Sequoia LISST-HOLO, User Manual

Page: 34 / 73

34 LISST-
HOLO User’s Guide
12 Focus Depth (from emit window) mm
13 Bounding Box Pixels
14 Orientation Degrees
15 Euler Number Dimensionless
16 Extent Dimensionless
17 Perimeter
μm
.TIFF files of individual slices
If the check box ‘Save Individual Slices’ was checked, HOLO_Batch will save a .TIFF image for each reconstructed slice,
showing the in focus and out of focus particles that appear on that particular slice.
The distance from the transmit window of each slice is given
in the image filename. For example the image on the left is
016-0270-z22.500.TIFF, meaning it was reconstructed from
hologram 016-0270.pgm at a distance of 22.5 mm from the
transmit window.
Summary of files created by HOLO_Batch
Check Box Checked?
2
The Centroid X, Y locations specify where the particle is located. Together with the Focus Depth, these 3 numbers locate the particle in the 3-D
space of the laser beam.