Riele Photometer 5010 V5+ Operating Instructions Manual Download Page 31 | Manualshive

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5.4.8

Calculation procedure 8 (C/S/SbRb)

Method at which the difference of reagent blank A

RB

and blank of reagent blank A

RBB

regarding the amount is

subtracted of the difference of sample value A

S

and sample blank A

SB

regarding the amount and this difference

is multiplied with a factor F which is determined by measuring of a standard solution with known concentration
C

ST

and under consideration of standard blank A

STB

and the difference of reagent blank A

RB

and blank of

reagent blank A

RBB

The sample blank A

SB

is measured before every test. The reagent blank A

RB

is entered or measured once.

Calculation procedure .......................................................CP 8
Characteristic ........................................................C / S / SbRb
Method ............................................... End Point with Standard
Calculation formula ..............C = F

∗

( |A

S

- A

SB

| - |A

RB

- A

RBB

| )

Resulting factor ...................F = C

ST

/ ( |A

ST

-A

STB

| - |A

RB

-A

RBB

| )

Reagent blank ........................................entering or measuring

PHOTOMETER 5010 #7000
V5.13a 02/09/09 D

LAB.:   RIELE GMBH+CO KG
DATE:           09/08/09
ZEIT:           09:33:26

METHOD 28: Ca
PROGRAM: 8

STANDARD:           8.02
WAVELENGTH:       546nm
TEMPERATURE:         37C

MEAS. VOLUME: 900ul
WASH VOLUME: 1000ul

DELAY:               3s
MAX.UNITS:           12
UNIT:              mg/dl

- - - - - - - - - - - -
MEASURE BLANK

Rb[A]: 0.150
Rbb[A]: 0.046

DELTA Rb: 0.104

ST[A] 1: 1.485

ST[A] 2: 1.521
ST[A] 3: 1.495

ST[A]: 1.501
STb[A]: 0.479

DELTA ST: 1.022
FACTOR: 8.74

NO. ABS. RESULT
1 1.495 7.89

Sb[A]: 0.489
- - - - - - - - - - - -
2 1.542 7.89

Sb[A]: 0.535
- - - - - - - - - - - -

3 1.394 8.39
Sb[A]: 0.329
- - - - - - - - - - - -

Start method selection in the main menu.
See chapter:
4.1 - Measurement with programmed methods
4.2 - Measurement with basic methods

In the case of activated printer the print-out of the method data
follows.

The measuring window is shown.

Method procedure:

Æ

Insert / measure zero solution

Æ

Insert / measure blank of reagent blank

Æ

Insert / measure reagent blank

(Resulting blank)

Æ

Insert / measure standard blank

Æ

Insert / measure standard 1

Æ

Insert / measure standard 2 (optional)

Æ

Insert / measure standard 3 (optional)

(Averaged standard)
(standard blank)
(Averaged standard minus standard blank)
(Resulting factor)

Æ

Insert / measure sample blank

Æ

Insert / measure sample

Æ

Insert / measure sample blank

Æ

Insert / measure sample

Æ

Insert / measure sample blank

Æ

Insert / measure sample

ROBERT RIELE GmbH & Co KG

Page 31

5 - CALCULATION PROCEDURES

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Summary of Contents for Photometer 5010 V5+

Page 1: ...Operating Instructions Photometer 5010 V5 ROBERT RIELE GmbH Co KG Software Version 5 13 Documentation Version 01 2010 ...

Page 2: ...n origin must be treated as potentially infectious The relevant laboratory guidelines on safe use must be observed Symbol for the marking of electrical and electronics devices according to 7 ElektroG IP XO No special protection against penetrating moisture IP International Protection REF Order number SN Serial number INSTRUMENT APPROVALS The Photometer 5010 meets the requirement stated in Directiv...

Page 3: ...zed specialist staff Through improper repairs the warranty extinguishes and the operator can be heavily jeopard ized If suspected the device can no longer be operated safely turn it off and take steps to ensure that no one will subsequently attempt to use it Electromagnetic waves Devices that emit electromagnetic waves may affect measured data or cause the Photometer 5010 to malfunc tion Do not op...

Page 4: ...osts for an environmental waste disposal The previous professional decontamination has to be proved with a certificate Address of the manufacturer ROBERT RIELE GmbH Co KG Kurfuerstenstrasse 75 79 13467 BERLIN GERMANY Phone 49 0 30 404 40 87 Fax 49 0 30 404 05 29 E mail info riele de www riele de QUALITY MANAGEMENT SYSTEM ROBERT RIELE GmbH Co KG maintains a quality management system according to EN...

