5.2
Sampling and Inoculation
This operation requires a standard luer type syringe, whose luer septum is without
hypodermic needle. It is not necessary to perform this procedure in a laminar flow
cabinet.
5.2.1 Preparing for Sampling or Inoculation
For information regarding use of the intuitive Touch screen, please refer to the
associated manual for BIOSTAT
®
RM Control Tower.
1. Continue headspace aeration.
2. Move the front side of rocking table down by adjusting the angle to keep
the dip tube of the sample port in the media.
Set angle in menu.
For minimum and maximum of adjustable angle see section Rocking angle.
Do not open lid of rocking table.
3. Remove the protective cap from the luer septum sampling connector.
4. Disinfect the septum: wipe or spray the top of the Luer septum with 70% alcohol
(or equivalent).
5. Connect the syringe: Using aseptic technique, attach a sterile disposable
needleless syringe onto the luer connector.
6. Open the hose clamp of the luer septum. Draw the sample, close the hose clamp
of the luer septum and the remove the syringe carefully.
Cultivation
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