Remove the screws the transport lock piece and the transport lock tag from the screw marked 1 Figure 3 6 ...

Page 20: ...rument Figure 3 7 Transport lock storage To install the priming vessel 1 Insert the blue priming vessel Cat no N07271 into its slot on the left of the plate carrier by pressing it over a snap lock Figure 3 8 Figure 3 8 Inserting the priming vessel 2 Insert a priming vessel tube assembly or cap Cat no N05843 into the drain of the priming vessel or a vessel underneath the drain To install the pressu...

Page 21: ...re 3 9 The red luer plugs avoid particles from entering the pressure vacuum system unnecessarily Figure 3 9 Removing the red luer plugs 2 Connect the luer connector of the hosing to the pressure vacuum system box Figure 3 10 Connect the other end of the hosing through a similar luer connector to the reagent reservoir Figure 3 11 for producing pressure and vacuum Figure 3 10 Connecting the hosing t...

Page 22: ...sembly Figure 3 12 A 4 Fit the reagent reservoir hosing on top of the bottle to the manifold Figure 3 12 B of the dispensing valves It is enough to insert the hosing only halfway onto the manifold tube connector Finally insert the hosing into the groove intended for it Figure 3 13 Figure 3 12 Close up of the manifold Caution To detach the manifold tube connector pull the hosing off Do not twist th...

Page 23: ...ne power outlet which has a protective conductor also called earth or ground Switch the instrument on using the mains switch Figure 2 4 Warning Interruption of the protective conductor inside or outside the instrument or disconnection of the protective conductor terminal may make the instrument dangerous s l If the instrument starts properly l The display lights up l The plate carrier moves to the...

Page 24: ...ssure has been reached Caution Unfastening the tubing while the pressure is on can cause a hazardous situation flood or spillage l Check that there is always enough liquid in the reagent reservoir and ensure that the filter is below the liquid level l Check after each dispensing that the priming vessel is not filled up if the tube drain is not used s 5 Press PRIME l The lifting mechanism is lowere...

Page 25: ... 6 Press START l The dispensing protocol selected with the user interface is started l The clamp Figure 3 14 tightens the microplate against the lower left corner of the plate carrier If the protocol or predispense is not started follow the instructions on the display or check Chapter 9 Troubleshooting Guide ...

Page 26: ...he Empty function The principle of the pressure system is shown below Figure 4 15 Figure 4 15 Principle of the pressure vacuum dispensing system The eight microsolenoid dispensing valves are inserted into the dispensing valve head The modular design is shown below Figure 4 16 Figure 4 16 Eight dispensing valves inserted into the manifold Dispensing valves Manifold Dispensing valve 8 Groove for hos...

Page 27: ...r pressure Calibrations are created calculated and transferred with the Multidrop Combi nL PC software Calibration is a combination of a liquid to be dispensed and a speed Calibration will ensure accurate dispensing with the instrument A calibration contains a series of five calibration points of which each consists of a volume time pair Each calibration point has a fixed valve open time The appro...

Page 28: ...ir use Never connect glass bottles if they are not intended for high pressure vacuum usage s In nanodispensing however there might be a shortage of reagent A separate reagent vessel or tube filled with the liquid to be dispensed can be inserted into the reagent reservoir Standard laboratory test tubes 50 ml and 15 ml can be placed inside the reagent reservoir as such but you will need a tube holde...

Page 29: ... buttons are described in detail below The arrow keys are used to select modify and change the parameters and to navigate in the display The OK button is used to accept the selection The START button is used to start the dispensing protocol The STOP button is used to stop the protocol at any time The plate and dispensing valve head are returned to the home position While editing values the changes...

Page 30: ...d by pressing the STOP button The PRESSURE button is used to raise the pressure at startup Once the pressure has been reached pressing the PRESSURE button again releases the pressure Therefore you can toggle with the PRESSURE button However raising the pressure is started by pressing the PRESSURE button and stopped only by pressing the STOP button if the pressure has not yet been reached Note Push...