Page 5: ...andard 18 5 1 5 Fundamental to the methods with multi standards 18 5 1 6 Fundamental to bichromatic measurements 18 5 1 7 Fundamental to the Kinetic 19 5 1 8 Fundamental to the methods with reagent blank 20 5 1 9 Fundamental to ID NO and sample numerator 20 5 1 10 Fundamental to storing test results 20 5 2 ABBREVIATIONS 21 5 3 SURVEY OF THE METHODS 22 5 4 DESCRIPTION OF METHOD PROCEDURES 23 5 4 1 ...

Page 6: ... 19 Send results 61 7 2 20 Service tools 61 8 MAINTENANCE 62 8 1 CLEANING INSTRUCTION 62 8 2 CALIBRATING MEASURING SYSTEM 62 8 3 ADJUSTMENT OF BUBBLE DETECTOR 62 8 4 CALIBRATION OF PERISTALTIC PUMP 62 8 5 REPLACEMENT OF RIBBON 62 8 6 REPLACEMENT OF PAPER ROLL 63 8 7 REPLACEMENT OF ASPIRATION TUBE 63 8 8 REPLACEMENT OF LINE FUSES 63 9 ERROR MESSAGE CORRECTION 64 9 1 GENERAL NOTE 64 9 2 ACOUSTIC ERR...

Page 7: ...rovided standard cuvette adaptor By means of a combination of transistor and Peltier element the solution reaches fast and accurately one of the three selectable temperatures 25 C 30 C or 37 C The Photometer 5010 is standard equipped with six optical filters of the wavelengths 340 405 492 546 578 and 623 nm If required they can be exchanged against any wavelength within the range of 340 800 nm Thr...

Page 8: ...ize suffi ciently before operation Before connecting the waste tube to the pump tube remove pump tube at both ends from the metal clamps The waste tube of the flow through system must be led through the tunnel to the backside of the device chapter 3 2 BACK and then into any drain tank 2 3 INSTALLATION Photometer 5010 operates at any line voltage between 90 VAC and 264 VAC at 50 60 Hz The device pl...

Page 9: ...8 0 9 1 1 3 9 If errors appeared during operation first of all they have to be confirmed with E before remedy chapter 9 ERROR MESSAGE CORRECTION 2 4 LOADING PRINTER PAPER With initial operation or if the colored end of the paper roll appears printer paper must be inserted Open the printer cover and remove the rest of paper Put printer paper axis into the new printer paper reel Fold the beginning o...

Page 10: ...5010e_513 doc 22 01 10 OPERATING ELEMENTS 3 OPERATING ELEMENTS 3 1 FRONT working area printer cover lid aspiration tube touchscreen sipping lever P 3 2 BACK ROBERT RIELE GmbH Co KG Page 10 3 ...

Page 11: ...he desired range on the screen must be touched The surface of the touchscreen may be never touched with ball point pen pencil or another pointed article 3 4 WORKING AREA pump tube with joints REF 5010 050 outlet tube cuvette REF 5010 066 waste tubing REF 1704834001 aspiration tubing REF 5010 065 joint inlet tube cuvette REF 1707175001 ROBERT RIELE GmbH Co KG Page 11 3 OPERATING ELEMENTS ...

Page 12: ...ea It must sit as close as possible at the connection of the flow through cuvette For working without bubble detector switch off this function chapter 7 2 4 3 Bubble detector ON OFF While working with the flow through system the tubes must not be sharply bended No residues should be inside Check from time to time that the tubing and the connections are leakproof After each replacement of tubing ex...

Page 13: ...rough cuvette insert the standard cuvette adaptor The path of rays is directed from the back to the front of the device Insert flow through cuvette according to the drawing OPTIC CONSTRUCTION in TECHNICAL DATA Pay attention to the correct connection of aspiration tube and outlet tube cuvette Switch on the pump by the function Pump ON OFF chapter 7 2 4 1 Switch on or off bubble detector by the func...

