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4.

Repeat these steps until you have specified the entire base sequence.

The displayed number of bases, %GC content, DNA Molecular
Weight, DNA absorptivity (extinction coefficient), and factor will be
updated as each new base is added to the sequence.

5.

Press the T

m

Calculation function key to view the melting point

calculator.

66 Evolution 300 and Evolution 600 User Guide

Thermo Fisher Scientific

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Summary of Contents for Evolution 300

Page 1: ...Evolution 300 and Evolution 600 User Guide ...

Page 2: ... the implied warranties of merchantability and fitness for a particular purpose Customers are ultimately responsible for validation of their systems 2005 2007 Thermo Fisher Scientific Inc All rights reserved No part of this publication may be stored in a retrieval system transmitted or reproduced in any way including but not limited to photocopy photograph magnetic or other record without our prio...

Page 3: ...ll holder 13 Test tube vial holder 15 Rectangular cell holder 15 Fiber optic sampling accessory 16 DRA EV 600 Diffuse Reflectance accessory 18 Solid Sample Holder 19 Smart Accessories 20 System accessories 21 Installing Smart Accessories 22 Using the Instrument 25 Local Control 25 Main Menu page 27 Text entry 37 Editing numeric fields 38 Pop up menus 39 File operation 39 Scan application 41 Fixed ...

Page 4: ...ing UVcalc 68 Instrument verification tests 71 VISIONsecurity and VISIONpro 74 Maintenance 75 Routine maintenance 75 Cleaning instrument exterior 75 Internal printer 76 Light sources Evolution 600 only 78 Deuterium lamp 80 Tungsten halogen lamp 81 Mercury lamp Evolution 300 and Evolution 600 82 ...

Page 5: ... an Evolution 300 or Evolution 600 spectrophotometer Our spectrophotometers integrate advanced hardware features with the power and flexibility of a wide range of Smart Accessories Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 1 ...

Page 6: ...tice to avoid damaging the system hardware or losing data Caution Indicates a hazardous situation which if not avoided could result in minor or moderate injury Warning Indicates a hazardous situation which if not avoided could result in death or serious injury Danger Indicates a hazardous situation which if not avoided will result in death or serious injury 2 Evolution 300 and Evolution 600 User G...

Page 7: ...k graphical symbol inside a yellow triangle with a black triangular band This is the general warning sign Failure to heed the safety precautions could result in personal injury White graphical symbol inside a blue circle This is the general data loss or property damage symbol and is not related to personal injury Failure to heed these precautions can result in irreparable damage to property or per...

Page 8: ... major components visible on the outside of a typical Evolution spectrophotometer Some components may not be present on your spectrophotometer Contrast Keypad LCD screen Ze ro Ru n 7 8 9 4 5 6 1 2 3 0 Optional printer Floppy disk drive Sliding door Sample compartment Port for optional power supply 4 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 9: ...o connect a printer with a parallel interface Key Bd Use to connect an English keyboard this is supplied by us to meet electrical emission requirements Ext Use to connect an external TTL or contact closure trigger Accy Use to connect an external accessory RS232 Use to connect a computer with an RS232 interface Ext Display Use to connect to a computer monitor Thermo Fisher Scientific Evolution 300 ...

Page 10: ...rophotometer and disconnect the power supply from the wall outlet or power strip before disconnecting the power supply from the spectrophotometer Inside sample compartment The following illustrations show the locations of the connectors inside the sample compartment Evolution 300 Reference connector Sample connector 6 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 11: ...Evolution 600 Sample connector Accessory detector connector Accessory detector connector Reference connector Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 7 ...

Page 12: ...ed and removed as follows Manual accessories are mounted on a common baseplate This system ensures correct alignment of the accessory within the instrument automatically These accessories may be installed or removed without the need to switch off the instrument and may be installed in the sample front position and or the reference rear position The accessory is positioned by two pins A and B at ei...

Page 13: ...d over pins A and B in the instrument When properly aligned gently push the front end of the accessory downward until plate snaps into place under the toggle This positive location system ensures that the accessory is correctly aligned in the corresponding optical beam For removal of the accessory Grip the accessory through the finger hole in the accessory and pull it upwards For accessories with ...