Page 31: ...Table 4 2 on page 32 The info text bar shows explanatory information on how to proceed and which keys to use This section visualizes navigation in the Multidrop Combi nL user interface The main view changes according to the selections you make either with the Up or Down arrow keys or the OK button The available buttons and their function are shown on the info text bar The color of the items for ex...

Page 32: ...spensing volume and liquid on page 36 Column Row selection on page 37 Save and edit protocol on page 51 Dispensing speed on page 45 Shaking on page 44 Dispensing offsets on page 45 Predispensing volume on page 47 Dispensing direction on page 48 Device information on page 56 Computer interface on page 57 Startup protocol on page 58 Instrument settings on page 59 Calibration on page 60 ...

Page 33: ...oir and the reagent Close the protective cover 3 Make sure you are in the Main menu 4 Select the plate type and insert the plate 5 Select the dispensing volume and the liquid 6 Press the PRESSURE button and wait till the pressure has risen Only at startup 7 Keep the PRIME button down until the tubings are completely filled 8 Press the START button to start the protocol The instrument dispenses the...

Page 34: ...nstrument to another Calibrations can also be transferred to the internal software Use FILLit Software to enter the valve correction factors while changing the spare valve For more information refer to the FILLit Software for Multidrop Combi nL User Manual Cat no N17562 This section describes procedures related to the Multidrop Combi nL internal software This section describes the relevant dispens...

Page 35: ... 500 16 96 well low DW deep well 22 mm 600 23 384 well low volume 7 5 mm 25 8 5 384 well low profile 10 mm 70 11 384 well standard 15 mm 130 16 384 well DW 22 mm 200 23 1536 well low volume 5 mm 2 6 1536 well standard 10 5 mm 13 11 5 You can dispense without warning to the maximum dispensing volume but once the maximum is exceeded a warning appears and you have to press OK to continue dispensing T...

Page 36: ... down the arrow key continuously Then select Liquid Calibration using the Left and Right arrow keys The different liquids set are l Water l 1 BSA bovine serum albumin l 30 glycerol l DMSO dimethyl sulfoxide l Methanol If you have a different liquid than the five liquids mentioned above you can calibrate the new liquid with FILLit Software or use the liquid factor to calibrate the set volume The op...

Page 37: ...utton You can dispense either into the whole plate or only into certain selected columns or rows Go to the Main menu Select the Select columns and rows row Press OK If you want to dispense into all the wells of the plate select Full plate if it is not already shown on the screen If you want to dispense only into some of the wells select Select columns or Select rows using the Down arrow key Column...

Page 38: ... OK button to toggle between a filled or empty column Use the Down arrow key and select Accept and press OK after selecting all the columns you wish You can press the STOP button to exit the column selection without making any changes The dispensing volume selected in the Volume and Liquid window see Dispensing volume and liquid on page 36 is shown in the bottom left box You can adjust the dispens...

Page 39: ...is now activated bright red Adjust the dispensing volume of the column where the cursor is located using the Up and Down arrow keys Press OK after selecting the desired volume Use the Down arrow key and select Accept and press OK after selecting the column volumes you wish You can press the STOP button to exit the volume selection without making any changes Volume value box ...

Page 40: ... Use the OK button to toggle between a filled or empty row Use the Down arrow key and select Accept and press OK after selecting all the rows you wish You can press the STOP button to exit the row selection without making any changes The dispensing volume selected in the Volume and Liquid window see Dispensing volume and liquid on page 36 is shown in the bottom left box You can adjust the dispensi...

Page 41: ...e STOP button to exit the volume selection without making any changes You can first select columns of a microplate with a certain volume and then select rows of the same microplate with another volume or vice versa Where the selected columns and rows cross each other the wells will be filled with the row or column volume that was last selected when such a protocol is executed Refer to the example ...

Page 42: ...e plate type that is selected in the Main menu Note The protocol settings do not normally have to be changed in routine use s Note An exclamation mark will appear in the menu tab and in the circle on the main view row in question when the setting differs from the default setting s The changes made to the protocol settings only remain in the instrument memory if the protocol is saved with a name se...

