Geode H14000 User Manual
5
Intended use of the naica® system
The naica
®
system for Crystal Digital PCR
TM
is composed of two instruments: the Geode, which
performs droplet generation and amplification, and the Prism3, which enables imaging of the
droplet crystals. The Crystal Reader software is used to control and
set up Crystal Digital PCR™
experiments on the Prism3 instrument.
The Crystal Miner software is used to extract data from the images acquired using the Prism3
and to calculate the absolute concentrations of the targeted nucleic acids. This software is pre-
installed on the naica® PC delivered with the naica
®
system.
The naica® system performs
Crystal Digital PCR™ within microfluidic chips (Sapphire chips &
Opal chips).
The naica® PCR MIX reagents are recommended for use to achieve optimal Crystal Digital
PCR™ performance on the naica® system.
For detailed instructions for Prism3 & Crystal Reader software and Crystal Miner software, please
refer to the respective User Manual. For detailed instructions for Sapphire chips, Opal chips,
naica® PCR MIX, and naica® multiplex PCR MIX refer to the respective Instruction for Use (IFU).
The technical documentation is available here
https://www.stillatechnologies.com/technical-
resources/naica-system-prism3/.
The naica
®
system is a laboratory instrument to be used by qualified personnel in a controlled
environment. Before using the naica
®
system, the user should be trained by a Stilla Technologies
representative.
The naica
®
system is intended for Research Use Only. Not for use in diagnostic procedures.
Stilla Technologies recommends installing the devices in a clean, low-lint environment at an
optimal operating temperature between 15-25°C.
In general, Crystal Digital PCR™ can be performed with all types of DNA sample types on the
naica® system. However, individual sample-type compatibility for digital PCR applications may
require a dedicated assay validation by the end-user. Please note that the extraction method
used, and sample purity might influence sample compatibility for digital PCR applications as well.
For application-specific references please visit
https://www.stillatechnologies.com/applications/
Citing the naica® system in scientific publications, presentations, seminars, etc.
To cite the use of the naica
®
system use:
Crystal Digital PCR
™ (Stilla Technologies, France)
naica
®
system (Stilla Technologies, France)
naica® system component names:
•
Geode
•
Prism3
•
Sapphire chips
•
Opal chips
•
Crystal Reader software
•
Crystal Miner software
•
naica® PCR MIX reagents
o
naica® PCR MIX (Stilla Technologies, France)
o
naica® multiplex PCR MIX(Stilla Technologies, France).