Page 14: ...small aperture cells The adjustment permits precise positioning of the cell in the beam 1 Install the accessory into the instrument as described above 2 Access the white light function in the operating software Local Control From the Main Menu page select Utilities then press the White Light function key on the Utilities page VISION Select Command White Light 10 Evolution 300 and Evolution 600 Use...

Page 15: ...light beam falls on the cell aperture Note Turning adjustment screw B clockwise moves the cell holder towards the front Turning it counterclockwise moves the cell holder towards the rear When the cell holder is aligned properly the white light will not be seen on the edges of the cell holder 5 Tighten securing screw A to secure the cell holder position When you have finished aligning the accessory...

Page 16: ...o 100 mm The capacity of the cell holder is adjusted by turning the knob on the front which alters the space between the gripping blocks Turn the knob until the space is longer than the pathlength required insert the cell and reduce the space until the cell is held securely Notice Do not overtighten 12 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 17: ...s designed to accommodate square cells with 8 8 mm z dimension and 10 mm pathlength and to operate at temperatures of 4 to 80 C 39 to 176 F Temperature control is achieved by means of an external temperature controlled circulator bath Refer to the Parts List supplied on CD with your instrument for details of available circulators A tubing kit for Evolution thermostatted accessories is available fo...

Page 18: ... and out tubes either so that they will both pass through one port or so that they pass on either side Replace the sample compartment front panel ensuring that the tubes are correctly located in the slit s and are not being pinched between the front panel and the body of the instrument Ensure that the tubes do not obstruct the light beam If necessary secure the tubes using the self adhesive tubing...

Page 19: ...ter and 40 to 100 mm high Note Exceeding the recommended diameter may cause the retaining bar to clip the light beam Rectangular cell holder This cell holder allows the use of 15 mm beam height filters and flow cells in the instrument Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 15 ...

Page 20: ...Fiber optic sampling accessory This accessory enables samples to be measured outside of the sample compartment 16 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 21: ...obe and allows the probe to be used with strong acids If contamination is an issue in the laboratory replacement tips are available The tip of the probe is manufactured to the same tolerances of a standard cuvette and are available in 5 10 and 20 mm pathlengths Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 17 ...

Page 22: ...utside the plane of the instrument A double beam Spectralon integrating sphere ensures high energy throughput and accuracy A transmittance port is also included for making diffuse transmittance measurements This DRA is fully CIE compliant for making color 18 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 23: ...es in Transmittance mode A variety of sample holders are available allowing round square and odd shaped samples to be measured The industry standard 2 x 3 sample slides allow other accessories to be used with the Solid Sample Holder Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 19 ...

Page 24: ...when purchased Smart Linear 16 Cell Changer This is a fast precise accessory for rapid measurement of up to 16 samples in manual semiautomatic or fully automatic modes It supports 4 channel cells and 4 channel flow cells Smart Thermostatted Rotary 7 Cell Changer Consists of a carousel also referred to as a cell holder installed on a base unit The base unit incorporates the drive motor control circ...

Page 25: ...nd visible stray light resolution noise and stability Absorbance and wavelength filters are traceable to NPL or NIST via an ISO standard 17025 accredited calibration process The CVC is supplied with a calibration certificate and shipping box for recalibration Mercury Lamp Provides a means of wavelength calibration and verification in the UV and visible spectral ranges using six fundamental mercury...

Page 26: ...nector and socket during installation 1 Open the main lid of the sample compartment and remove any accessory that may be present in the sample front position 2 Remove the front wall of the sample compartment by sliding it upward in its grooves 3 Insert the tapered guide pin on the bottom front of the accessory into the corresponding guide pin hole located next to the D connector female Do not pres...

Page 27: ...Thermostatted Single Cell Holder the alignment holes and pins are not visible due to the plastic cover over the unit 5 Press down on the front of the unit to complete the connection between the accessory and the D connector 6 Replace the front wall of the sample compartment Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 23 ...