Page 43: ...g 1 BSA E g Water Use the Up and Down arrow keys to select the speed The available and calibrated speeds are colored black On the other hand the unavailable and uncalibrated speeds are colored white The available range is 1 to 5 To use unavailable speeds the liquid first has to be calibrated using the unavailable speed Press OK The optimal dispensing speeds for different liquids are factory set Fo...

Page 44: ...ed Pressure Speed 1 3 psi Speed 2 4 psi Speed 3 5 psi Speed 4 6 psi Speed 5 7 psi The Shake function allows you to shake the plate immediately after dispensing it without any additional selection steps By default the shaking is turned OFF Go to the Settings menu Select the Shaking row Press OK Use the Up and Down arrow keys to select the duration of the shake You can set the time in increments of ...

Page 45: ... distance movement and frequency Speed Movement distance mm Frequency Hz Low 2 10 Medium 1 15 High 1 20 Press OK This main view row includes both the dispensing height and the dispensing offset functions Go to the Settings menu Select the Offsets row Press OK The track mechanism and the dispensing valve head move to the setting position When you adjust the height the dispensing valve head moves ne...

Page 46: ...row keys to set the dispensing height While you change the offset value the dispensing valve lifting mechanism moves accordingly The height is set in 0 05 mm increments in both the up and down directions The minimum height value depends on the selected plate type see Table 4 3 The default dispensing height is 1 mm above the selected plate Note You can adjust the dispensing height of low profile pl...

Page 47: ...oves accordingly The offset is set in 0 05 mm increments in both the and directions The maximum offset value depends on the selected plate type Press OK If the OK button is not pressed within 60 seconds the adjustments are disregarded and the main view returns to the Settings menu y axis the dispensing valve head moves x axis the plate carrier moves You can change the predispensing volume the volu...

Page 48: ...0 to 200 nl in 50 nl increments Note that the predispensing value can be zero if predispensing is not required Press OK The row wise dispensing direction being the fastest option is default for each plate type However you may change the dispensing direction if necessary Go to the Settings menu Select the Dispensing direction row Press OK Dispensing direction Column wise direction Row wise directio...

Page 49: ...well plate selection the columns with 16 wells are filled in two phases first every other row A C E G O starting from the columns selected and then the dispensing valve head carrier shifts sideways so that the remaining rows B D F H P are filled in the opposite order If column wise dispensing is used it shifts the dispensing valve head carrier first sideways filling 16 wells and then the plate car...

Page 50: ...y liquid to avoid overheating of the pump l Check that there is always enough liquid in the reagent reservoir and ensure that the reagent filter is below the liquid level Use a test tube placed inside the reagent reservoir if a limited amount of liquid is available l Check after each dispensing that the priming vessel is not filled up if the tube drain is not used s 1 Make sure you are in the Main...

Page 51: ...fic Multidrop Combi nL User Manual 51 Go to the Main menu Select the Select and Save Protocol row Press OK Press the Right arrow key to name save the name of your protocol Enter the name of your protocol Use the arrow keys to choose the character you want Save and edit protocol ...

Page 52: ...er than the others whereby some of the names can display less than 20 characters in the protocol list shown as three dots at the displayed name The protocol name is automatically capitalized s To remove characters use the Backspace button Use the Down arrow key and if needed the Left and Right arrow keys to select the Backspace button and then the OK button to remove the character s Use the Down a...

Page 53: ... User Manual 53 The sandglass window will appear while the Save action is in progress The protocol name is now shown on the Select and Save Protocol row You can create a maximum of 100 protocols including the factory defined protocols When you save the protocol all the following parameters are saved ...

Page 54: ...ve Protocol row Press OK Select the protocol using the Up and Down arrow keys Press OK to accept the selection The protocol is now selected Note In the protocol list only the protocols made for the plate type in question are shown s To delete an existing protocol for a particular plate type first select the plate type see Plate type on page 34 and then select the Select and Save Protocol row Selec...

Page 55: ...to enter the edit mode To delete the protocol use the Delete button Use the Down arrow key and if needed the Left and Right arrow keys to select the Delete button and then the OK button to delete the protocol You cannot delete default protocols the Delete button is not activated Also if you have selected a protocol to be a startup protocol it cannot be deleted The protocol is now deleted Delete bu...

