5.2.2 Inoculation
For information regarding use of the intuitive Touch screen, please refer to the
associated manual for BIOSTAT
®
RM Control Tower.
1. Inoculate the contents of the syringe via the Luer-Septum into the
CultiBag RM 600.
2. Disinfect the Luer-Septum again and close it with the protective cap.
3. Drain back the liquid in the tubing.
Pinch the sampling connector tubing a few times to ensure that any liquid
in the tubing drains back into the bioreactor. Close the tubing clamp.
4. Restart the Rocking Motion
Adjust the speed to get a visible wave on the surface of the liquid.
Reduce the speed if you see excessive foaming.
Inoculation can also be done by any other port of the CultiBag RM 600.
Luer couplings and MPC couplings with their surfaces in contact with atmosphere
have to connected in a laminar flow.
Use of BioWelder or BioSealer allows simple, fast and reliable connections .
5.2.3 Sampling
The procedure is the same as that described for inoculation using a needle less
syringe.
The Luer septum is proven for 50 sterile samplings or inoculations. The connector
contains a silicone septum, which automatically seals the device whenever it is
disconnected.
The sample is also completely contained as no aerosols are vented. At low rocking
rates (< 8 rocks/min.) the cells might settle in the media, which does not provide
a representative sample. In this situation, the recommended technique is to raise
the rocking rate more than 8 rocks/min., 5 up to 10 minutes prior to sampling.
The rocking speed can be decreased after sampling.
The sampling device has a fairly small orifice. If you are working with large
microcarriers or large cell aggregates do not use the sampling connector.
26
End of the process and putting out of service safely