Page 28: ...probe For removal of the accessory Grasp the handles of the accessory and pull it upwards The software will display a message confirming that the accessory has been removed Press Clear to remove this message 24 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 29: ...o analyze samples The topics that are discussed include Local Control software VISIONsecurity and VISIONpro Local Control Function keys are operational in the context for each screen and are shown at the bottom of the display Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 25 ...

Page 30: ...l prompts Zero Zero Perform the measurements specified in the current method Run Run Display the contents of the instrument library Library Clear error message or cancel pop up menus Clear Clear Confirm highlighted choices in menus and text entry boxes Enter Enter Select menu items and characters in text entry screens Arrow keys Enter numbers decimal point and minus sign Numeric keypad 26 Evolutio...

Page 31: ...er for internal printer if installed Feed Main Menu page The major functions of the software are accessed from the Main Menu page Use the up and down arrow keys to highlight the required menu item and press Enter to confirm your selection Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 27 ...

Page 32: ...en the six major applications and to view the contents of the Library or the contents of the disk currently in the drive Here is an example of a live status display 28 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 33: ...matically incremented for each sample Default File Type Allows the user to select the file type of the saved data Normal is the native file type of the software and can be imported into the VISION software package This file type can be used only by the Local Control or the VISIONpro VISIONsecurity software The comma separated values CSV format enables data to be exported to computer third party ap...

Page 34: ... printer Language Selects the appropriate display language Sound Toggled On any error is indicated by an on screen message and audible beep Toggled Off on screen messages are the only indication of an error History File Toggles On significant events e g recalibration are automatically recorded in the History File Toggled Off no events can be recorded in the History File Live Status Toggled On the ...

Page 35: ...to access the instrument Toggled Off no password is required to access the instrument Screen Colors Allows the user to select the colors used in the display Utilities window To access the Utilities window press the Utilities function key Highlight and press Enter to select a menu item Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 31 ...

Page 36: ...e initialization Ensure that a lifetime initialization is really needed before selecting this option Wavelength Calibration Performs a wavelength calibration using the optional mercury lamp accessory Ensure that a wavelength calibration is really needed before selecting this option Lamp Status Shows current status of the installed lamps Upgrade Software Upgrades instrument software from disk Initi...

Page 37: ... the accessories currently installed Here is an example Highlight the required accessory and press Enter to open the setup page When accessories are installed additional items related to the control of the accessories are added to the method parameter pages Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 33 ...

Page 38: ...ctrophotometer SmartStart The instrument can be configured so that frequently used methods are displayed on the SmartStart menu This then replaces the Main Menu as the Home page enabling common methods to be accessed conveniently from the start up screen Select files to be included in the SmartStart menu 1 Press Library 2 Highlight a file to be included in the SmartStart menu 3 Press the SmartStar...

Page 39: ...unction key has been pressed Continue in this way until all required files have been chosen for the SmartStart menu 4 Press Home All of the selected items appear on the SmartStart Menu page Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 35 ...

Page 40: ...To remove a file from the SmartStart menu Highlight the checked file s in the Library Press SmartStart 36 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 41: ... wrap so that for example pressing the left arrow from A will move the highlight to M 1 Press Enter to move the selected character into the edit field Continue until all the required characters are in place 2 Press the Accept function key Note Pressing the Cancel function key will close the text entry screen without making any changes Note The backspace function key may be used to erase mistakes T...

Page 42: ...Edit Value box Press Enter when the numeric entry is complete or Cancel to terminate numeric entry to close the Edit Value box without making any changes When setting up a page requiring multiple numeric entries e g a Standards page press the Accept function key when the page is complete or C to close the page without making any changes 38 Evolution 300 and Evolution 600 User Guide Thermo Fisher S...

Page 43: ...t Library or on a floppy disk The contents of the Library can be viewed accessed and maintained at any time by pressing the Library button on the instrument keypad Access the Library or disk from the Main Menu 1 Highlight the required field using the up down arrow keys and press Enter 2 Choose the required operation from the menu Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide ...