Page 56: ...hese parameters are set in the Options menu The values shown in the Options menu remain in the instrument memory and are instrument specific not protocol specific Note You do not normally have to change the instrument options in routine use s The device information shows the name of the instrument the internal software version and the serial number of the instrument unit Go to the Options menu Pre...

Page 57: ...nd USB Go to the Options menu Select the Computer interface row Press OK Use the Up and Down arrow keys to select the computer interface in use Press OK Computer interface This selection also has to be done when you use the instrument through FILLit Software Refer to Chapter 2 Installing FILLit Software in the FILLit Software for Multidrop Combi nL User Manual Cat no N17562 ...

Page 58: ...n menu when the Multidrop Combi nL is powered on Go to the Options menu Select the Startup protocol row Press OK All protocols stored in the instrument memory are displayed Use the Up and Down arrow keys to select the startup protocol The protocols are listed grouped and indented under each plate type Press OK The protocol name is appended with the plate type Startup protocol ...

Page 59: ...type and the protocol are those just selected Note that the sandglass window always appears while the Save action is in progress in the Options menu You can choose whether or not the instrument produces a sound for different functions Note that all functions make a certain sound Go to the Options menu Select the Instrument settings Buzzer row Press OK Instrument settings ...

Page 60: ... and liquid on page 36 Liquid factor is a step where dispensing accuracy can be verified and corrected at one selected volume Factor is a correction factor which can be used for example after speed pressure or liquid change where there is no calibration curve for a new speed or a new liquid First dispensing of multiple dispenses into a microplate strip is carried out according to the volume select...

Page 61: ...lculate the liquid factor Refer to Factor calculation on page 63 Use the Up and Down arrow keys to select the factor for the liquid used The default settings for the liquid the dispensing speed and the dispensing volume are presented in the Factor window Use the Left arrow key to check the dispensing volume Use the Right arrow key to open the Quick Guidance window Press OK To perform an accuracy c...

Page 62: ...e weight of the empty strip to zero so that the net mass of the dispensed liquid can be obtained from the balance without any calculations s l Place the plate onto the plate carrier of the Multidrop Combi nL l Press START on the keyboard to start dispensing or STOP to escape at the liquid speed and volume set in Main Volume and Liquid The liquid is dispensed into the empty strip a default number o...

Page 63: ...may be omitted in the accuracy check but with liquids having a different density to water the mass conversion to real volumes should also be carried out in the accuracy check step s Example Accuracy check with water at 200 nl volume set 1 Measured liquid mass 0 1815 g 2 Conversion to volume nanoliters 1000000 0 1815 0 9969 water density at 22 C 182064 nl 3 ACCURACY 100 182064 nl 179200 Expected vo...

Page 64: ...factor In this case the instrument dispenses 0 984 times liquid compared to the value in the calibration curve 3 Save the protocol Note The factor affects the whole calibration curve throughout the volume range and the volumes are changed respectively in the same proportion as the check volume Figure 4 22 The Liquid factor is however protocol sensitive and must be saved with the protocol The liqui...

Page 65: ...ftware for Multidrop Combi nL User Manual Cat no N17562 The Multidrop Combi nL screen saver shown below appears when the instrument has not been used for 20 minutes You can close the screen saver and return to the previous display by pressing any key To shut down the Multidrop Combi nL Warning Remove any microplates still on the instrument Dispose of all microplates and strips as biohazardous wast...

Page 66: ...nt into the OFF position The pressure is released Note To pause operation and hold the instrument on standby just push the protective cover in to release the pressure s 5 Wipe the instrument surfaces with a soft cloth or tissue paper moistened with deionized distilled water a mild detergent e g dishwashing liquid or soap solution 6 If you have spilled infectious agents on the dispenser disinfect w...

