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Copyright

Copyright



2011 Thermo Fisher Scientific Corporation. All rights reserved. Reproduction of the

accompanying user documentation in whole or in part is prohibited.

Patents

This product is protected by the following patents:
US 6447729, Method and means for magnetic particle specific binding assay, and US 6448092, Separation
device for microparticles involving a magnetic rod.
KingFisher Duo also has national and international patents pending.

Trademarks

“BindIt” is a trademark and “KingFisher” and “Microtiter” are registered trademarks of Thermo Fisher
Scientific Inc. and its subsidiaries.
All other trademarks and registered trademarks are the property of their respective holders.

Disclaimer

Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate
technological developments. This manual is subject to change without prior notice as part of a continuous
product development. Although this manual has been prepared with every precaution to ensure accuracy,
Thermo Fisher Scientific assumes no liability for any errors or omissions, nor for any damages resulting from
the application or use of this information. This manual supersedes all previous editions.

Remarks on screenshots

The version number displayed in screenshots may not always be the one of the currently released version.
Screenshots are only replaced if the content related to the application has changed.

No liability for consequential damages

Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to
use this product.

Power failure

The system requires uninterrupted power supply in order to operate correctly. Thermo Fisher Scientific has no
responsibility whatsoever for system malfunctions arising from power failures.

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Summary of Contents for KingFisher Duo

Page 1: ...Thermo Scientific KingFisher Duo User Manual Rev 1 0 ...

Page 2: ......

Page 3: ...Thermo Scientific KingFisher Duo User Manual Rev 1 0 Cat no N12420 ...

Page 4: ...orporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previou...

Page 5: ...manual also describes all the features and specifications of the KingFisher Duo instrument as well as ordering information Note that simulated data appears in the screen captures Read the manual in its entirety before operating the instrument Keep the user manual for future reference The user manual is an important part of the instrument and should be readily available during use of the instrument...

Page 6: ...arkings appear on the type label and the instrument itself Power ON Power OFF Warning Hot surface risk of burns Warning Risk of body parts hair jewelry or clothing getting caught in a moving part Serial number Catalog number Date of manufacture Consult instructions for use WEEE symbol This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE Directive ...

Page 7: ...ual Warning Risk of electric shock Warning Biohazard risk Warning Risk of injury to the user s Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest Warning and other markings used in the documentation ...

Page 8: ...specifically designed for the device Never open any other covers of the KingFisher Duo than the front lid or the maintenance door Figure 2 3 while the instrument is plugged into a power source Never force a microplate onto the instrument The KingFisher Duo is intended for laboratory research use only Observe proper laboratory safety precautions such as wearing protective clothing and following app...

Page 9: ...gnetic particle processing 15 Working with a magnetic rod 16 Collecting magnetic particles 16 Releasing magnetic particles 16 Washing magnetic particles and incubation 16 Changing the volume during the magnetic particle processing 16 Magnet heads 17 Heating blocks 17 Elution blocks 18 Shield plate 18 USB port for PC 18 USB ports for memory devices 18 Consumables 18 Reagents 19 Installation 21 What...

Page 10: ...ser protocols 38 Selecting the protocol 38 Run menu 39 Instrument options 43 Export device report 43 Export run log 44 Import protocol 45 Export protocol 46 Maintenance protocol 47 Information 47 Language 48 Buzzer 49 Date and time 49 Handling the consumables 51 Tip combs 51 Plates 52 Elution strip 53 How to start 53 Shutdown 56 Emergency situations 56 Maintenance 57 Regular and preventive mainten...

Page 11: ...cations 64 Safety specifications 64 In conformity with the requirements 64 Troubleshooting Guide 67 Troubleshooting guide 67 Ordering Information 69 KingFisher Duo 69 List of accessories and consumables 69 List of spare parts 70 List of KingFisher Kits 70 Certificate of Decontamination 71 System Log 73 Chapter 6 Chapter 7 Chapter 8 Appendix A Appendix B ...

Page 12: ...Contents 10 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific ...

Page 13: ...processor Figure 1 1 is designed for automated transfer and processing of magnetic particles in microplate format The patented technology of the KingFisher Duo system is based on the use of magnetic rods covered with a disposable specially designed tip comb and plates The instrument functions without any dispensing or aspiration parts or devices Samples and reagents including magnetic particles ar...

