Schambeck SFD GmbH
Refractive Index Detector RI2012
RI2012 V1.0 06-2013.EN / 28.10.2013
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http://www.schambeck-sfd.com
Now the signal at the integrator output and the recorder output is exactly 0 mV. Press the
ENTER
button again to switch to the next voltage. The current voltage which is applied to
the signal output is shown in the display.
15.4 C
ALIBRATING THE
RI2012
The calibration mode of the RI2012 detector is used to calibrate the signal response to a
certain sample concentration. This step is needed if the instrument is used to perform
quantitative analysis. The detector is calibrated when shipped to the customer. Usually a re-
calibration has to be performed after modifications of the optical system only. Under normal
operation conditions a re-calibration of the RI2012 is not needed.
To calibrate the signal response of the instrument you need a solution of exactly
106 mg glucose in 100 mL distilled water
which is used as test solution. The refractive
index of this solution is known and saved as an internal constant in the detectors firmware.
The first step of the calibration is purging the reference chamber and the sample chamber of
the flow cell with distilled water. In the second step the sample chamber is filled with the
standard solution. At this point the theoretical detector signal is 500 mV. The real detector
signal is recognized by the firmware and the calibration factor to reach the theoretical
500 mV is calculated and saved as internal constant.
The correlation between the refractive index and the detector signal is:
1 mV
1 µRIU