Page 67: ...ith a mild sterilizing solution See Regular and preventive maintenance on page 68 Clean the case of the instrument and the dispensing area periodically See Regular and preventive maintenance on page 68 Clean the tubings when necessary See How to clean the tubings on page 69 Clean the reagent reservoir when necessary See How to clean the reagent reservoir on page 78 Clean the plate carrier when nec...

Page 68: ...t e g dishwashing liquid or 70 ethanol when necessary Immediately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage Abrasive cleaning agents are not recommended because they are likely to damage the plastic instrument cover Caution Always switch the instrument off and disconnect the power cable before cleaning the dispenser s Caution The ...

Page 69: ...proper tubing operation 1 Filter all reagents detergents solutions and water before use to remove solid particles 2 Rinse the tubings with distilled and filtered laboratory grade water always after using the instrument Warning Before a prolonged standby run the tubings dry to avoid microbial growth from developing s If there is reason to suspect that particles have entered the tubings clean the tu...

Page 70: ...ternational Products Corporation or equivalent under the dispensing valves so that the liquid is in contact with the valves Figure 5 24 Figure 5 24 Cleaning the tubings using a reagent basin l Press the EMPTY button for several minutes l Place another disposable reagent basin or a 96 well plate filled with distilled and filtered laboratory grade water under the dispensing valves so that the liquid...

Page 71: ...ontainer for example a decanter 4 Add water or detergent for example 1 Micro 90 into the reagent reservoir 5 Press the PRIME button to raise the pressure This action leads to fluid dripping into the waste container from the valve tube ends 6 When you have rinsed enough release the pressure by pressing the PRESSURE button or by pushing the protective cover in 7 Repeat Steps 3 to 5 with the fluids y...

Page 72: ...nt through the liquid path To obtain optimal performance and a maximum useful life for the dispensing valves it is important that the recommended cleaning maintenance instructions are followed If the dispensing valve is leaking or clogged refer to How to clean a leaking dispensing valve on page 73 and or How to clean a clogged dispensing valve on page 75 Routine maintenance of the dispensing valve...

Page 73: ...TY button is also used for emptying the remaining liquid from the tubings and dispensing valves backflush option Periodic maintenance constitutes for example cleaning a leaking or clogged dispensing valve replacing a dispensing valve or replacing a tubing set and a manifold The procedures are all explained in detail below Foreign particles can cause leakage of a dispensing valve tip Figure 4 16 an...

Page 74: ...er disposable reagent basin now filled with distilled and filtered laboratory grade water under the dispensing valves so that the liquid is in contact with the valves cf Figure 5 24 l Press the EMPTY button for several minutes 3 Clean the reagent reservoir 4 Replace the 43 µm reagent filter and the tube into the cap of the reagent reservoir 5 Add distilled and filtered laboratory grade water into ...

Page 75: ...Step 1 above If the dispensing valve is still clogged after the cleaning attempts replace the dispensing valve For more information refer to Replacing a dispensing valve on page 75 If the cause for the dispensing valve change is clogging of the valve first clean the tubings and the reagent reservoir For more information refer to How to clean the tubings on page 69 and How to clean the reagent rese...

Page 76: ...rop Combi nL User Manual Thermo Fisher Scientific Figure 5 28 Unfastening the dispensing valve 4 Pull the dispensing valve downwards Figure 5 29 Figure 5 29 Pulling the dispensing valve downwards 5 Replace the dispensing valve with a new one Figure 5 30 Hexagonal screwdriver ...

Page 77: ...o set the appropriate value in the valve correction menu Open FILLit Software and select Instrument Valve correction Enter the given value in the respective valve channel position and press Update to save the value See the details of FILLit Software in the FILLit Software for Multidrop Combi nL User Manual Cat no N17562 provided with the instrument Note Check that your instrument has the embedded ...

Page 78: ...spensing valve tube ends into a waste container for example a decanter 5 Prime the tubing with distilled and filtered laboratory grade water 6 Reconnect the flexible dispensing valve tube ends to the dispensing valves Figure 5 26 and press the hosing into the groove Figure 5 31 Figure 5 31 Replacing the tubing set and manifold 7 Prime the tubing to ensure the proper functioning of the dispensing v...