Page 44: ...Load Function key is selected the Library page is disabled showing the files that are appropriate to the current context 4 Press the Disk Library function key to display the contents of the disk if required 5 Highlight the required file by using the up down arrow keys and then press Load File function key 40 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 45: ...name optional Drive Allows the user to select whether the method is saved to disk or instrument Library Press the Save function key available after a filename has been entered to save or Clear to exit without saving Scan application Follow these steps to run a Scan application 1 Start the Scan application To do this highlight Scan on the Main Menu page and press Enter The Scan method page will ope...

Page 46: ...up a Peak Table for peak operations that will be performed at run time Graph High Enter maximum values for the graph y axis Graph Low Enter minimum values for the graph y axis Smoothing Selects the degree of smoothing required at run time UVcalc Refer to Using UVcalc Alternatively these operations are all available as post run manipulations 3 Set up any Accessory Methods required only when a Smart...

Page 47: ...nted samples from sample compartment 7 Insert a suitable reference if required and close the lid of the sample compartment 8 Press Zero to measure a baseline 9 Open lid of the sample compartment and insert the sample and then close the lid 10 Press Run The Scan Graph page will appear 11 Press the Manipulate function key to open the Manipulate menu and choose the post run manipulation required Ther...

Page 48: ...For example press the View Results function key to see the results of Peak Table operations or post run manipulations 44 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 49: ...w these steps to run a Fixed application 1 Highlight Fixed on the Main Menu page and press Enter The Fixed Method page will open The menu may contain more items than are shown in the example depending on the way the software is configured and which accessories are installed Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 45 ...

Page 50: ...nt time Timers Enables up to four countdown timers to be incorporated into the method sequence A timer title allows the user to specify the action e g shake boil associated with the timer At the end of the timer period the program may continue to the next step or pause to await action Timers may be multiple use or single use UVcalc Refer to Using UVcalc Use the table below to help you choose betwe...

Page 51: ...r the beams or insert suitable references and close the lid of sample compartment 5 Press Zero to zero the instrument 6 Open sample compartment lid and insert the samples 7 Close the lid of the sample compartment and press Run 8 Press the View Results function key to see the Fixed Results Table From this page it is possible to save the results to the instrument Library or to disk Thermo Fisher Sci...

Page 52: ... depending on the way the software is configured and which accessories are installed 2 Set up the method and press Enter Highlight each parameter in turn This will cause the item to toggle between two values or open a menu or open a numeric entry box Seven different data modes are available The number of wavelengths required will depend on the Data Mode 48 Evolution 300 and Evolution 600 User Guid...

Page 53: ... 3 Quadratic through 0 2 Normally a calibration contains more than the minimum number of standards Timers and UVcalc equations can be used if required 3 Press the Load Method function key to load a previously saved method from the instrument Library or from disk 4 Clear the beams or insert suitable blank s and close the lid of the sample compartment 5 Press Zero to zero the instrument 6 Press the ...

Page 54: ...itation method 10 Press the View Results function key to see the Results Table Function keys on the Quant Results page enable you to save the results to the instrument Library or to disk or to clear the results table ready for a new batch of samples Kinetics application Follow these steps to run a Kinetics application 1 Start the Kinetics application and press Enter The Kinetics Method page will o...

Page 55: ...or the specified Total Measurement Time and the difference calculated The Cycle Time is the interval between measurements at the same wavelength Parallel Kinetics Only available with cell changer The absorbance of each sample is measured in turn for the specified number of cycles The Cycle Time is the interval between measurements on the same cell Total Measurement Time is calculated from the numb...

Page 56: ...he first measurement enter a delay time Choose whether to display the absolute values of the absorbance or the change in the absorbance relative to the initial measurement If Relative absorbance is chosen an additional menu item appears to set positive or negative slope Set up Graph High and Graph Low to accommodate the expected absorbance range The graph can be reconfigured post run if required E...

Page 57: ... the Pause function key is selected again data collection will resume There will be a gap in the Kinetics Graph for the duration of each pause In Parallel Rate the pause will not take effect until the current cycle of measurements has been completed 8 Press the Manipulate function key to access the Manipulate menu 9 Rescale smooth or change the absorbance display if required Select Original to ret...

