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Thermo

Agilent 1200 Series

Getting Connected Guide for LC Devices

XCALI-97250 Revision H

April 2014

Summary of Contents for Agilent 1200 Series

Page 1: ...Thermo Agilent 1200 Series Getting Connected Guide for LC Devices XCALI 97250 Revision H April 2014 ...

Page 2: ...cted and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information recei...

Page 3: ... Using Default 7 Setting the Stored TCP IP Address for the LAN Interface 9 Setting the LAN Initialization Mode to Using Stored 16 Chapter 3 Setting Up the Thermo Foundation Instrument Configuration 19 Agilent 1200 Series LC System IP Address 19 Contact Interface Board 20 Configuring the Agilent LC System s Modules 20 Adding the Agilent 1200 Series Modules to the Foundation Instrument Configuration...

Page 4: ...or LC Devices Thermo Scientific Chapter 6 Triggering Data Acquisition 37 Chapter 7 Vial and Well Locations for the Available Tray Types 39 40 Vial Trays 40 100 Vial Tray 41 54 Vial Plates 42 96 Well Plates or 96 Deep Well Plates 43 384 Well Plates 44 Index 45 ...

Page 5: ...the data system computer and how to establish communication between the LC system and a Thermo Scientific MS data system such as Thermo Xcalibur To suggest changes to the documentation or to the Help Complete a brief survey about this document by clicking the button below Thank you in advance for your help Contents Related Documentation System Requirements Supported Firmware Versions Cautions and ...

Page 6: ... Programs Thermo Instruments Manuals LC Devices Agilent For a Thermo Scientific MS choose Start All Programs Thermo Instruments Instrument name For the Xcalibur application choose Start All Programs Thermo Xcalibur Manuals Xcalibur For access to the Xcalibur application Help follow this procedure To view application specific Help From the Xcalibur Instrument Setup window choose Help Agilent 1200 H...

Page 7: ...that you want to install System Minimum requirements Computer 2 GHz processor with 1 GB RAM DVD drive 80 GB or greater available on drive C Video card and monitor capable of 1280 1024 resolution Network interface cards two NTFS format Software Adobe Reader 10 Microsoft Windows operating system Windows 7 Professional 32 bit and 64 bita Windows XP Workstation SP3 Thermo Scientific software a LC Devi...

Page 8: ...ith Foundation go to step 3 If it is not compatible do the following a Use the Windows Control Panel to uninstall all of the modules from LC Devices b Install the compatible version of LC Devices c Restart the data system computer 3 If the installed LC Devices software is compatible with Foundation do the following a Open the Windows Control Panel b In the top Search box type Change User Account C...

Page 9: ...tem Module Model number Firmware version High Performance Autosampler and Thermostat G1367B G1330B A 06 12 High Performance Autosampler SL and Thermostat G1367C G1330B A 06 12 Micro Well plate Autosampler G1377A A 06 03 Thermostatted Column Compartment G1316A A 06 10 Thermostatted Column Compartment SL G1316B A 06 10 Binary Pump G1312A A 06 10 Binary Pump SL G1312B A 06 10 Capillary Pump G1376A A ...

Page 10: ...s in the Agilent 1200 Series Getting Connected Guide for LC Devices CAUTION Highlights hazards to humans property or the environment Each CAUTION notice is accompanied by an appropriate CAUTION symbol IMPORTANT Highlights information necessary to prevent damage to software loss of data or invalid test results or might contain information that is critical for optimal performance of the system Note ...

Page 11: ...mail us customer support analyze thermofisher com Web for product information www thermoscientific com lc ms Web for customizing your service request 1 From any Products Services web page click Contact Us 2 In the Contact Us box complete the information requested scroll to the bottom and click Send User Documentation Web for downloading documents mssupport thermo com 1 On the Terms and Conditions ...

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Page 13: ...ce drivers provided with LC Devices To check the firmware version of the LC modules 1 Make sure that the modules are connected by controller area network CAN communication cables that all the modules are turned on and that the Agilent Instant Pilot is connected For instructions on how to connect the CAN cables refer to the Agilent 1200 Series LC reference manuals 2 From the Welcome screen of the I...

Page 14: ... Info View To check the firmware versions of the LC modules from the Xcalibur data system 1 Establish communication with the Agilent LC system as follows a Connect the Agilent local area network LAN interface to the data system computer See Connecting the Ethernet Cables on page 34 b Configure the Agilent LAN interface to use a stored IP address See Chapter 2 Setting Up the LAN Interface c Specify...

Page 15: ...open the Information view and then click the Status tab 4 On the Status page click the module name to view its status Figure 3 Figure 3 Status page of the Xcalibur Roadmap View example Note To trigger data acquisition from a Thermo Scientific mass spectrometer you must install a contact closure board in one of the Agilent modules and connect the board to the Start In pins on the mass spectrometer ...