Page 14: ...Introduction to the KingFisher Duo Principle of operation 12 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific ...

Page 15: ...ngFisher Duo front view with front lid and maintenance door open Figure 2 3 KingFisher Duo front view with plates and front lid and maintenance door closed Instrument layout Front view Interchangeable heating block Display Keypad Shield plate blue dashed line Front lid open Turntable Interchangeable KingFisher Duo head USB port for memory stick Front lid closed Elution block fixed On Off switch Ma...

Page 16: ...he plastic tip comb For more details refer also to Magnet heads on page 17 Heating blocks on page 17 and Elution blocks on page 18 Up to two plates can be simultaneously on the turntable and processed automatically one after another The processing of two sequential runs is started with the 1st plate with samples in a 96 well plate Then you prepare the 2nd sample plate while the first plate is stil...

Page 17: ...into the corresponding plate position according to the protocol instructions During the operation the front lid can be closed or open Figure 2 3 The closed door protects the processing against environmental contamination The operating principle employed is MPP inverse magnetic particle processing technology Figure 2 7 Rather than moving the liquids the magnetic particles are moved row wise on the ...

Page 18: ...c rods together with the tip comb are lowered into the wells and the magnetic rods are lifted off Magnetic particles are released by moving the tip comb up and down several times at considerably high speed until all the particles have been mixed with the substance in the next reaction Washing the magnetic particles is a frequent and an important processing phase Washing is a combination of the rel...

Page 19: ...KingFisher Duo elution strip 12 pin magnet head 1 12 samples per run 8 processing positions including the elution strip Up to 1 ml processing volume The 6 pin magnet head is used with a corresponding plastic 6 tip comb and a Thermo Scientific KingFisher Flex 24 deep well plate 6 pin magnet head 1 6 samples per run 3 processing positions optionally with two plates up to 7 positions Up to 5 ml proce...

Page 20: ...n top of the USB port for memory stick on the back panel see Figure 2 4 A USB cable Cat no N04001 is supplied with the instrument There are two parallel USB ports one on the back panel and one on the front panel Figure 2 4 and Figure 2 3 designed for Protocol import export Export of the run log file for traceability The user can either use the USB port on the back panel or the front to accept an e...

Page 21: ...s such as PCR polymerase chain reaction restriction analysis and in several enzymatic reactions The KingFisher Kits are listed below KingFisher Blood DNA Kit Cat no 97010196 KingFisher Cell and Tissue DNA Kit Cat no 97030196 KingFisher Plant DNA Kit Cat no 97050196 KingFisher Total RNA Kit Cat no 97020196 KingFisher Viral NA Kit Cat no 97040196 For more details refer to List of KingFisher Kits on ...

Page 22: ...Functional Description Reagents 20 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific ...

Page 23: ...t with the instrument and are immediately available when you open the package Thermo Scientific KingFisher Duo Brief User s Guide BindIt Software installation CD KingFisher Duo User Manual BindIt Software for KingFisher Instruments User Manual Thermo Scientific User Awareness of Symbols Declaration of Conformity document Caution Do not touch or loosen any screws or parts other than those specifica...

Page 24: ...area is flat dry clean and vibration proof and leaves additional room for accessories cables and reagent bottles There is at least 10 cm on the laboratory bench of free space around the instrument for ventilation The ambient air is clean and free of corrosive vapors smoke and dust The ambient temperature range is between 5 C 41 F and 40 C 104 F The humidity is low so that condensation does not occ...

Page 25: ...when handling KingFisher Duo heads Warning This product contains very strong permanent magnets People wearing a pacemaker or metallic prostheses should not use this product A pacemaker or prostheses may be affected or damaged if it comes in close contact with a strong magnetic field This section describes the installation setups that you must carry out before operating or relocating the instrument...

Page 26: ...re that the mains switch Figure 2 4 on the front panel is in the OFF position Never operate your instrument from a power outlet that has no ground connection Never use a power supply cable other than the Thermo Scientific power supply cable designed for your region 1 Connect the power supply cable to the power supply connector and plug in the instrument Figure 3 11 2 Connect the power supply to a ...

