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TABLE OF CONTENTS 

INTRODUCTION ......................................................................................................................................................... 7

 

Read this Manual .................................................................................................................................................................... 7

 

Safety Considerations and Requirements ...................................................................................................................... 7

 

Contacting Assistance .......................................................................................................................................................... 8

 

Manufacturing Warranty ...................................................................................................................................................... 8

 

Engineering Improvements.................................................................................................................................................. 8

 

CO

2

 Gas Supply ...................................................................................................................................................................... 9

 

Reference Sensor Devices ................................................................................................................................................ 10

 

RECEIVING YOUR UNIT ...........................................................................................................................................11

 

Inspect the Shipment ............................................................................................................................................................ 11

 

Orientation Photos ............................................................................................................................................................... 12

 

Recording Data Plate Information ................................................................................................................................... 14

 

INSTALLATION ......................................................................................................................................................... 15

 

Installation Procedure Checklist ...................................................................................................................................... 15

 

Required Ambient Conditions ........................................................................................................................................... 16

 

Required Clearances ........................................................................................................................................................... 16

 

Power Source Requirements ............................................................................................................................................. 17

 

Lifting and Handling ............................................................................................................................................................ 18

 

Leveling ................................................................................................................................................................................... 18

 

Install the Incubator ............................................................................................................................................................. 19

 

Deionized and Distilled Water .......................................................................................................................................... 19

 

Installation - Clean and Disinfect ..................................................................................................................................... 19

 

Install Chamber HEPA Filter and Duct .......................................................................................................................... 20

 

Shelving Installation............................................................................................................................................................. 21

 

Connect to the CO

2

 

Supply .............................................................................................................................................. 22

 

Access Port Stopper ........................................................................................................................................................... 23

 

GRAPHIC SYMBOLS ............................................................................................................................................... 25

 

CONTROL PANEL OVERVIEW .............................................................................................................................. 27

 

OPERATION.............................................................................................................................................................. 29

 

Theory of Operation ........................................................................................................................................................... 29

 

Put the Incubator into Operation ..................................................................................................................................... 31

 

Humidify the Incubator ...................................................................................................................................................... 33

 

Set the Temperature Setpoint ......................................................................................................................................... 34

 

Muting the Audible Temperature Alarm ....................................................................................................................... 35

 

Automatic Door Cutoffs ..................................................................................................................................................... 35

 

Start a Flow of CO

2

 ............................................................................................................................................................. 36

 

Set the CO

2

 Setpoint ........................................................................................................................................................... 37

 

Muting the Audible CO

2

 Alarm ........................................................................................................................................ 38

 

No Gas Supply Alarm (NGS) ............................................................................................................................................. 38

 

Set the Over Temperature Safety .................................................................................................................................. 39

 

Loading Samples ................................................................................................................................................................. 40

 

Accessory Compatibility .................................................................................................................................................... 40

 

Data Output Capabilities ................................................................................................................................................... 41

 

Condensation and the Dew Point .................................................................................................................................. 42

 

USER MAINTENANCE ............................................................................................................................................. 43

 

Cleaning and Disinfecting ................................................................................................................................................. 43

 

Minimizing Contamination Exposure ............................................................................................................................. 44

 

Maintaining Atmospheric Integrity ................................................................................................................................. 44

 

Gas Lines and HEPA Filters .............................................................................................................................................. 45

 

Electrical Components ....................................................................................................................................................... 45

 

Storing the Incubator .......................................................................................................................................................... 45

 

Replace the Chamber HEPA Filter ................................................................................................................................. 46

 

Calibrate the Temperature Display ................................................................................................................................. 47

 

Calibrate the CO

Display .................................................................................................................................................. 51

 

Summary of Contents for SCO10A

Page 1: ...AIR JACKETED CO2 INCUBATOR 110 120 Volts Installation Operation Manual SCO10A SCO5A...

Page 2: ...Advertencia Este producto contiene sustancias qu micas incluido el triglicidil isocianurato que el estado de California sabe que causa c ncer as como defectos de nacimiento u otros da os reproductivos...

Page 3: ...rs for professional industrial or educational use where the preparation or testing of materials is done at an ambient air pressure range of 22 14 31 3 inHg 75 106 kPa and no flammable volatile or comb...

Page 4: ...ion 21 Connect to the CO2 Supply 22 Access Port Stopper 23 GRAPHIC SYMBOLS 25 CONTROL PANEL OVERVIEW 27 OPERATION 29 Theory of Operation 29 Put the Incubator into Operation 31 Humidify the Incubator 3...

Page 5: ...5 P a g e UNIT SPECIFICATIONS 55 Weight 55 Dimensions 55 Max Shelves For Unit 56 Capacity 56 CO2 56 Temperature 56 Power 56 PARTS LIST 57 Accessories 58 Ordering 58...

Page 6: ...6 P a g e TABLE OF CONTENTS...

Page 7: ...w up a site specific standard operating procedure SOP covering each application and associated safety guidelines This SOP must be written and available to all end users in a language they understand I...

