5010e_513.doc / 22.01.10
5.4.6
Calculation procedure 6 (C/S/Rb)
Method at which the difference of sample value A
S
and reagent blank A
RB
is multiplied with a factor F which is
determined by measuring of a standard solution with known concentration C
ST
and under consideration of
reagent blank A
RB
.
The reagent blank A
RB
is entered or measured once.
Calculation procedure .......................................................CP 6
Characteristic ............................................................ C / S / Rb
Method ............................................... End Point with Standard
Calculation formula .......................................C = F
∗
(A
S
- A
RB
)
Resulting factor ........................................... F = C
ST
/ (A
ST
-A
RB
)
Reagent blank ........................................entering or measuring
PHOTOMETER 5010 #7000
V5.13a 02/09/09 D
LAB.: RIELE GMBH+CO KG
DATE: 09/08/09
TIME: 08:45:09
METHOD 26: SODIUM
PROGRAM: 6
STANDARD: 150.0
WAVELENGTH: 405nm
TEMPERATURE: 37C
MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul
DELAY: 3s
MAX.UNITS: 300
UNIT: mmol/l
- - - - - - - - - - - -
MEASURE BLANK
Rb[A]: 0.108
ST[A] 1: 1.112
ST[A] 2: 1.132
ST[A] 3: 1.118
ST[A]: 1.121
FACTOR: 148.2
NO. ABS. RESULT
- - - - - - - - - - - -
1 1.449 198.7
- - - - - - - - - - - -
2 1.118 149.6
- - - - - - - - - - - -
5 2.006 281.2
Start method selection in the main menu.
See chapter:
4.1 - Measurement with programmed methods
4.2 - Measurement with basic methods
In the case of activated printer the print-out of the method data
follows.
The measuring window is shown.
Method procedure:
Æ
Insert / measure zero solution
Æ
Insert / measure reagent blank
Æ
Insert / measure standard 1
Æ
Insert / measure standard 2 (optional)
Æ
Insert / measure standard 3 (optional)
(Averaged standard)
(Resulting factor)
Æ
Insert / measure sample
Æ
Insert / measure sample
Æ
Insert / measure sample
ROBERT RIELE GmbH & Co KG
Page 29
5 - CALCULATION PROCEDURES