Page 14: ...37 03C In the case of stable temperature the current temperature of e g 37 01C is shown Small fluctuations of the value are normal Date in the format day month year Time M A I N M E N U M E A S U R E W I T H P R O G R M E T H O D S M E A S U R E W I T H B A S I C M E T H O D S M E T H O D S N E W C H A N G E C O P Y U T I L I T I E S L A M P L F 3 7 0 5 C 0 9 0 8 0 9 1 1 3 9 4 1 Measurement with p...

Page 15: ...d for the distinction of the methods CONC concentration KIN kinetic F factor STD standard RB reagent blank SB sample blank Further information Chapter 5 CALCULATION PROCEDURES B A S I C M E T H O D S P A G E 1 4 C O N C W F A C T O R P A G E C O N C W F A C T O R R B C O N C W F A C T O R S B C O N C W F A C T O R R B S B E X I T 4 3 Method editor Each photometric test can be permanently stored wi...

Page 16: ...zero solution and pressing ZERO 4 6 Line feed LF Pressing LF in the main menu triggers a line feed in the case of activated printer Several lines can be advanced by continuous pressure on LF U T I L I T I E S P A G E 1 5 D A R K A D J U S T M E N T P A G E M U L T I S T A N D A R D P R I N T E R P U M P B U B B L E D E T E C T E X I T 3 7 0 5 C 0 9 0 8 0 9 1 1 3 9 3 7 0 5 C 0 9 0 8 0 9 1 1 3 9 3 7...

Page 17: ... C is reached The current temperature of the flow through cuvette or of the cuvette adaptor is shown at the Iower edge of the touchscreen For meaning of the display see chapter 4 PROGRAM SELECTION MAIN MENU A temperature instable or out of tolerance during measuring is marked by an asterisk at the utmost right position in the corresponding print line To avoid deviations due to temperature influenc...

Page 18: ...nding method number After renewed selection of this method the last resulting factor is offered as OLD STD The principle of the multiple measurement can also be expanded to all measurements The corresponding entry can be set invoking a basic method The parameter is definable in prepro grammed methods chapter 6 METHOD EDITOR 5 1 5 Fundamental to the methods with multi standards Linear calibration i...

Page 19: ...ute non linearity Already values 0 9 indicate a bad linearity and therefore an incorrect test In practice linear tests show values of R 2 near to 1 In the example for Calculation procedure 11 KIN F Rb values of R 2 0 998 are permitted Results with smaller R 2 values could be caused by temperature instability pollution expired reagents unfavorable delay time etc For a better monitoring the number o...

Page 20: ... sample numerator All test results are labeled with a numerator Additionally all results can be labeled with a 5 digit ID NO When the ID NO is not zero it will be displayed and printed together with the sample result When a method is selected you can edit both numerator and ID NO of a sample with MODE NUM Press N I to switch between editing numerator and ID NO N E W S A M P L E N O 1 9 9 1 2 3 4 5...

Page 21: ...entration CST Concentration of standard CV Quality control Coefficient of variation dA min At Kinetic A min ARB Minit At Kinetic change of reagent blank per minute measured in A min AS Minit At Kinetic change of sample per minute measured in A min F Factor FTK Fixed Time Kinetic KIN Kinetic n Quality control number of values nm Nanometer dimension of wavelength m Quality control mean of values R R...

Page 22: ...P 6 C S Rb Endpoint with Standard C F AS ARB CP 7 C S Sb Endpoint with Standard C F AS ASB CP 8 C S SbRb Endpoint with Standard C F AS ASB ARB ARBB CP 9 FTK F Rb Fixed Time Kinetic with Factor C F AS 0 AS 1 ARB 0 ARB 1 CP 10 FTK S Rb Fixed Time Kinetic with Standard C F AS 0 AS 1 ARB 0 ARB 1 CP 11 KIN F Rb Kinetic with Factor C F AS Minit ARB Minit CP 12 KIN S Rb Kinetic with Standard C F AS Minit...

Page 23: ...ion name of active method reading window numerator ID number dimension action keys info line 16 5 Additional method specific information Functions of the action keys in the measuring window EXIT Leads to the query whether the measuring program is to be terminated MODE Occupies the action keys with following mode functions NUM MODE LF QC RETURN PRN DETAIL LAMP M STD RETURN WASH With activated pump ...

Page 24: ...MOGLOBIN PROGRAM 1 FACTOR 29 4 WAVELENGTH 405nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 5s MAX UNITS 25 UNIT g l MEASURE BLANK NO ABS RESULT 1 0 675 19 8 2 0 843 24 8 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the print out of the method data follows The measuring...

