5.1.2 Set cultivation parameters and start
For information regarding use of the intuitive Touch screen, please refer to the
associated manual for BIOSTAT
®
RM Control Tower.
1. Adjust required aeration rate. Start aeration if aeration is not already activated.
For minimum and maximum aeration rate see section Aeration control and
Aeration Flow rate.
When using low or middle gassing rate (below 20 lpm), we recommend activating
the pressure control loop to hold a constant middle pressure in the bag.
When using high gassing rate (20 lpm or higher) the pressure control can be
deactivated, because the gas outlet filter and the proportional will produce a
back pressure in the bag.
We recommend starting the aeration process parameter first, so that the gas
outlet filters are preheated.
2. Adjust the rocking rate and rocking angle. A visible wave on the surface of the
liquid is required. Reduce the speed if you see excessive foaming. Make sure
that the bioreactor chamber is rigidly inflated; otherwise excessive foam will be
generated from turbulence caused by creases in the bag.
Start rocking motion.
For preliminary estimation of rocking rate and rocking angle, see the section
Optimize Wave Motion.
For minimum and maximum rocking rate see section Rocking rate, for minimum
and maximum rocking angle see section Rocking angle.
Always close the lid while rocking motion is activated.
Never put any objects on cover of rocking table while rocking motion is activated.
Avoid placing hands in the danger area of moving parts.
3. Adjust required set temperature.
For minimum and maximum temperature see section Temperature control.
Do not start heating unless the surface temperature sensor is correctly positioned
under the CultiBag RM. Otherwise over heating may occur.
Note that, if the rocking motion is started before you activate the heater, this will
guarantee uniform temperature distribution in the medium.
24
Cultivation
NOTICE