Page 8: ...ufacturing INC P O Box 627 Cornelius OR 97113 USA MANUFACTURING WARRANTY For information on your warranty and online warranty registration please visit sheldonmanufacturing com warranty ENGINEERING IM...

Page 9: ...ng contaminants into the chamber may damage the incubator and is not covered by the manufacturing defect warranty Supply Source and Pressure The incubator requires 15 20 psi of CO2 gas pressure at the...

Page 10: ...est CO2 accuracy use a digital gas analyzer with sample tubing connecting to the incubator CO2 sample port The barbed adapter gas fitting on the sample port connects to a 3 16 inch 4 76 mm inner diame...

Page 11: ...er service that delivered the unit 3 If the carton is not damaged open the carton and remove the contents 4 Inspect the unit for signs of damage Use the orientation images in this chapter as reference...

Page 12: ...rt Fuse in Power Inlet USB Style Port Control Panel CO2 Sample Port Side of Unit Chamber Gasket Ceiling Air Duct Chamber HEPA Filter Door Latch Humidification Pan Viewing Door Glass Chamber Door Chamb...

Page 13: ...13 P a g e RECEIVING Top Incubator SCO10A Mounting Bracket and SCO10 A Data Plate Bottom Incubator See the SCO5A depiction on the previous page for details on the individual incubators in the stack...

Page 14: ...this information to provide accurate help during support calls and emails SCO5A o The data plate is located on the inside of the incubation chamber door in the top right corner SCO10A o The data plat...

Page 15: ...able electrical outlet and power supply is present page 17 Install the incubator in a suitable location Review the lifting and handling instructions page 18 Install the unit leveling feet page 18 Inst...

Page 16: ...unit consider all environmental conditions that can adversely impact its temperature performance These include Proximity to ovens autoclaves and any device that produces significant radiant heat Heati...

Page 17: ...n 10 from the data plate rating Use a separate circuit to prevent loss of the unit due to overloading or circuit failure The recommended wall circuit breakers for these units are 15 amps The wall powe...

Page 18: ...r stabilization Restrain the unit completely while lifting or transporting so it cannot tip Remove all moving parts such as shelves and trays and lock doors in the closed position during transfers to...

Page 19: ...Ohm cm or a conductivity range of 20 0 uS cm to 1 0 uS cm for cleaning applications INSTALLATION CLEAN AND DISINFECT The manufacturer recommends cleaning the shelving ceiling air duct and chamber prio...

Page 20: ...eps to install the filter and ceiling air duct Snap the chamber HEPA filter to the mounting collar on the chamber ceiling air duct It may be necessary to tilt the filter to one side or the other Snap...

Page 21: ...Align the keyhole slot of the standard with the mounting peg on the side of the chamber wall Mount the shelf standard Install the shelf slides Insert the shelf slide into the shelf standard using a ro...

Page 22: ...upply source to each incubator The SCO10A requires a CO2 supply for each chamber Do not initiate a flow of CO2 to the incubator at this time End of Procedure CO2 to Chamber IN Connects to 3 16 4 76 mm...

Page 23: ...nside the chamber to obtain the best temperature uniformity and prevent condensation from forming inside the port o Do not install the stopper on the outside of the incubator Wires for thermocouples a...

Page 24: ...24 P a g e INSTALLATION...

Page 25: ...ichage de la temp rature Over Temperature Limit system Thermostat temp rature limite contr le haute AC Power Rep re le courant alternatif I ON O OFF I indique que l interrupteur est en position marche...

Page 26: ...26 P a g e SYMBOLS...

Page 27: ...ing to the incubation chamber by rerouting power away from the heating elements Set Temperature Display and Controls Shows the current chamber temperature The Up and Down arrow buttons are used to acc...

Page 28: ...to access the CO2 Setpoint SP or Calibration Offset C O display modes and input the CO2 setpoint or calibration adjustment value Red LED alarm indicators marked High and Low illuminate whenever the CO...

Page 29: ...l room it will increase the length of heating pulses to compensate Likewise when operating in a warm room the incubator uses shorter pulses If the ambient temperature conditions change significantly t...

Page 30: ...chamber The system operates independently of the main microprocessor temperature controller and routes power away from the incubator heating elements if the chamber temperature exceeds the OTL tempera...

Page 31: ...cord that came with the unit to the power inlet receptacle on the side of the incubator Plug the power cord into the workspace electrical supply outlet 3 Turn on the incubator Place the incubator Powe...

Page 32: ...t for at least 8 hours for example overnight with chamber doors closed prior to Setting the Over Temperature Limit next step Loading samples This helps ensure a stable temperature environment 9 Perfor...

Page 33: ...o slow the growth of microbiological populations in the humidification water supply Never use deionized water to humidify the incubator The pan must be placed on the chamber floor o The floor is heate...

Page 34: ...temperature setpoint adjustment mode The display will briefly flash the letters SP then show the flashing adjustable temperature setpoint Note The display will automatically exit the adjustment mode a...