Page 27: ...re that the correct magnet head is attached If the check is all right proceed with your own runs This section describes the installation setups of the interchangeable KingFisher Duo heating blocks and magnet heads When necessary the factory set 12 well heating blocks can be changed to the 6 well heating blocks To change the heating blocks 1 Take away the heating block by lifting it 2 Insert the fi...

Page 28: ... Microtiter deep well 96 plate 50 1000 μl KingFisher Duo heating block for 96 DW plate KingFisher Duo elution strip 30 130 μl KingFisher Duo heating block for elution strip fixed recommended filling volume Warning The heating block and or the elution block surface can be hot whereby there can be risk of burns Caution The heating block is specifically designed for the plates listed below to ensure ...

Page 29: ...rument or any metal surfaces Keep the KingFisher Duo heads always in their respective plastic boxes when not in use Figure 3 13 It is very important to keep the KingFisher Duo heads away from each other and other magnets at all times Clashing of the magnets together may cause serious damage to the magnets Table 3 2 Magnet head vs tip comb Magnet head Description Tip comb Description KingFisher Duo...

Page 30: ...s product contains very strong permanent magnets People wearing a pacemaker or metallic prostheses should not use this product A pacemaker or prostheses may be affected or damaged if it comes in close contact with a strong magnetic field The factory set 12 pin magnet head can be changed to the 6 pin magnet head Fastening changing the magnet head is controlled by the software To change the magnet h...

Page 31: ...the magnet rods against the tip comb holder frame Figure 3 15 Removing the two finger screws on top of the magnet head 5 Insert the other magnet head by slotting it into the tip comb holder and by refitting the two finger screws on top of the magnet head Figure 3 16 and Figure 3 17 6 The tip moves on top of the shield plate at the end of the changing process 7 Screw the maintenance door on again b...

Page 32: ...ice To refit the transport lock 1 To fit the transport lock choose the Transportation_lock protocol under the Maintenance menu by using the Up and Down arrow keys 2 Then press the START key 3 Switch off the power 4 Refit the transport lock into place by screwing the finger screw with spring suspension clockwise Figure 3 18 There is only one possible position for the finger screw to lock once the p...

Page 33: ...tation Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual 31 Figure 3 18 Refitting the transport lock 5 Figure 3 19 shows the transport lock refitted Figure 3 19 Transport lock refitted Caution Ensure that the power is switched off ...

Page 34: ...Installation Packing the instrument for transportation 32 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific ...

Page 35: ...und connection This section describes the KingFisher Duo control panel and internal software The keypad is shown in Figure 4 20 Figure 4 20 Keypad of the KingFisher Duo The relevant keys and control buttons are described in detail below To select the protocol and to navigate on the display To accept the selection To confirm a performed step in the protocol for example plate loading or removal Swit...

Page 36: ...display of the KingFisher Duo There are three menus in the KingFisher Duo user interface Protocols Run and Maintenance In routine use you mainly navigate in the Protocols Run menu You can navigate between the menus using the Left and Right arrow keys The clock on the menu bar shows real time The factory protocols are arranged into folders according to the type of analyses The protocols tab row is ...

Page 37: ...isualizes navigation in the KingFisher Duo user interface The main view changes according to the selections you make either with the Up or Down arrow keys or the OK button To move into the submenus use the OK button The available buttons and their function are shown on the info text bar The color of the items for example the icon and main view row in the main view changes when they are selected ac...

Page 38: ...Routine Operation Control panel 36 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific The main views of each menu tab are shown below Protocols Run Maintenance ...

Page 39: ...cting the protocol on page 38 DNA Factory DNA RNA User RNA Factory Protein Cells User Other Last used protocol s Run menu on page 39 Run Log Run Instrument options on page 43 Export device report on page 43 Export run log on page 44 Import protocol on page 45 Export protocol on page 46 Maintenance protocol on page 47 Information on page 47 Language on page 48 Buzzer on page 49 Date and time on pag...

Page 40: ...l software This section describes the factory user set protocols They are found in the Protocols menu Factory protocols are protocols which are created by Thermo Fisher Scientific and preloaded into the instrument internal memory in the factory e g Thermo Scientific KingFisher Kit protocols or downloaded from the KingFisher website www thermoscientific com kingfisher User protocols are protocols w...