Page 58: ...n key to save the results to the instrument Library or to disk Multicomponent application Follow these steps to run a Multicomponent MCA application 1 Start Multicomponent Analysis and press Enter The MCA method page will open The menu may contain more items than are shown in the example depending on the way the software is configured and which accessories are installed 54 Evolution 300 and Evolut...

Page 59: ...ame method Each file must contain data for a single sample Use Clear Results between Fixed measurements Standards Transfers to the MCA standards page When Measure Standards Yes enter an ID and concentration for each standard When Measure Standards No load Standards as described above and enter a concentration for each standard Wavelengths When Measure Standards Yes enter the wavelengths at which s...

Page 60: ...ured at each of the wavelengths specified in the method The concentration of each component is then calculated from the calibration 7 Press the View Results function key to transfer to the MCA Results page The up down arrow keys can be used to view previous following results 8 Press the Save Results function key to save results to the instrument Library or to disk Biological tests Follow these ste...

Page 61: ...ess Enter Choose the required test from the Nucleic Acid Tests sub menu The relevant test page opens The content and layout of the page will depend on the test selected the way the software is configured and which accessories are installed Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 57 ...

Page 62: ...n and press Enter to toggle Off On When On a Reference Wavelength is added to the parameter list 5 Highlight Display Protein and press Enter to toggle Yes No When Yes the protein factors are displayed 6 Edit the Dilution Multiplier if required 7 Edit the Units if required 8 When using a DNA method with scan press the Setup Scan function key 58 Evolution 300 and Evolution 600 User Guide Thermo Fish...

Page 63: ...tment 10 Press Zero A message Measuring Baseline appears 11 Insert the sample in the cell holder and close the lid of the sample compartment 12 Press Run A message Measuring Sample appears Note Quartz cells MUST be used for all DNA measurements Glass or plastic cells are not suitable because they do not transmit at the wavelengths used The results page contains the results of the calculations and ...

Page 64: ...t Protein Tests and press Enter Choose the required test from the Protein Tests sub menu The relevant test page opens The content and layout of the page will depend on the test selected the way the software is configured and which accessories are fitted The method parameters are documented values published in the literature Parameters may be edited if required 60 Evolution 300 and Evolution 600 Us...

Page 65: ...blank s and close the lid of the sample compartment 7 Press Zero A message Zeroing appears 8 Press the Calibrate function key and follow the on screen instructions to perform the calibration 9 Save the calibrated method to the instrument Library or to disk using a new file name Note A previously calibrated method can be loaded from the instrument Library or from disk Press the Load Method function...

Page 66: ... Results function key on the Results page Measure cell growth The cell growth measurement uses absorbance at 600nm to indicate the progress of cell growth in a sample The instrument does not perform any calculations or graph the data 1 Highlight Biological Tests on the Main Menu page The Biological Tests sub menu will open 2 Choose Cell Growth and press Enter 62 Evolution 300 and Evolution 600 Use...

Page 67: ... beams or insert suitable blank s and close the lid of the sample compartment 4 Press Zero A message Zeroing appears 5 Place the unknown s in the correct cell position s and close the lid of the sample compartment 6 Press Run The sample number and absorbance are displayed on the Results page Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 63 ...

Page 68: ...weight Absorptivity extinction coefficient Conversion factor to be used in oligonucleotide measurements Tm for oligos up to 20 mers DNA DNA hybrids and RNA hybrids 1 Highlight Biological Tests on the Main Menu page The Biological Tests sub menu will open 2 Choose Oligo Calculator and press Enter 64 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 69: ...ou will need to enter a base sequence before you can run the oligonucleotide calculations With the base sequence screen displayed press the right left arrow keys to select the required base Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 65 ...

Page 70: ... bases GC content DNA Molecular Weight DNA absorptivity extinction coefficient and factor will be updated as each new base is added to the sequence 5 Press the Tm Calculation function key to view the melting point calculator 66 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 71: ... press Enter A pop up menu will appear Here is an example Once all parameters have been set appropriately the relevant set of melting point predictions will be displayed Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 67 ...