Page 25: ...08 09 TIME 08 52 24 METHOD 21 HDL C PROGRAM 2 FACTOR 325 WAVELENGTH 546nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 5s UNIT mg dl MEASURE BLANK Rb A 0 058 NO ABS RESULT 1 1 064 327 2 1 188 367 3 1 340 417 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the print out of t...

Page 26: ... 80 WAVELENGTH 546nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 5s MAX UNITS 8 0 UNIT mg dl MEASURE BLANK NO ABS RESULT 1 1 000 4 21 Sb A 0 671 2 1 215 4 25 Sb A 0 884 3 1 033 4 23 Sb A 0 702 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the print out of the method data...

Page 27: ...LAB RIELE GMBH CO KG DATE 09 08 09 TIME 10 11 15 METHOD 24 Fe PROGRAM 4 FACTOR 1330 WAVELENGTH 578nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 5s MIN UNITS 37 MAX UNITS 158 UNIT ug dl MEASURE BLANK Rb A 0 085 Rbb A 0 198 DELTA Rb 0 113 NO ABS RESULT 1 0 715 154 Sb A 0 486 2 0 646 49 Sb A 0 497 Start method selection in the main menu See chapter 4 1 Measurement with programmed meth...

Page 28: ...900ul WASH VOLUME 1000ul DELAY 3s MAX UNITS 22 2 UNIT mmol l MEASURE BLANK ST A 1 1 110 ST A 2 1 093 ST A 3 1 059 ST A 1 088 FACTOR 5 10 NO ABS RESULT 1 1 026 5 23 2 1 357 6 92 3 1 582 8 07 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the print out of the method data follows The measu...

Page 29: ... 6 STANDARD 150 0 WAVELENGTH 405nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 3s MAX UNITS 300 UNIT mmol l MEASURE BLANK Rb A 0 108 ST A 1 1 112 ST A 2 1 132 ST A 3 1 118 ST A 1 121 FACTOR 148 2 NO ABS RESULT 1 1 449 198 7 2 1 118 149 6 5 2 006 281 2 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the...

Page 30: ... UNITS 220 UNIT mg dl MEASURE BLANK ST A 1 0 614 ST A 2 0 629 ST A 3 0 620 ST A 0 621 STb A 0 106 DELTA ST 0 515 FACTOR 97 1 NO ABS RESULT 1 2 292 197 6 Sb A 0 257 2 2 340 198 0 Sb A 0 300 3 2 223 197 2 Sb A 0 193 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the print out of the metho...

Page 31: ...RD 8 02 WAVELENGTH 546nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 3s MAX UNITS 12 UNIT mg dl MEASURE BLANK Rb A 0 150 Rbb A 0 046 DELTA Rb 0 104 ST A 1 1 485 ST A 2 1 521 ST A 3 1 495 ST A 1 501 STb A 0 479 DELTA ST 1 022 FACTOR 8 74 NO ABS RESULT 1 1 495 7 89 Sb A 0 489 2 1 542 7 89 Sb A 0 535 3 1 394 8 39 Sb A 0 329 Start method selection in the main menu See chapter 4 1 Measur...

Page 32: ...ime with Factor Calculation formula C F AS 0 AS 1 ARB 0 ARB 1 Factor given entering Reagent blank entering or measuring PHOTOMETER 5010 7000 V5 13a 02 09 09 D LAB RIELE GMBH CO KG DATE 09 08 09 TIME 09 47 33 METHOD 29 CK MB PROGRAM 9 FACTOR 2751 3 WAVELENGTH 340nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul INCUBATION 120s REACTION 180s MAX UNITS 1500 UNIT U l MEASURE BLANK Rb A 0 000 NO ...

Page 33: ...tion formula C F AS 0 AS 1 ARB 0 ARB 1 Resulting factor F CST AST 0 AST 1 ARB 0 ARB 1 Reagent blank entering or measuring PHOTOMETER 5010 7000 V5 13a 02 09 09 D LAB RIELE GMBH CO KG DATE 09 08 09 TIME 10 03 16 METHOD 30 CREATININ PROGRAM 10 STANDARD 2 00 WAVELENGTH 492nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul INCUBATION 45s REACTION 60s MAX UNITS 25 UNIT mg dl MEASURE BLANK Rb A 0 00...