Page 41: ...s the Left arrow key to return In the Run menu the Run Log view is shown when there is no ongoing run Once a protocol run is on the Run view is shown Go to the Run Log menu Select the protocol of which you want to see the run log and press OK to see the details The time shown refers to when this protocol was last started Used plates and strips and when the protocol was last run are also shown Run ...

Page 42: ...u you can see the progress of the selected protocol as shown below Refer to Selecting the protocol on page 38 Press the STOP button if you want to terminate the run You are asked to confirm the termination or to continue Note that whenever a user pauses the operation by pressing the PAUSE button during a run the Pause icon flashes on the menu bar after the Pause message appears on the display ...

Page 43: ...e and so on See more below Press OK to continue During the run the remaining run time is shown in smaller numericals on the time bar The time shown in bold and larger numericals in the middle of the window indicates the time remaining until the following pause During the pause the user must be prepared for example to load the plate dispense or unload the plate and elution strip Time left for the n...

Page 44: ...gin of the window you can see how the steps bar progresses Once the protocol is completed the Run Log view appears see view below When a protocol is started in the Protocol menu a small Run icon is superimposed on the protocol symbol of the active protocol The views of a one protocol and a two protocol run are shown below ...

Page 45: ...Once the USB memory device is plugged in a small memory stick icon appears at bottom right of the window You can export the instrument report A USB memory device has to be plugged in to the instrument Figure 4 22 B Figure 4 22 USB memory device unplugged A and plugged in B The KingFisher Duo device report includes the serial number software versions motor offsets and accelerations temperature cont...

Page 46: ...og A USB memory device has to be plugged in to the instrument Figure 4 22 B The KingFisher Duo run log includes the protocol name when the protocol was started and stopped and the plates and strips used of the all the user and factory protocol runs as well as those of all the UV protocol and maintenance protocol runs Go to the Maintenance menu Select the Export Run Log row and press OK Export run ...

Page 47: ...t arrow key to cancel You can import a protocol for example a BindIt protocol file bdz from BindIt Software or another instrument A USB memory device has to be plugged in to the instrument Figure 4 22 B Go to the Maintenance menu Select the Import Protocol row and press OK Attach the USB memory device or press the Left arrow key to cancel Import protocol ...

Page 48: ...ocol file bdz to BindIt Software or another instrument A USB memory device has to be plugged in to the instrument Figure 4 22 B Go to the Maintenance menu Select the Export Protocol row and press OK Use the Up and Down arrow keys to select the protocol group Press OK to continue Attach the USB memory device or press the Left arrow key to cancel Export protocol ...

Page 49: ...ng block or run the operational check protocol Go to the Maintenance menu Select the Maintenance protocol row and press OK All available maintenance protocols will be displayed Use the Up and Down arrow keys to select the maintenance protocol Press START to run the desired protocol Press the Left arrow key to return The device information shows the name of the instrument the internal software vers...

Page 50: ...KingFisher Duo User Manual Thermo Fisher Scientific Use the Up and Down arrow keys to select Press the Left arrow key to return You can set the language of the internal software Go to the Maintenance menu Select the Language selection row and press OK Language ...

Page 51: ...ey to return You can choose whether or not the instrument produces a sound for different functions Note that all functions make a certain sound Go to the Maintenance menu Select the Buzzer on off row and press OK Use the Up and Down arrow keys to select whether the buzzer audible sound is on or off The default is Buzzer on Press OK to accept the selection Press the Left arrow key to return You can...

Page 52: ...keys to select the date the time the date format or the time format Press OK to set for example the date format Use the Up and Down arrow keys to select the desired date format The available date formats are dd mm yyyy dd mm yyyy dd mm yyyy mm dd yyyy and yyyy mm dd The default is dd mm yyyy Press the Left arrow key to cancel or return ...

Page 53: ...o 12 tip combs correctly 1 Take an individual KingFisher Duo 12 tip comb from the package Figure 4 23 Figure 4 23 KingFisher Duo 12 tip comb packed in a separate bag 2 Unpack the plastic bag and place one of the tip combs onto the tip row default row B of the KingFisher 96 deep well plate Figure 4 24 Avoid bending the tip combs to ensure proper instrument operation Figure 4 24 Placing the KingFish...