Page 79: ...and if you spill liquids on it during use To clean the plate carrier follow the instructions below Caution Clean the plate carrier surface if needed using a soft cloth or tissue paper soaked in a mild detergent solution e g dishwashing liquid soap solution or 70 ethanol Wipe up spills immediately Do not use formaldehyde or strong alkaline solutions s If you have spilled infectious agents on the pl...

Page 80: ...sal of biohazardous or radioactive waste Refer to local regulations for the disposal of infectious material Warning The samples can be potentially infectious Dispose of all used plates strips disposable gloves syringes disposable tips and so on as biohazardous waste s If you have spilled infectious agents carry out the decontamination procedure Warning The decontamination procedure should be perfo...

Page 81: ...resulting in bad test results s Warning The decontamination procedure should be performed by authorized trained personnel wearing disposable gloves protective glasses and clothing in a well ventilated room s 1 Prepare the decontaminant for example 1 3 Virkon solution or 200 ml 4 glutaraldehyde solution or another agent recommended by your safety officer 2 Empty the reagent reservoir Ensure that yo...

Page 82: ...can be autoclaved All the tubings can be autoclaved in the same bag with the reagent reservoir 14 After performing this decontamination procedure enclose a signed and dated Certificate of Decontamination both inside the transport package and attached to the outside of the package see Appendix A Certificate of Decontamination 1 Remove the red transport lock from the back panel of the instrument Fig...

Page 83: ...esented below Caution It is important that the instrument is thoroughly decontaminated before it is removed from the laboratory or any servicing is performed on it s Prior to shipping the instrument for service remember to specify the fault when you are in touch with your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department Packing for service ...

Page 84: ... foam absorbers of the instrument and lower the dispenser into the carton Refer to the packing instructions l Place the accessories box into the carton and fit this between the foam absorbers on top of the instrument l Inform about the use of hazardous materials Enclose a dated and signed Certificate of Decontamination see Appendix A Certificate of Decontamination both inside and attached to the o...

Page 85: ...dures for biohazardous or radioactive waste disposal Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The procedures vary by country Pollution degree 2 see Safety specifications on page 87 Method of disposal Electronic waste Contaminated waste Infectious waste Regarding the original packaging and packi...

Page 86: ... conditions 40 C to 70 C packed in transport packaging Storage conditions 25 C to 50 C packed in transport packaging Autoclaving conditions of the priming vessel and reagent reservoir Pressure 1 bar Temperature 121 C Time 20 min Control panel Keys arrow keys OK START STOP PRIME EMPTY SHAKE PRESSURE TIP WASH Interface Serial RS 232C USB 1 1 2 0 compatible Plate types 96 384 and 1536 well plates Dis...

Page 87: ...rs the following markings Type 838 100 240 Vac 50 60 Hz 100 VA CE cCSAus monogram Multidrop Combi nL conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 Subpart B Class B July 2004 2012 19 EU WEEE Directive 2006 42 EC Machinery Directive The safety specifications are also met under the following environmental c...

Page 88: ...on degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation s The Multidrop Combi nL can be used as part of an automated system When the Multidrop Combi nL is in remote mode the main view is as shown below For...

Page 89: ...er all reagents detergents solutions and water before use to remove any solid particles present Q What materials come into contact with liquids to be dispensed A The standard Multidrop Combi nL materials that come into contact with liquids to be dispensed are l Reagent reservoir polypropylene PP l Reagent filter stainless steel AISI 316 and polytetrafluoroethylene PTFE Teflon l Tubings chemical re...

Page 90: ...u see the Multidrop Combi nL internal software version number A When the instrument is powered on the version number appears on the top of the screen during the startup animation Device information under the Options menu also provides the internal serial number Q What is the orifice of the dispensing valve A The orifice is 100 µm Q Do you need to recalibrate the instrument when a dispensing valve ...

Page 91: ...vice Plate Y position error Plate carrier cannot move Power off on or contact service Z position error Dispensing valve lifting mechanism cannot move Power off on or contact service No more memory for storing user data Too many protocols saved Delete protocols that are not used Not primed Predispensing undone Press the PRIME button Missing prime vessel Priming vessel missing Insert the priming ves...