Page 72: ... calculations to be incorporated into Scan Fixed and Quant methods 1 Highlight UVcalc on the selected application page and press Enter The equation selection page opens 68 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 73: ...tarting a UVcalc calculation and press Enter The equation entry page for the selected equation opens with the Formula line highlighted 3 Press Enter to edit the formula The formula page opens Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 69 ...

Page 74: ...ets are supported Operators Add subtract multiply divide spaces may be used if required 4 Use the arrow keys to move the highlight to the required element and press Enter Further options will be made available as required The back arrow and function keys may be used to edit the equation 5 When the equation is set up press the Accept function key The Cancel button enables you to exit without changi...

Page 75: ...ght the item and pres Enter and then enter the test and or numbers in the usual way Instrument verification tests The menu may contain fewer items than are shown depending on the accessories installed Note Turn on the instrument at least an hour before starting performance qualification tests 1 From the Main Menu page press the Utilities function key 2 Highlight Instrument Verification and press E...

Page 76: ...nd press the Select Deselect function key to select it Here is an example 4 Press Run after selection s has been made Note The drift test will take at least an hour 72 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 77: ... to disk Previous results can be loaded using the Load Results function key or by using the Library or disk menu items on the Main Menu page Further tests will become available when an instrument verification accessory CVC or Mercury lamp is installed Refer to the appropriate accessory manual Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 73 ...

Page 78: ...mprehensive on line Help system on the VISION CD or To start VISION you can either Double click on the VISION icon on the Windows desktop Select Start Programs VISIONpro VISIONpro Start Programs VISIONsecurity VISIONsecurity By default VISION uses the Com 1 port on the computer 74 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 79: ...cleaned regularly Wipe off any spilled chemicals immediately Cleaning instrument exterior The exterior and sample compartment of the instrument can be cleaned periodically as follows Caution Do not allow moisture to leak into the instrument 1 Switch off the spectrophotometer and disconnect from AC power source 2 Using a lint free cloth dampened with a weak solution of detergent and water wipe the ...

Page 80: ... housing To do this lift the cover and swing it away from the display Lift cover up swing back 2 Turn the paper release lever through 90 towards the display 3 Feed the paper shiny side down into the input slot Ensure that the paper emerges correctly through the printer and approximately 10 cm 4 in has been pulled clear 76 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 81: ...Lift paper release 4 Push the paper release lever back into place 5 Replace the printer housing Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 77 ...

Page 82: ...en halogen and deuterium lamps get very hot while the instrument is operating Before removing either lamp turn off the instrument and allow it to cool for 15 minutes CAUTION 78 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific ...

Page 83: ...outlet or power strip Warning The following lamp replacement procedures require removal of the lamp area cover Operation of the instrument with the optics cover removed exposes the operator and other observers to ultraviolet radiation which can damage the human eye and cause burning of the skin All personnel in the area must wear protective UV absorbing safety glasses Thermo Fisher Scientific Evol...

Page 84: ... screws 2 turns counterclockwise b Rotate the lamp and pull upward until out of housing c Unplug inline connector 4 Insert new lamp Caution Never touch the bulb with your fingers Oil from your skin may cause the bulb to burn out quickly or explode a Plug in inline connector b Place lamp in lamp housing aligning hex screws c Using 2 5 mm hex driver tighten screws 5 Replace rear cover 6 Plug in the ...

Page 85: ...en the two securing screws b Holding the lamp socket by the mounting tabs remove the copper spring clip c Unplug the tungsten halogen lamp from socket 4 Insert new bulb Caution Never touch the bulb with your fingers Oil from your skin will cause the bulb to burn out quickly or explode a Holding the lamp socket by the mounting tabs insert the bulb pins into the socket b Replace the copper spring cl...

Page 86: ...spectrophotometer to the AC outlet and turn on the power switch Note Lamp hours and energy if applicable must be reset from the controlling software Mercury lamp Evolution 300 and Evolution 600 Refer to the Mercury Lamp Accessory user s guide for directions on replacing the mercury lamp ...

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