5010e_513.doc / 22.01.10
3.5
CUVETTES AND CUVETTE ADAPTOR
3.5.1
Changing cuvette adaptor
If a flow system is installed, first the tubing system must be emptied: Go to the main menu and execute a
washing process by sipping lever [P] five times. Air is aspirated and the residual liquid is pumped off through the
waste tube.
Switch off the device.
If a flow system is installed, disconnect the bubble detector, remove the aspiration tube from the metal tube and
disconnect the outlet tube cuvette from the joints of the pump tube.
Loosen the milled screw and remove the adaptor.
Another adaptor can easily be inserted. Before check the cleanliness of the lense and the underside of the
adaptor. When inserting the adaptor electrical contacts in the device are closed. Turn milled screw only by hand!
3.5.2
Working with original flow-through system
Lead the aspiration tube with attached bubble detector without sharp bend from the working area through the
metal tube. Fasten outlet tube cuvette to the metal nipple of the cuvette as well as to the joint of the pump tube.
Before connecting the tubes remove pump tube at both ends from the metal clamps. The pump tube
must not be tensed during longer time out (fig. below).
Plug the bubble detector to the socket in the working area. It must sit as close as possible at the connection of
the flow-through cuvette. For working without bubble detector switch off this function (chapter 7.2.4.3 -
While working with the flow-through system the tubes must not be sharply bended. No residues should
be inside. Check from time to time that the tubing and the connections are leakproof. After each replacement of
tubing execute a
Before as well as after all measuring it is absolutely necessary to wash the tube system repeatedly with
distilled water or another appropriate rinse solution by pressing [WASH] or sipping lever [P]. This is also neces-
sary after a method change (chapter 8.1 - CLEANING INSTRUCTION). For the procedure within a measuring
series see the application regulation.
In order to aspirate solution into the measuring system put the aspiration tube deeply enough into the respective
vessel.
Before setting to zero press [ZERO]. Trigger setting to zero by sipping lever [P].
Trigger a normal measuring by sipping lever [P]. Repeat a measuring of a solution which is already aspirated by
[RESULT].
To work in optimized volume mode use the function
function makes it possible, for example, to pump two consecutive times 500 µl from 1000 µl sample volume.
Tension release:
The upper connection of
the pump tube can be
loosened easily from the
metal clamps.
ROBERT RIELE GmbH & Co KG
Page 12
3 - OPERATING ELEMENTS