Page 35: ...r the duration of the current temperature deviation The visual alarm indicator will remain illuminated Any new deviation of 1 C or greater will reactivate the audible alarm AUTOMATIC DOOR CUTOFFS Gas...

Page 36: ...ply CO2 to the regulator Set the cylinder regulator flow setting Set the regulator to deliver 15 20 Pounds per Square Inch psi to the incubator CO2 in Port Never exceed 20 psi Building Supply System I...

Page 37: ...buttons to activate the CO2 setpoint adjustment mode The display will briefly flash the letters SP then show the flashing adjustable CO2 setpoint Note The display will automatically exit the adjustmen...

Page 38: ...off The audible alarm will remain muted for the duration of the current CO2 deviation The visual alarm indicator will remain illuminated Any new deviation of 1 or greater will reactivate the audible a...

Page 39: ...activating check for ambient sources of heat or cold that may be adversely impacting the unit temperature stability If you find no sources of external or internal temperature fluctuations contact Cust...

Page 40: ...r spacing allows for maximum air circulation and a higher degree of temperature uniformity Proper spacing also decreases the chance of condensate forming in the incubator when operating with a large n...

Page 41: ...Outputs The temperature and CO2 levels are also outputted as analog signals by an internal 4 20 milliamp board The outputs are transmitted through two jack ports on the back of each incubator unit Th...

Page 42: ...y in the incubation chamber is already near the dew point barometric fluctuations may push it across the dew point threshold Note Thin air at higher altitudes holds less humidity than the denser air f...

Page 43: ...iner Do not clean or disinfect the ring style chamber HEPA filter Replace the filter if discolored or if you believe it has been contaminated Warning Exercise caution if cleaning the unit with alcohol...

Page 44: ...rom evaporating disinfecting agents can inhibit growth or cause metabolic symptoms in microbiological sample populations Make sure that chlorines quaternary ammonias or any other overtly volatile disi...

Page 45: ...ss permanent kinking or other signs of damage are present ELECTRICAL COMPONENTS Electrical components do not require maintenance If the incubator fails to operate as specified please contact your dist...

Page 46: ...er the front of the duct This safeguards against accidentally striking the blower fan and sensor heads with the duct Pull the duct and attached HEPA filter out of the incubation chamber Replacing the...

Page 47: ...must be humidified for at least 24 hours in order to conduct an accurate calibration A Suggested Calibration Set Up CO2 Calibration A CO2 calibration may be performed during the temperature calibrati...

Page 48: ...Calibration 1 Once the incubator temperature has stabilized compare the reference device and incubator temperature display readings If the readings are the same or the difference between the two fall...

Page 49: ...saved 4 Use the Up or Down arrows to adjust the current display temperature value until it matches the reference device temperature reading Reference Device 5 After matching the display to the referen...

Page 50: ...ubator is now calibrated for temperature OR See the next step if the readings fail to match or fall outside of your protocol range Reference Device 8 If the difference still falls outside the acceptab...

Page 51: ...ten as required by your laboratory or production protocol or regulatory compliance schedule Always calibrate to the standards and use the calibration setup required by your industry requirements or la...

Page 52: ...ested CO2 Calibration 1 Once the incubator CO2 concentration has stabilized compare the reference device and the CO2 display readings If the readings are the same or the difference between the two fal...

Page 53: ...Down arrow buttons to adjust the current display CO2 value until it matches the reference device concentration reading Reference Device 5 After matching the display to the reference device wait 5 seco...

Page 54: ...or is now calibrated for CO2 OR See the next step if the readings fail to match or fall outside of your protocol range Reference Device 8 If the difference still falls outside the acceptable range of...

Page 55: ...inner chamber All indications are average values typical for units produced in the series We reserve the right to alter technical specifications at all times WEIGHT Model Shipping Unit SCO5A 238 lb 10...

Page 56: ...0 142 0 SCO10A 5 0 142 0 Each Chamber CO2 Model Range Accuracy Recovery Time All 0 20 0 1 Less than 5 minutes TEMPERATURE Model Range Uniformity Stability All Ambient 5 C to 60 C 0 25 C at 37 C 0 1 C...

Page 57: ...cation Pan 995 00015 Chamber HEPA Filter Cap 6500506 Leveling Foot 2700512 Ceiling Air Duct Chamber 5121527 Power Cord 115 volt 15 Amp 9ft 5 in 2 86m NEMA 5 15P 1800510 CO2 Gas Tubing Kit with In Line...

Page 58: ...ssembly described above PN 5820504 Individual Shelf PN 5820505 Pair of Slides ORDERING Accessories and replacement parts can be ordered online at parts sheldonmfg com If the required item is not liste...

Page 59: ...59 P a g e PARTS...

Page 60: ...P O Box 627 Cornelius OR 97113 USA support sheldonmfg com sheldonmanufacturing com 1 800 322 4897 503 640 3000 FAX 503 640 1366...

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