5010e_513.doc / 22.01.10
5.4.10 Calculation procedure 10 (FTK/S/Rb)
Method at which a reagent blank is measured after an incubation time (
⇒
A
RB,0
) and after a reaction time (
⇒
A
RB,1
) and also a sample after an incubation time (
⇒
A
S,0
) and after a reaction time (
⇒
A
S,1
).
The difference from the change of the sample and the change of the reagent blank becomes multiplied with a
factor F which is determined by means of the change of standard solution |A
ST,0
-A
ST,1
| and the change of
reagent blank |A
RB,0
-A
RB,1
| during the reaction time and given concentration of standard. The reagent blank A
RB
is entered or measured once.
During the procedure the dialog asks for the use of a reagent blank. The default value is OFF. To continue with-
out reagent blank press [ENTER].
After each measurement the next sample can be measured with [NEXT].
With [RESULT] it is possible to measure the same sample again.
Calculation procedure .....................................................CP 10
Characteristic ........................................................FTK / S / Rb
Method ............................................. Fixed Time with Standard
Calculation formula ......... C = F
∗
( |A
S,0
- A
S,1
| - |A
RB,0
- A
RB,1
| )
Resulting factor ..............F = C
ST
/ ( |A
ST,0
-A
ST,1
| - |A
RB,0
-A
RB,1
| )
Reagent blank ........................................entering or measuring
PHOTOMETER 5010 #7000
V5.13a 02/09/09 D
LAB.: RIELE GMBH+CO KG
DATE: 09/08/09
TIME: 10:03:16
METHOD 30: CREATININ
PROGRAM: 10
STANDARD: 2.00
WAVELENGTH: 492nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
INCUBATION: 45s
REACTION: 60s
MAX.UNITS: 25
UNIT: mg/dl
- - - - - - - - - - - -
MEASURE BLANK
Rb[A]: 0.000
ST/KIN 1: 0.194
ST/KIN 2: 0.203
ST/KIN 3: 0.214
ST/KIN: 0.204
FACTOR: 9.80
NO. ABS. RESULT
- - - - - - - - - - - -
1 0.326 9.84
DELTA [A]: 1.005
- - - - - - - - - - - -
2 0.336 10.81
DELTA [A]: 1.103
- - - - - - - - - - - -
3 0.329 12.84
DELTA [A]: 1.310
Start method selection in the main menu.
See chapter:
4.1 - Measurement with programmed methods
4.2 - Measurement with basic methods
In the case of activated printer the print-out of the method data
follows.
The measuring window is shown.
Method procedure:
Æ
Insert / measure zero solution
Without reagent blank (insert / measure optionally)
Æ
Insert / measure standard 1
Æ
Insert / measure standard 2 (optional)
Æ
Insert / measure standard 3 (optional)
(Averaged standard)
(Resulting factor)
Æ
Insert / measure sample
Æ
Insert / measure sample
Æ
Insert / measure sample
ROBERT RIELE GmbH & Co KG
Page 33
5 - CALCULATION PROCEDURES