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Page 17: ...ides two options for the LAN interfaces On board LAN interface which might be the standard configuration or you can order as part of a module Agilent G1369 LAN card which you can order separately Note For more information refer to the user manual for the Agilent LAN card Contents Determining the LAN Interface Type and Location Setting the LAN Initialization Mode to Using Default Setting the Stored...

Page 18: ...ample Note If the LC stack does not have a built in LAN interface Agilent recommends that you install the Agilent G1369 LAN card in the detector if part of the system If an Agilent detector is not part of the system install the LAN card in the pump or autosampler module For instructions refer to the manuals that came with the Agilent 1200 Series LC system LAN USB REMOTE RS 232 CAN CAN CONFIG REMOT...

Page 19: ...dress To set the on board LAN interface s initialization mode to use the default IP address Set the initialization switches on the back panel Figure 6 as follows Figure 6 Back panel on board LAN configuration switches set to the default IP address To set the LAN card s initialization mode to use the default IP address 1 For the module that contains the LAN card turn off the power switch and unplug...

Page 20: ...witch is up and the Off position is down With these switch settings the LAN card uses the factory default settings to enable a Transmission Control Internet Protocol TCP IP connection to the LAN interface with a Telnet session Figure 7 Configuration switches on the Agilent G1369 LAN card 6 Reinstall the LAN card 7 Using the Category 5 Ethernet cable connect the LAN card directly to the dedicated E...

Page 21: ...t the LAN interface connects to the data system computer network card labeled Surveyor MS 3 Open the Internet Protocol Properties dialog box as follows a Depending on the operating system open the Local Area Connection Status dialog box as follows Figure 8 Microsoft Windows 7 or later Choose Start Control Panel Network and Internet or Networking and Sharing Center if set to the Icon view and then ...

Page 22: ...s for the LAN Interface 10 Agilent 1200 Series Getting Connected Guide for LC Devices Thermo Scientific Figure 8 Local Area Connection Status dialog box Windows 7 and XP b Click Properties to open the Local Area Connection Properties dialog box Windows XP Windows 7 ...

Page 23: ...tting Connected Guide for LC Devices 11 c Do one of the following Figure 9 Windows 7 or later On the Networking page select the Internet Protocol Version 4 TCP IPv4 check box or Windows XP On the General page select the Internet Protocol TCP IP check box Figure 9 Local Area Connection Properties dialog box Windows XP Windows 7 ...

Page 24: ...re 10 Internet Protocol Version 4 TCP IPv4 Properties dialog box with the IP address for the dedicated Ethernet card 4 Set the IP address as follows a Select the Use the Following IP Address option b In the IP Address box type 192 168 254 100 c In the Subnet Mask box type 255 255 0 0 d Click OK 5 Windows 7 or later Enable Telnet as follows a Choose Start Control Panel Programs and Features Icon vi...

Page 25: ...254 11 and then press ENTER Figure 11 Figure 11 Command Prompt window with the default IP address The Telnet session with the Agilent LAN interface begins Figure 12 Figure 12 Beginning of a Telnet session with the Agilent LAN interface 7 Set the IP address for the Agilent LAN interface as follows a At the command prompt Figure 12 type a slash and then press ENTER to display the LAN interface s cur...

Page 26: ...d subnet mask settings type a slash and then press ENTER to display the current settings of the LAN interface 8 To exit the Telnet session do one of the following For the on board LAN interface type exit at the prompt or For the LAN card type quit at the prompt quit Saves changes and exits the shell for the G1369 LAN card exit Model G1369 LAN card Exits the Windows command line without saving the ...

Page 27: ... a Open the Internet Protocol Version 4 TCP IPv4 Properties dialog box see step 3 on page 9 Figure 14 Internet Protocol Version 4 TCP IPv4 Properties dialog box b In the IP Address box type 172 16 0 101 c In the Subnet Mask box type 255 255 0 0 d Click OK e Exit the Control Panel After setting the stored IP address and subnet mask for the Agilent LAN interface you are ready to change the LAN inter...

Page 28: ... LAN card s initialization mode to use the stored IP address To set the on board LAN interface s initialization mode to use the stored IP address Set the initialization switches on the back panel Figure 6 on page 7 as follows The On position for the toggle switch is up and the Off position is down To set the LAN card s initialization mode to use the stored IP address 1 For the module that contains...

Page 29: ...switches on the LAN card Figure 7 on page 8 as follows The On position for the toggle switch is up and the Off position is down 6 Reinstall the LAN card 7 Using the Category 5 Ethernet cable connect the LAN card directly to the dedicated Ethernet port in the data system computer 8 Plug in the disconnected power supply cord and then turn on the LC modules Switch 4 5 6 Position Off On Off ...