Page 34: ... 11 FACTOR 1746 0 WAVELENGTH 340nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 60s DELTAS 5 TIME DELTA 18s MAX UNITS 280 MIN R 2 0 998 UNIT U l MEASURE BLANK Rb A 0 000 NO ABS RESULT 1 0 123 189 7 R 2 0 9996 2 0 154 189 9 R 2 0 9993 3 0 110 158 5 R 2 0 9986 ABS 1 0 812 ABS 2 0 702 1 0 0867 2 0 0899 3 0 0871 4 0 0874 5 0 1087 DELTA REG 0 0908 Start method selection in the main menu S...

Page 35: ...OGRAM 12 STANDARD 80 0 WAVELENGTH 340nm TEMPERATURE 37C MEAS VOLUME 900ul WASH VOLUME 1000ul DELAY 3s DELTAS 5 TIME DELTA 5s MIN R 2 0 998 UNIT mg dl MEASURE BLANK Rb A 0 000 ST KIN 1 0 327 R 2 0 9996 ST KIN 2 0 330 R 2 0 9989 ST KIN 3 0 324 R 2 0 9994 ST KIN 0 327 FACTOR 244 3 NO ABS RESULT 1 0 232 41 5 R 2 0 9984 2 0 175 81 8 R 2 0 9997 Start method selection in the main menu See chapter 4 1 Mea...

Page 36: ...SH VOLUME 1000ul DELAY 2s UNIT MEASURE 100 NO ABS RESULT 1 0 329 46 9 2 1 004 9 9 3 2 020 1 0 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the print out of the method data follows The measuring window is shown Method procedure ÆInsert measure zero solution ÆInsert measure sample ÆInse...

Page 37: ... the blank is measured the time controlled measuring process will be started with RESULT With a combination of acoustic signals and text messages on the display the Photometer takes control of the timing for the whole measuring The measurement of the samples E1 can be finished at any time with E1 E2 In the second course will be so many samples measured as in the first course Before starting a new ...

Page 38: ...671 2 0 578 3 0 619 NO Sb A S A E1 1 0 000 1 012 2 0 000 1 138 3 0 000 1 076 NO Sb A S A E2 1 0 000 1 458 2 0 000 1 530 3 0 000 1 384 NO RESULT 1 0 446 2 0 392 3 0 307 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the print out of the method data follows The measuring window is shown M...

Page 39: ...nm Factor given entering Conversion factor µmol L 0 6206 mg dl PHOTOMETER 5010 7000 V5 13a 02 09 09 D LAB RIELE GMBH CO KG DATE 09 08 09 TIME 08 16 31 METHOD 15 C F 3 WL PROGRAM 15 FACTOR 1 00 WAVELENGTH 380 415 450nm TEMPERATURE 37C DELAY 2s UNIT mg dl MEASURE BLANK NO µmol L mg dl 1 4 859 7 83 2 5 865 9 45 Start method selection in the main menu See chapter 4 1 Measurement with programmed method...

Page 40: ... THE METHODS In Fig 6 4 all setting parameters can be edited METHODE DELETE Change to Fig 6 3 where the number of the method to be deleted is queried After a prompt for confirmation the selected method is deleted Basic and fixed methods cannot be deleted LIST A list of all programmed methods can be printed and transmitted via the serial inter face EXIT Return to main menu Fig 6 2 All methods from ...

Page 41: ...eter window 3 At least one control serum must be defined before data can be entered see chapter 7 2 6 Quality control If at least one control serum with its setpoint and range is entered corresponding memory of the quality control is reserved for this method So it can be supervised with inte grated quality control If both ID identifications are deleted then also all data and reserved memory of thi...

Page 42: ...r the curve bases P and P Consecutive numbering of the current bases A C Switch input between A for absorbance and C for concentration E Accept the edited value Input and confirmation of a single 0 at A lead to the deletion of the current pair of points In order to set the value to zero enter e g 0 0 ESC End input and save curve data For measuring in a multi standard method at least 2 bases with A...

Page 43: ... E N E W C H A N G E C O P Y U T I L I T I E S L A M P L F 3 7 0 5 C 0 9 0 8 0 9 1 1 3 9 3 7 0 5 C 0 9 0 8 0 9 1 1 3 9 U T I L I T I E S P A G E 1 5 D A R K A D J U S T M E N T P A G E M U L T I S T A N D A R D P R I N T E R P U M P M E N U E X I T Utilities Description in chapter Dark adjustment 7 2 1 Multi standard functions 7 2 2 Printer ON OFF 7 2 3 Pump menu 7 2 4 Serial interface 7 2 5 Quali...