Page 92: ...drop Combi nL User Manual Thermo Fisher Scientific Press OK to acknowledge the error and then press PRIME Press OK to acknowledge the error and then insert the priming vessel Press OK to acknowledge the error and then pull the protective cover over the dispensing valve head ...

Page 93: ...ning message is shown below Press OK to acknowledge the warning and then reselect the dispensing volume This instrument is designed to provide full user protection When correctly installed operated and maintained the instrument will present no hazards to the user The following recommendations are given to ascertain added user safety Ensure that the mains supply cable supplied with the unit is alwa...

Page 94: ...ess the STOP button on the control panel Infectious samples and corrosive fluids are commonly used with this equipment The hands off nature of the system allows the user to dispense into the reaction wells without getting into direct contact with these fluids However the wells that have been in contact with potentially hazardous fluids must be handled with utmost care Always wear hand and eye prot...

Page 95: ...x N07497 Reagent reservoir 250 ml Nalgene propylene w o tube connectors N07489 Reagent reservoir 250 ml polypropylene accessory assembly incl reagent reservoir reagent filter tubings and manifold N07490 Reagent reservoir 1000 ml polypropylene accessory assembly incl reagent reservoir reagent filter tubings and manifold N09504 Glass reagent reservoir 250 ml with accessory assembly incl reagent rese...

Page 96: ...ist of accessories 96 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Code Item N04001 Cord USB 2 0 A B 1 8 2 0 m 0300033 Tool 3mm DIN911 Allen key Handled N07523 Tool 1 5mm DIN911 Allen key Zn Cont ...

Page 97: ...en decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological Radioactive Specific information about contaminants Decontamination procedure1 Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by ...

Page 98: ...Certificate of Decontamination 98 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific ...

Page 99: ...ay fluid valve for high precision and high speed dispensing of liquids EIA Enzyme immunoassay empty To draw back to the liquid bottle all the liquid in the hoses and valves EN European Norm EU European Union home position The plate carrier is positioned to the furthest right of the transfer rails IEC International Electrotechnical Commission liquid factor Standalone calibration of the liquid used ...

Page 100: ...Glossary 100 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific ...

Page 101: ...olumn selection 38 41 column row selection column selection 38 full plate 37 row selection 32 37 40 selected wells 41 computer interface 32 57 88 control panel 29 86 94 current protocol 99 D dead volume 86 decontamination 66 68 79 80 81 82 84 85 97 99 procedure 80 default 35 36 37 42 43 44 45 46 47 48 55 61 62 64 protocols 55 values 35 64 deleting a protocol 54 91 density 43 63 99 device informati...

Page 102: ...13 14 24 26 27 28 30 33 36 38 40 43 50 60 61 62 63 64 65 66 68 70 72 73 74 79 82 89 99 factor 10 14 36 60 61 63 64 65 99 factor calculation 60 61 63 luer connector 21 M maintenance 3 6 28 30 66 67 68 69 72 73 84 87 manifold 10 22 26 68 69 73 77 78 84 89 95 99 transport plug assembly 22 84 markings 99 N naming a protocol 32 42 51 nanodispensing 28 navigating 29 31 O opening an existing protocol 54 ...

Page 103: ...ns 37 38 41 selecting columns and rows 41 selecting rows 32 37 40 41 selecting wells 41 serial cable 95 serial number 4 56 84 90 service 66 67 68 81 83 84 91 95 contracts 68 84 setting the column volume 38 setting the row volume 40 shaking 32 44 shutdown 65 68 specifications 3 84 85 86 87 speed 14 27 36 43 44 45 60 62 65 89 99 startup 30 32 33 50 55 58 90 protocol 32 55 58 stopping the protocol 29...

Page 104: ...104 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific ...

Page 105: ...Thermo Fisher Scientific Thermo Scientific Multidrop Combi nL User Manual 105 Notes ...

Page 106: ...106 Thermo Scientific Multidrop Combi nL User Manual Thermo Fisher Scientific Notes ...

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