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P a g e
INTRODUCTION
R
EFERENCE
S
ENSOR
D
EVICES
Must be purchased separately
Reference sensor devices or a combined device are required for calibrating the
incubator temperature and CO
2
displays.
Reference devices must meet the following standards:
•
Accurate to at least 0.1°C
•
Accurate to at least 0.1% CO
2
The devices should be regularly calibrated, preferably by a third party.
Temperature Probes
For temperature, use a digital device with wire thermocouple probes that can be
introduced into the incubation chamber through the unit access port. Select
thermocouples suitable for the application temperature you will be calibrating at.
CO
2
Sampling
For best CO
2
accuracy, use a digital gas analyzer with sample tubing connecting to
the incubator CO
2
sample port. The barbed adapter gas fitting on the sample port
connects to
a 3/16-inch (4.76 mm)
inner diameter (ID), 5/16-inch (7.94 mm) outside
diameter (OD) tubing.
Why Probes and Tubing?
Reference readings taken outside the chamber using wire temperature probes and
CO
2
drawn through the sample port avoid chamber door openings. Openings disrupt
the chamber temperature and CO
2
concentration level. Each disruption requires
a
minimum 1-hour wait
to allow the atmosphere to re-stabilize before continuing.
No Alcohol or Mercury Thermometers
Alcohol thermometers do not have sufficient accuracy to conduct accurate
temperature calibrations.
Never place a mercury thermometer in the incubation
chamber.
Always use thermocouple probes.
CO
2
Reference
Temperature
Reference