Page 44: ...RT Automatically all eight sensitivity levels V0 V7 are calibrated in succession at simultaneous display of the dark value The function cannot be interrupted After com pletion the program returns to the utility program level Monthly executed the dark adjustment compensates possible deviations of the measuring accuracy due to environmental influences D A R K A D J U S T M E N T E X I T S T A R T D ...

Page 45: ...s are queried during the following procedure The program for measuring the multi standards branches out automatically to the calculation procedures as follows CP1 CP5 CP2 CP6 CP3 CP7 CP4 CP8 CP9 CP10 CP11 CP12 CP14 CP14 Print out of curve For measuring in another standard method than suggested start measuring multi standards in each other selected standard method before measuring the first standar...

Page 46: ... Accept the edited value Input and confirmation of a single 0 at A lead to the deletion of the current pair of points In order to set the value to zero enter e g 0 0 ESC End input and save curve data The bases are sorted in ascending order according to their A value C U R V E N O 2 0 E X I T C U R V E N O x x P 1 A 0 0 0 0 0 N E W A C 0 0 0 0 0 1 2 3 4 5 6 7 8 E S C 9 0 P P A C E 7 2 3 Printer ON ...

Page 47: ... Pump calibration The pump calibration can be executed with or without bubble detector So set bubble detec tor ON or OFF Empty aspiration tube by WASH Put 1000 µl distilled water into a sample cup Dip aspiration tube into the sample cup up to the base The volume of 1000 µl is sucked in by Sipping lever P Behavior with bubble detector After stoppage of the pump values for air and water are indicate...

Page 48: ...tically 7 2 4 3 1 Bubble detector ON OFF The current status of the bubble detector is indicated in the first line by OFF or ON Change setting by SELECT Save setting permanently by OK Save setting temporarily up to next switch off of the device by EXIT 7 2 4 3 2 Volume optimized ON OFF The current status of this setting is indicated in the first line by OFF or ON Change setting by SELECT Save setti...

Page 49: ...t the aspiration tube is completely filled with water Enter new sensitivity level step by step by numeric key 0 up to 7 starting with 0 until the display changes from AIR to WATER Then increase the sensitivity level by one further step Empty the aspiration tube by Sipping lever P The display changes from WATER to AIR The adjustment is permanently saved by E M E D I A T E S T 4 W A T E R 2 4 5 1 2 ...

Page 50: ...ange setting by SELECT EDP OFF no output EDP ON CR LF system output through serial port with CR LF protocol EDP ON STX ETX BCC system output through serial port with STX ETX BCC protocol EDP ON CR LF LOG after each measurement a formatted string will be output through the serial port e g see table 7 2 5 1 Save setting permanently by OK Save setting temporarily up to next switch off of the device b...

Page 51: ... stored with method number date and user identification From the daily memory the individual QC data can become deleted or saved in the monthly memory of the corresponding method The monthly memory of a QC method can record up to 31 readings With the 32nd the oldest reading is deleted in the memory For the calculation of the quality values of a method at least 20 readings in the monthly memory mus...

Page 52: ...E M O R Y E X I T S 1 S 2 UTILITY PROGRAMS DAILY MEMORY S1 PHOTOMETER 5010 7000 V5 13a 02 09 09 D LAB RIELE GMBH CO KG DATE 09 08 09 TIME 08 30 03 25 GLUCOSE 13 03 21 HDL C 367 27 UREA COL 197 2 29 CK MB 1128 1 31 GOT 189 9 Measuring data of the corresponding daily memory are shown with method number method name reading and dimension Change to next reading Change to previous reading DEL Delete sho...

Page 53: ...splayed For the calculation of the quality values of a method at least 20 readings in the month memory must be present 1 User identification The keys have following functions Change to next reading Change to previous reading DEL Delete all measuring data of the monthly memory of the selected method and confirm again e g at change of serum MORE Change to output dialog EXIT Return to QC menu C O N T...

