3.
Load a previously saved method from the instrument Library or
disk.
Press the
Load Method
function key, select the required file and load it.
4.
Clear the beams or insert appropriate blank(s) and close the lid of
the sample compartment.
5.
Press
Zero
.
6.
Load the cell holder, initiate the reaction(s) as necessary and close
the lid of the sample compartment.
7.
Press
Run
.
The method will be executed and the absorbance(s) of the sample(s)
will be plotted on the Rate Graph.
When the Pause function key is selected, data collection will cease but
the clock will continue to run. When the Pause function key is selected
again, data collection will resume. There will be a gap in the Kinetics
Graph for the duration of each pause. In Parallel Rate, the pause will
not take effect until the current cycle of measurements has been
completed.
8.
Press the
Manipulate
function key to access the Manipulate menu.
9.
Rescale, smooth or change the absorbance display, if required.
Select
Original
to return to the unmanipulated data.
10.
Set up the rate calculations.
Section allows up to 5 markers to be placed on the graph defining up to
4 contiguous time intervals over which the rate statistics will be
calculated.
When the markers have been placed, press the function key to exit
from the Section Mode.
Thermo Fisher Scientific
Evolution 300 and Evolution 600 User Guide 53
