TRIO MPC-165 SERIES MICROMANIPULATOR SYSTEM OPERATION MANUAL – REV. 2.67K2 (20201130) (FW V2.62)
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The TRIO MPC-100’s ROE provides fine control of electrode position and the rate of rotation
of ROE dials for each axis determines the speed of travel. The finest step size is less than
100nm. Five conveniently located buttons on the ROE provide control of all the basic
functions you will need in normal operation (Work, Home, Speed/Lock, Relative, and
Pulse/Angle).
Press and hold WORK (for 3 seconds) to quickly store a work position, tap HOME to move all
axes to an initial location that is useful for changing electrodes, or press and hold the HOME
button (for 3 seconds) to memorize a new HOME position.
When ready to record data, suppress the motor drive electronics by pressing and holding for
3 seconds the SPEED/LOCK button. In the LOCK mode, the display turns red and ROE
input is locked out to avoid any accidental motion.
Pressing and holding the RELATIVE button for three seconds at any location causes the
display coordinates to change to all zeroes. When activating relative mode, the display turns
blue.
To return to viewing the absolute coordinates, tap the RELATIVE button to toggle back to
absolute positioning mode.
Finally, tapping the PULSE button causes a 3
μ
m advance in the diagonal. This rapid burst of
forward motion can assist in sharp electrode cell penetration. Pressing and holding the
PULSE button for 3 seconds allows for entering the angle to which the electrode/headstage is
physically set so that X & Z coaxial (diagonal) movement is made at the correct angle.
All the electronics, except for a small power supply, are housed within the TRIO MPC-100
ROE and no separate controller or computer is required.
External computer control of the TRIO MPC-100 is possible via the USB connector mounted
on the controller/ROE’s rear panel. The controller’s internal software is programmed with a
defined set of commands allowing for a wide range of micromanipulator/stage movements as
programmed in software residing in an external computer connected via USB.
Designed with maximum flexibility in mind, a DIP switch on the controller changes the
directional movement of the ROE knobs to accommodate the preference of the user. A Y-axis
lockout function (accessible by DIP switch) is also available, allowing X/Z-only axial
movement during HOME and WORK repositioning. The TRIO comes standard with a
universal mounting system suitable for the most popular headstages or pipette holders.
The new MP-865/M micromanipulator features construction based on the TRIO-845, but
with the addition of precision cross-roller bearings for even tighter tolerances, and a 25pin
connector for future compatibility with our other controllers. The MP-865/M is a highly
stable 3-axis manipulator with 50 mm of travel on X axis, 12.5 mm on Y, and 25 mm on Z.
The TRIO MPC-100 controller gives the MP-865/M a synthetic fourth axis that can be set in
software to any angle between 0 and 90 degrees for diagonal movement. Based on a lead-
screw design with a smaller overall size and footprint than most manipulators, the MP-865/M
is ideal for applications that require 2 pipettes in one setup or for setups where space is
limited.