Schambeck SFD GmbH
Refractive Index Detector RI2012
RI2012 V1.0 06-2013.EN / 28.10.2013
21
http://www.schambeck-sfd.com
13.2 P
URGE
M
ODE WITH
ASCO
V
ALVE
The purge mode is used to flush the reference chamber or the flow cell with fresh mobile
phase (solvent). If the purge mode is activated the red LED will light up. The mobile phase
now passes the reference chamber of the flow cell.
After leaving the column the phase enters the detector passing the INLET port. After this the
mobile phase enters the optical bench passing the heat exchanger going towards the flow
cell. Now the stream enters the sample chamber of the flow cell. Leaving the flow cell,
passing the heat exchanger again the liquid reaches the T-connector and after this it
reaches the flow cell. Now the reference chamber is flushed by mobile phase. Leaving the
flow cell the liquid is guided through the OUTLET port out of the detector.
The purge mode should be activated regularly to ensure that the reference chamber
contains mobile phase (solvent) which is similar to the mobile phase passing the sample
chamber.
After purging the detector for a certain time you can start your measurement after
deactivation of the purge mode by pressing the
PURGE
button once again.
The following figure illustrates the liquid stream in purge mode with ASCO - valve.
The purge mode should be activated until a stable baseline is reached. After switching back
to the measuring mode it might be necessary to wait once more until the baseline becomes
stable.
