4.6
Connect Optical Sensors
Observe the colour markers at the device, the aluminium clamp and the markers
at the ends of the light fibre cables.
The pH has a yellow sticker, the DO has a red sticker. Do not mix up the light fibre
cables.
Both ports are marked with a label with the inscriptions „pH“ and „DO“. Put the
clamps on both ports of the pH und DO measurement.
The colours of the label have to comply with the colour of the respective light fibre
cable i.e. red to red for DO and yellow to yellow for pH.
1. CultiBag RM 600 has to be inflated. It will not be possible to insert the optical
fibre cables into the sensors ports of a CultiBag RM 600 that is not inflated.
Put the clamps on both ports of the pH und DO measurement.
2. Insert the optical fibre cables into the sensors ports on the bag.
Insert the optical fibre cable of DO measurement into port with red label
“OptiSens DO.
Insert the optical fibre cable of pH measurement into port with yellow label
“OptiSens pH”.
3. The optical fibre cables are inserted until they touch the inner surface of the
sensor caps. If there is no contact between the light fibre cable and the sensor
cap no signal will be measured. Tighten the screws of the clamps to fix the
cables in place.
4. For accuracy and for validated processes, the pH should be calibrated with
an additional reference measurement. A sample of the media has to be taken
asceptically from the bag and the offline pH and DO should be measured.
22
Cultivation