Page 54: ...s 2 02 02 06 13 11 1s 1 02 01 06 13 88 1s 3 01 31 06 13 51 1s 3 01 30 06 13 24 1s 3 01 29 06 12 50 2s 1 01 28 06 12 74 2s 2 01 27 06 13 30 1s 1 Output dialog If at least 20 readings are stored in the monthly memory these are indicated in the Levey Jennings plot In this representation the deviations can be controlled visually and thus tendencies or systematic errors be better recognized The keys fo...

Page 55: ...RATURE E 25C 8322 3000 30C 9552 3000 37C 11273 3000 TEMPERATURE D 25C 8299 3000 30C 9485 3000 37C 11165 3000 FILTER 0 DDD 1 340 2 405 3 492 4 546 5 578 6 623 7 999 8 999 9 999 PUMP MS AIR 201 MS 50ul 66 B DET 5 VOLUME OPTIMIZED OFF BATTERY OK VDC LAMP 12 12V ADC CORRECTION 150 STACK TEST 14 BOOST nm 390 EDP OFF STX ETX BCC LANUAGE 1 ENGLISH 2 GERMAN KEY SIGNAL ON PROGR METHODS 0 By START the progr...

Page 56: ... first by deletion of results Print out of readings of an ID no DATA FROM ID NO PHOTOMETER 5010 7000 DATE 09 08 09 LAB RIELE GMBH CO KG ID NO 35 DATE 09 08 09 METHOD 24 GLUCOSE 13 03 mmol l DATE 09 08 09 METHOD 25 CK MB 195 U l DATE 09 08 09 METHOD 24 GLUCOSE 12 17 mmol l Report of logging memory LOGGING REPORT PHOTOMETER 5010 7000 DATE 09 08 09 5 3 11 2 14 2 35 3 The logging menu offers following...

Page 57: ...ed at the factory Anyhow the tempering control can be cali brated provided that the tempering was switched on for at least 30 minutes Measure the current temperature with an independent measuring system e g thermis tor REF 090 063 inside the cuvette and enter this value According to the difference to 25 0 C 30 0 C or 37 0 C the system corrects its internal setting The calibrating of the tem peratu...

Page 58: ...W E R T Y U I O P A S D F G H J K L Å E S C Z X C V B N M A 1 E N T 7 2 12 User name The names of maximum five users can be stored permanently After calling a method the user is queried In case of a stored name an additional line within the header is sent to the printer or to the EDP Select user by SELECT By NEW the entry of the user name is possible Enter the user name via the alphanumeric keyboa...

Page 59: ...ne for error messages remains active in any case K E Y S I G N A L O N E X I T O K S E L E C T 7 2 15 Touchscreen adjustment By this function the panel can be adjusted After call of the function a white cross is shown in the left lower corner of the screen Touch the intersection point in the cross with a non scratching plastic tip touchscreen pen pipette tip as exactly as possible In the first lin...

Page 60: ...E L E C T P H O T O M E T E R 4 0 4 0 5 0 1 0 7 2 16 Date Time The current status of the date time display is indicated in the first line by OFF or ON Change setting by SELECT With activation of the clock date and time can be changed by OK Each entry of day month year minute and second must be confirmed by E If a value is to be changed then all values are to be entered again D A T E T I M E O N E ...

Page 61: ...dark adjustment o U T I L I T I E S P A G E 5 5 A D C C O U N T S O P T I C B O O S T O F F V 0 F 1 3 4 0 n m 2 1 3 3 6 V 0 V 1 V 2 V 3 V 4 V 5 V 6 V 7 B O F 0 F 1 F 2 F 3 F 4 F 5 F 6 F 7 F 8 F 9 E S C Stop function by ESC 7 2 19 Send results Start sending stored results through the serial port by pressing START or cancel with EXIT After sending is completed you will be prompted to delete stored r...

Page 62: ...dist water 3 5 x wash with HCl 0 5 N 4 5 x rinse with dist water If the system is contaminated severely it may alternatively be cleaned with hypochlorite The following proce dure is advised step by step 1 5 x rinse with dist water 2 2 3 x wash with hypochlorite 1 20 diluted solution or ISE Cleaning Solution undiluted let it possibly affect up to 20 minutes 3 5 10 x rinse with dist water For device...

Page 63: ...ometer 5010 operates at any line voltage between 90 VAC and 264 VAC at 50 60 Hz without adjustment It has two line fuses in series with the power supply They are located on the rear panel To replace those fuses unplug the mains cable and remove the fuse holder with the fuses as shown below The instrument is delivered with two spare fuses Neither use makeshift fuses nor short circuit the fuse holde...