Page 54: ...w default row B of an empty KingFisher Flex 24 deep well plate Figure 4 26 Figure 4 26 KingFisher Duo 6 tip comb inserted 3 Before you use the tip comb press the tip comb evenly by hand against the enclosed 24 deep well plate The KingFisher Duo is only compatible with two deep well plates KingFisher Flex 24 deep well plates and Microtiter deep well 96 plates Figure 4 27 and Table 4 4 Figure 4 27 K...

Page 55: ... magnet head Figure 4 28 Figure 4 28 KingFisher Duo elution strips separate and in a frame The elution strip is typically used to perform the elution step that concentrates the purified sample It is easy to pipette small volumes into the elution strip due to its low design The eight elution strips are also convenient for storage and can be collected onto the 96 elution frame Figure 4 28 To start t...

Page 56: ...e The two plate positions and the A1 positions of the two plate positions are clearly marked on the turntable When the instrument is in its basic position plate position 1 is under the KingFisher Duo head Note that at the end of a protocol run the turntable may stop in a different position than the starting position Loading the plate and elution strip is shown below Figure 4 30 and Figure 4 31 Mak...

Page 57: ...om the plate Figure 4 32 Figure 4 32 Tip comb in the tip comb holder 5 When the turntable rotates the magnet head moves on top of the fixed shield plate that forms a protective cover 6 Close the front lid The front lid protects the instrument against environmental contamination Note The front lid can be left open if desired This action does not break the run Elution strip Tip comb in the tip comb ...

Page 58: ... the turntable surface and the adjacent instrument surface with a soft cloth or tissue paper moistened with distilled water a mild detergent SDS sodium dodecyl sulfate or soap solution 3 If you have spilled infectious agents on the turntable disinfect it with 70 ethanol or another disinfectant see Decontamination procedure on page 58 If an abnormal situation occurs during operation such as fluids ...

Page 59: ... alkaline solutions from outer surfaces to prevent damage Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose the surfaces to concentrated acids or concentrated alcohols for prolonged periods of time as they may cause damage Clean the display areas with a mild laboratory detergent The keypad has a wipe c...

Page 60: ...to break the magnets while cleaning Warning This product contains very strong permanent magnets People wearing a pacemaker or metallic prostheses should not use this product A pacemaker or prostheses may be affected or damaged if it comes in close contact with a strong magnetic field If required wipe the shield plate using a soft cloth or tissue paper soaked in a mild detergent solution SDS soap s...

Page 61: ...e decontamination procedure should be performed by authorized trained personnel wearing disposable gloves protective glasses and clothing in a well ventilated room 1 Wear disposable gloves to protect yourself 2 Prepare the decontaminant 200 ml 4 glutaraldehyde solution or another agent recommended by your safety officer 3 Empty the turntable 4 Switch OFF the power and disconnect the mains supply c...

Page 62: ...f hazardous materials Decontaminate the instrument beforehand Install the transport lock Place the KingFisher Duo head into its transport package Pack the instrument according to the enclosed packing instructions Use the original packaging to ensure that no damage will occur to the instrument during shipping Any damage will incur additional labor charges Enclose a dated and signed Certificate of D...

Page 63: ...arning Decontaminate the instrument before disposal Refer to Decontamination procedure on page 58 Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Warning The used lithium Li battery is regulated waste and must be disposed of according to local regulations The Li battery has to be changed by an authorized service technician only Instructions for chan...

Page 64: ...Maintenance Disposal of the instrument 62 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific ...

Page 65: ...kage ca 16 kg 35 lbs ca 21 kg 46 lbs Operating conditions indoor use 5 C to 40 C maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Indoor use only Transportation conditions 40 C to 70 C packed in transport packaging Storage conditions 25 C to 50 C packed in transport packaging Mains power supply 100 240 Vac 50 60 Hz nominal Automatic volta...

Page 66: ...sher Duo heads Plates per deck 2 Plate types disposable Recommended filling volume Microtiter deep well 96 plate 50 1000 μl KingFisher Flex 24 deep well plate 200 5000 μL Elution strip Special design 1 x 12 format Tip combs polypropylene disposable 12 6 in one frame for Microtiter deep well 96 plate or KingFisher Flex 24 deep well plate Automatic tip load Yes Heating cooling temperature plate row ...