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Page 31: ...2 for the Agilent LC system when you configure the modules After you enter the stack IP address for one module this IP address appears in the configuration dialog box for each connected device Contents Agilent 1200 Series LC System IP Address Contact Interface Board Configuring the Agilent LC System s Modules IMPORTANT To maintain stable communication with the Thermo Scientific data system you mus...

Page 32: ... When you specify the configuration options for the LC system select the Contact Board Installed check box for the module that contains this board Configuring the Agilent LC System s Modules To specify the configuration options for the LC modules follow these procedures Adding the Agilent 1200 Series Modules to the Foundation Instrument Configuration Setting the Configuration Options for the LC Mo...

Page 33: ... each module that you want to add to the Configured Devices area 4 Configure the selected modules See the next section Setting the Configuration Options for the LC Modules To set the configuration options for the Agilent LC modules that the Foundation platform supports follow these procedures Configuring the Agilent 1200 Series Autosampler Configuring the Agilent 1200 Series Pump Configuring the A...

Page 34: ...er Configured Devices double click the autosampler icon and then do the following on the General page Figure 16 Figure 16 General page of the Agilent 1200 G1367 HiP ALS dialog box example If you installed the contact interface board in the autosampler select the Contact Board Installed check box In the Stack IP Address box make sure that the IP address is 172 16 0 102 All of the Agilent LC modules...

Page 35: ...ation select the tray type For information about the vial and tray locations see Chapter 7 Vial and Well Locations for the Available Tray Types If the Agilent stack has an autosampler thermostat module G1330B select the Thermostat Control Installed check box 4 Click OK Configuring the Agilent 1200 Series Pump Use the pump s configuration dialog box to specify its serial number and model number For...

Page 36: ...lent 1200 LC modules in the stack share the TCP IP settings Changing the value of a setting for one module in the Instrument Configuration window changes the value of that setting for all modules in the stack In the Serial Number box type the serial number that is located on the front lower right corner of the Agilent module Because you can configure two pumps per stack you must type the correct s...

Page 37: ...of the Agilent 1200 Capillary Pump dialog box 5 Click OK Configuring the Agilent 1200 Series Thermostatted Column Compartment Use the thermostatted column compartment TCC configuration dialog box to specify its model number To specify the configuration options for the TCC 1 Follow the procedure To add the LC modules to the Foundation instrument configuration on page 20 2 In the Instrument Configur...

Page 38: ...gured Devices double click the detector icon and then do the following on the General page Figure 21 If you installed the contact interface board in the detector select the Contact Board Installed check box In the Stack IP Address box make sure that the IP address is 172 16 0 102 Note All Agilent LC modules in the stack share the TCP IP settings Changing the value of a setting for one module in th...

Page 39: ...the front lower right corner of the module Figure 21 General page of the Agilent 1200 G1315 DAD dialog box example 3 Click OK Closing Foundation Instrument Configuration Before opening the Thermo Scientific data system close the Foundation Instrument Configuration window You cannot have both applications open at the same time To close the Foundation Instrument Configuration window After you config...

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Page 41: ...rometer the Agilent 1200 Series autosampler or pump must have an external contact binary coded decimal BCD interface board Figure 22 For information about ordering the BCD board refer to the manuals supplied with the Agilent LC modules Figure 22 External contact interface board BCD board installed in an Agilent pump 1 8 CAN CAN HP 1B CONFIG RELAY CONTACTS REMOTE VIAL OUTPUT RS232 Screws that secur...

Page 42: ...ormation about preventing ESD damage caused by an electrical discharge 3 Using a slotted screwdriver remove the back cover plate over the empty board slot 4 Insert the contact interface board into the slot and then tighten the two screws to secure the board to the enclosure Figure 22 on page 29 This completes the installation of the contact interface board CAUTION To prevent personal injury caused...

Page 43: ...s a Connect the external contact trigger cable b When you configure the module with the contact interface board see Configuring the Agilent LC System s Modules on page 20 make sure that you select the Contact Board Installed check box c When you create an instrument method for the LC MS system make sure that you create an appropriate time program for the trigger signal See Chapter 6 Triggering Dat...

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Page 45: ... LAN card Model G1369 unless the Agilent LC system contains a module with an on board LAN communication interface Figure 24 Agilent BCD board for contact closure Contents Ethernet Communication Kit Connecting the Ethernet Cables Connecting the Contact Closure Cable Table 3 Ethernet Communication Kit P N OPTON 30012 Description Part number Agilent BCD contact interface board 00012 27714 Contact clo...

Page 46: ...le from the Ethernet switch to the Ethernet network card in the data system computer that is dedicated to the LC MS system typically network interface card number 3 CAUTION Safety and EMC regulations require the use of Category 5 shielded Ethernet communication cables maximum 3 m 10 ft long REMOTE RS 232 CONFIG ANALOG CAN CAN Agilent Technologies Activity Speed G1369 LAN Interface LAN USB REMOTE R...