Page 64: ...the correct keyboard entry 9 3 PLAINTEXT ERROR MESSAGES RANGE MIN The programmed low limit was under run by the measurement RANGE MAX The programmed upper limit was exceeded by the measurement RANGE R 2 The square of the correlation coefficient R lies at the kinetic measuring below the programmed low limit RANGE At the kinetic measuring the procedure of the kinetic is wrong increasing falling NO M...

Page 65: ...ring solution 23 overflow at Kinetic check measuring solution 24 overflow at Kinetic check measuring solution 25 overflow at Kinetic check measuring solution 26 overflow at Kinetic check measuring solution 27 overflow at Kinetic check measuring solution 28 overflow at Kinetic check measuring solution 29 wrong input of deltas or time per delta restart method 30 battery empty contact service partner...

Page 66: ...ata points is missing Check multi standard functions 54 nonlinear measurement value out of range check the absorbance of the sample 55 number of given data points 2 add data points 57 memory of data logging management is completely filled delete log data chapter 7 2 8 58 checksum of data logging damaged delete log data chapter 7 2 8 59 error in the automatic measuring operation remote check interf...

Page 67: ...stible gases Free from vibrations Free from electromagnetic wave interference Well distanced from a machine generating a high frequency high voltage e g a centrifuge 10 2 MINIMAL OPERATION QUALITY Signal processing in analogue amplifiers with high amplification factors cannot differentiate desired from undesired signals Amplifiers thus are apt to be overloaded or produce spurious signals The equip...

Page 68: ... printer dot matrix 24 characters per line Languages English and German Indonesian Russian Spanish Memory General operating software can be updated by PC Reagent open system with capacity for up to 231 programmable methods Data import by touchscreen or PC Up to 50 nonlinear calibration curves with max 20 sets of points can be stored Signal Port Serial port for connection to an external printer and...

Page 69: ...the product Product name Photometer 5010 to which this declaration relates is in conformity with the following standards or other normative documents EN 61326 1 EN 61010 1 EN 61010 2 101 following the provisions of Directive 89 336 EWG EMC and 73 23 EWG LVD as well as 98 79 EG IVD This declaration describes an absorption photometer for laboratory use Berlin March 2006 ROBERT RIELE GmbH Co KG W Rie...

Page 70: ...en lamp B 1 3 Spectroscopic apparatus filter wheel B 1 4 Radiation detector s photodiode B 1 5 Cuvette s cell s 10 mm glass or plastic cuvette square shaped or 10 mm flow through cuvette B 1 6 Temperature regulation of the cuvette 25 C 30 C or 37 C B 1 7 Displayed units absorbance mass concentration enzyme activity B 1 8 Display device digital display absorbance 0 000 to 2 500 mass concentration 0...

Page 71: ...er limiting values B 5 1 Photometric uncertainty of the spectral absorbance max 3 B 5 2 Photometric short time variation coefficient 1 B 5 3 Uncertainty of wavelengths max 2 nm B 5 4 Spectral half width of spectral radiation flux at detector 10 nm B 5 5 Percentage of wavelength integrated false radiation measured at 340 nm as transmittance of a cut off filter NaNO3 0 1 B 5 6 Uncertainty of tempera...

Page 72: ...gen lamp 12V 20W 5010 024 Biogent A 1000 ml 0369624001 Interference filter 436nm 5010 670 Interference filter 670nm other wavelengths on request 500 002 Incubator T12 500 001 Incubator T16 1707175001 Joint inlet tube cuvette 0552402001 Mains cable 5010 005 Operator s manual 5010 066 Outlet tube cuvette 5 pcs 1268457001 Printer paper 5 rolls 1010735001 Printer ribbon 1 pc 0745235001 Printer ribbon ...

Page 73: ...s chapter 12 1 BASIC METHOD 16 19 free reserved for further 4 automatic calculation methods 20 250 up to 231 user specific methods chapter 12 2 LIST OF USER SPECIFIC METHODS as copy master to be filled out by the user 12 1 BASIC METHODS ROBERT RIELE GmbH Co KG Page 73 12 ...

Page 74: ...5010e_513 doc 22 01 10 12 2 LIST OF USER SPECIFIC METHODS ROBERT RIELE GmbH Co KG Page 74 12 METHOD LIST ...

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