Page 67: ...to 50 relative humidity at 40 C Mains supply fluctuations 10 from nominal Installation category overvoltage category II according to IEC 60664 1 see Note 1 Pollution degree 2 according to IEC 60664 1 see Note 2 Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electri...

Page 68: ...Technical Specifications In conformity with the requirements 66 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific ...

Page 69: ...ated contact service 6 The turntable rotating mechanism is out of position Switch the instrument OFF and ON and try again If the error appears during initialization or is otherwise repeated contact service 7 The magnet head holder lifting mechanism is out of position Switch the instrument OFF and ON and try again If the error appears during initialization or is otherwise repeated contact service 8...

Page 70: ...ice If the error still appears contact service 214 Export failed Try another USB memory device If the error still appears contact service 215 Attached USB memory is not supported Possible NTFS formatted USB memory Change memory or reformat to FAT 220 235 261 270 271 285 File system error Contact authorized technical service 250 251 Corrupt transfer file Contact authorized technical service 280 284...

Page 71: ...odes for accessories and consumables Code Item Quantity 5189010 BindIt Software package 1 97003500 KingFisher Duo 12 tip comb for Microtiter deep well 96 plate 50 pcs 97003510 KingFisher Duo 6 tip combs and KingFisher Flex 24 deep well plate 12 pcs of 24 DW plates each including 4 tip combs 48 pcs 97003520 KingFisher Duo elution strip 40 pcs 97003530 KingFisher Duo Combi pack for Microtiter deep w...

Page 72: ...2 KingFisher Duo heating block for 24 DW plate 1 2305290 Serial cable F9 F25 for RS 232C port 1 N04001 USB A B device cable 1 8 m 1 Longer USB cables available from PC stores The following optimized DNA RNA purification kits for the KingFisher Duo are available Table 8 11 Table 8 11 Codes for purification kits Code Item Package size 97010196 KingFisher Blood DNA Kit 1 x 96 97030196 KingFisher Cell...

Page 73: ...contaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological Radioactive Specific information about contaminants Decontamination procedure1 Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the u...

Page 74: ...Certificate of Decontamination 72 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific ...

Page 75: ...Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual 73 Appendix B System Log Instrument name number User Date Comments PHOTOCOPIABLE ...

Page 76: ...System Log 74 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific ...

Page 77: ...articles enable the purification of a variety of target molecules magnetic rod The rods which are magnetic and collect magnetic particles Figure 4 32 The rods do not collect the particles on their own the magnetic rods must always be protected by a tip comb plate The disposable plates 1 2 where all the reagents and samples are located and where the processing takes place Two different types of 24 ...

Page 78: ...Glossary 76 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific ...

Page 79: ...7 58 64 disposal of instrument 61 disposal of materials 58 DNA 19 37 38 70 DNA RNA 37 70 E elution 14 17 18 26 41 51 53 54 55 64 69 75 block 14 18 26 strip 14 17 18 26 41 51 53 54 55 64 69 75 environmental requirements 22 error logs 43 error message 61 67 export 18 37 43 44 46 device report 37 43 protocol 37 46 run log 37 44 F factory protocols 34 37 38 fitting the subassemblies of the instrument ...

Page 80: ...cation kit 19 70 R reagents 11 15 59 75 refitting the transport lock 24 30 31 releasing the transport lock 23 24 RNA 19 37 70 S samples 11 14 15 17 18 23 26 53 56 58 64 69 75 selecting the protocol 37 38 40 service 30 56 58 59 60 61 69 75 packing for 60 shield plate 18 29 55 58 75 shutting down 56 spare parts 17 70 specifications 3 11 63 general 60 63 performance 64 safety 61 64 65 switching on 33...

Page 81: ...Thermo Fisher Scientific Thermo Scientific KingFisher Duo User Manual 79 Notes ...

Page 82: ...80 Thermo Scientific KingFisher Duo User Manual Thermo Fisher Scientific Notes ...

Page 83: ......

Page 84: ...Thermo Fisher Scientific Oy Ratastie 2 P O Box 100 FI 01621 Vantaa Finland www thermoscientific com N12420 ...

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