Page 47: ...ct closure cable to the mass spectrometer 1 Follow the procedure To install the external contact interface board on page 30 2 Using an appropriate connector for the mass spectrometer connect the white wire from the contact closure cable to the Start In pin and the brown wire to the Start In pin on the mass spectrometer s input output I O panel Figure 26 3 Connect the cable s DB15 plug to the RELAY...

Page 48: ...n the Agilent contact interface board and the Thermo Scientific MS START IN START IN Start In Start In START IN 5V Max 5V Max right side panel left side panel White wire Brown wire TSQ Series MS back panel LCQ Fleet LTQ Series or LXQ MS right side panel Surveyor MSQ MS back panel LCQ Series MS back panel Not drawn to scale Contact closure cable connects to the relay contacts on the contact interfa...

Page 49: ...n 2 Click the icon for the LC module that contains the contact interface board typically the autosampler to open its Instrument Setup window 3 Click the Timed Events tab CAUTION To prevent a data system failure make sure that you disconnect the Agilent Instant Pilot cable from the LC stack before you run data acquisitions or use the direct control commands Note Instrument methods contain the conta...

Page 50: ...t Closed from the list Figure 27 b In row 2 do the following i Select the Time min cell and then type 0 10 ii Select the Contact A cell and then select Open from the list c Make sure that all the other Contact cells display Open Figure 27 Table on the Timed Events page 5 Make the appropriate entries and selections for the rest of the instrument method 6 Save the instrument method with an appropria...

Page 51: ...ack is the factory default setting for the orientation of plates plate rotation setting For the Left Back plate rotation the A1 well location is to the left and at the back of the plate You can change the plate rotation to Right Front by using the Agilent Instant Pilot controller For instructions refer to the reference manual for the Agilent LC stack Contents 40 Vial Trays 100 Vial Tray 54 Vial Pl...

Page 52: ...he left and right trays Figure 28 shows the vial locations for the 40 vial trays When you create a sequence you can type a vial location from 1 to 40 for a single tray or 1 to 80 for two trays If you turn on the wash option for an instrument method you can specify a wash vial from 1 to 40 or 1 to 80 depending on whether you load one or two trays into the tray compartment Figure 28 Two 40 vial tray...

Page 53: ...ray into the autosampler s tray compartment The Xcalibur data system recognizes this tray as the left tray Figure 29 shows the vial locations for the 100 vial tray When you create a sequence you can type a vial location from 1 to 100 If you turn on the wash option for an instrument method you can specify a wash vial from 1 to 100 Figure 29 100 vial tray 1 91 Row 1 Row 10 Column 1 Column 10 ...

Page 54: ...ting of Left Back for the plate rotation When you create a sequence you can type a vial location from 1 A1 to 1 F9 for the front plate from 2 A1 to 2 F9 for the back plate and from 3 A1 to 3 J1 for the vials If you turn on the wash option for an instrument method you can specify a well location from 1 A1 to 2 F9 or a reservoir location from 1 to 10 Figure 30 Two 54 vial plates and one vial column ...

Page 55: ...ting of Left Back for the plate rotation When you create a sequence you can type a well location from 1 A1 to 1 H12 for the front well plate from 2 A1 to 2 H12 for the back well plate and from 3 A1 to 3 J1 for the vials If you turn on the wash option for an instrument method you can specify a well location from 1 A1 to 2 H12 or a reservoir location from 1 to 10 Figure 31 Two 96 well plates and one...

Page 56: ...ial locations for the 2 mL vials When you create a sequence you can type a well location from 1 A1 to 1 P24 for the front well plate from 2 A1 to 2 P24 for the back well plate and from 3 A1 to 3 J1 for the vials If you turn on the wash option for an instrument method you can specify a well location from 1 A1 to 2 P24 or a reservoir location from 1 to 10 Figure 32 Two 384 well plates and one vial c...

Page 57: ...uirements vii configuring the modules See Agilent 1200 Series contact interface board autosampler 22 detector 26 device drivers 20 installing 30 pump 24 contacting us xi cover removing from a module 7 30 D data acquisition 37 data acquisitions triggering 37 detector configuring 26 device drivers vii direct control commands note for 37 documentation accessing vi additional vi E EMC compliance 34 er...

Page 58: ...le connecting 35 I O panel 35 mass spectrometry applications v module name 24 P pump configuring 24 R relay contacts connecting the contact closure cable 35 location of 29 release notes vii requirements computer vii software vii S server failure error message viii software requirements vii stack IP address 22 24 26 subnet mask for the Surveyor MS network card 12 15 system failure preventing 37 sys...

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