Promega Maxwell 16 AS1000 Operating Manual Download Page 1

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T e c h n i c a l M a n u a l

Maxwell

16 Instrument

Operating Manual

INSTRUCTIONS FOR USE OF PRODUCTS AS1000 AND AS1002.

PRINTED IN USA. Revised 6/08

Part# TM274

Note:

Please ensure that all sealing tape and any residual adhesive are removed from

the Maxwell

16 system cartridges before placing the cartridges into the instrument.

tm274.0608.qxp 6/4/2008 9:40 AM Page a

Summary of Contents for Maxwell 16 AS1000

Page 1: ...FOR USE OF PRODUCTS AS1000 AND AS1002 PRINTED IN USA Revised 6 08 Part TM274 Note Please ensure that all sealing tape and any residual adhesive are removed from the Maxwell 16 system cartridges before...

Page 2: ...tional Mode 18 ii Changing the Maxwell 16 Instrument Operational Mode 18 IV Operating the Maxwell 16 Instrument 20 A Navigation 20 B Operational Qualification 20 C Sample Purification 21 V Periodic Cl...

Page 3: ...er preference The Maxwell 16 System purifies samples using paramagnetic particles PMPs which provide a mobile solid phase that optimizes capture washing and elution of the target material The Maxwell...

Page 4: ...e Upgrades 1 1 5mm Hex Wrench 1 Maxwell 16 LEV Cartridge Rack 1 Operating Manual and Warranty Information 1 Quick Start Guide Available Separately Product Cat Maxwell 16 SEV Hardware Kit AS1200 Requir...

Page 5: ...ack 5296MB A B C D E F I C Inspection Upon receiving your Maxwell 16 Instrument please inspect the package carefully to make sure all accessories are present Standard accessories are shown in Figure 1...

Page 6: ...A Part TM274 Revised 6 08 Page 5 I C Inspection continued Figure 2 Maxwell 16 LEV Instrument AS1002 accessories Low elution volume configuration A Operating Manual Warranty Information and Quick Start...

Page 7: ...800 356 9526 Phone 608 274 4330 Fax 608 277 2516 www promega com Part TM274 Printed in USA Page 6 Revised 6 08 Safety Symbols and Markings Symboles de S curit et Marquages Danger Hazardous voltage Ris...

Page 8: ...e Use only Promega Maxwell 16 cartridges and plungers designed for use with the instrument Utiliser uniquement des cartouches et pistons Promega Maxwell 16 con us pour une utilisation avec l instrumen...

Page 9: ...nstall in a location where there is large temperature variability or high humidity Do not position the instrument so that it is difficult to unplug from the power source Do not place next to heat sour...

Page 10: ...display panel A LCD display B Keypad including Run Stop Menu Scroll Up and Scroll Down buttons Maxwell 16 components A Standard configuration B LEV configuration C Maxwell 16 platform A B Linear slid...

Page 11: ...plug in or turn on the machine before removing the shipping anchors Turning on the Maxwell 16 Instrument before the shipping anchors are removed will cause a lot of noise but will not result in perma...

Page 12: ...Once the anchor is loose slide the shipping anchor B up to the top of the linear slide to be even with anchors A and D If the anchor comes off the slide place it back on at the top of the linear slide...

Page 13: ...lides A D B C 11 Locate the bottom magnetic rod assembly shipping anchors There are a total of 4 anchors E H To locate these anchors gently push the magnetic rod assembly and plunger bar up so that yo...

Page 14: ...sembly Plunger Bar shipping anchors have been moved from their shipping anchor positions and all inside packaging materials have been removed you can change the instrument Magnetic Rod Assembly Plunge...

Page 15: ...ld then be fairly difficult to remove 6 Insert the black LEV Magnetic Rod Assembly The set screws should be facing toward you Press firmly to place the LEV Magnetic Rod Assembly in position It will fi...

Page 16: ...tronics 3 Unscrew the three thumbscrews on top of the magnetic rod assembly 4 Gently lift up the LEV Magnetic Rod Assembly to remove it 6029TB 5 Pull down on the LEV Plunger Bar Adaptor to release the...

Page 17: ...wer cord into the back of the Maxwell 16 Instrument 4 Plug the power cord into a wall outlet See Section I A for power requirements 5 Close the door 6 Turn the machine on 7 Once turned on the instrume...

Page 18: ...Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 Phone 608 274 4330 Fax 608 277 2516 www promega com Printed in USA Part TM274 Revised 6 08 Page 17 Maxwell 16 Kit Purificatio...

Page 19: ...the Standard Elution Volume hardware select SEV If the instrument is configured with the Low Elution Volume hardware select LEV Press Run Stop to select 6035MB 1 SEV Hardware 2 LEV Hardware Hardware...

Page 20: ...ger Bar Press Run Stop Key Hardware Setup Add SEV hardware Magnet Assembly Press Run Stop Key If changing from SEV to LEV the following screens will appear 6220MA Hardware Setup Remove SEV hardware Ma...

Page 21: ...k Successful or Calibration Error During instrument runs During instrument runs the instrument will automatically do periodic self diagnostic tests to verify that the platform plunger bar and magnetic...

Page 22: ...y hazardous chemicals Users should wear protective gloves or other protective means when handling the reagent cartridges Users should follow their institutional guidelines for disposal Note Following...

Page 23: ...ampened with deionized water or 70 ethanol Keep the cooling vents in the back of the machine clear of dust Do not remove the Maxwell 16 Instrument case for cleaning This will void the warranty Do not...

Page 24: ...s 3 Unscrew and remove the three thumbscrews on top of the magnetic rod assembly Figure 5 4 Once the three thumbscrews are removed gently lift up the magnetic rod assembly to remove it 5 To clean the...

Page 25: ...ntact Promega or your local Promega representative for service Heater error at the elution step Something is wrong with the electrical heating system Contact Promega or your local Promega representati...

Page 26: ...urn the instrument off wait a few seconds then turn the instrument back on If the calibration error persists please contact Promega or your local Promega representative for service Calibration error E...

Page 27: ...strument off wait a few seconds then turn the instrument back on If the calibration error persists please contact Promega or your local Promega representative for service Power failure occurs during i...

Page 28: ...a run by turning the unit off and then back on again This only needs to be done once after upgrading firmware The screen should now display the new firmware version Disconnect the RS 232 Cable from t...

Page 29: ...nt as far as it will go 6 By hand gently lower the plunger bar and magnetic rod assembly down as far as it will go The bottom of the plunger bar and the magnetic rods should be pressed slightly into t...

Page 30: ...agnetic rod assembly down as far as it will go The bottom of the magnetic rods should be pressed into the foam packaging material that is sitting on top of the platform 10 Locate the shipping anchor B...

Page 31: ...Power Cable into the box 6 Affix the Certificate of Decontamination on the outside of the shipping box Write the return authorization number provided to you by Promega or your local Promega represent...

Page 32: ...h from the instrument surfaces Repeat the procedure as many times as required to effectively disinfect and decontaminate the instrument Failure to confirm disinfection and decontamination will result...

Page 33: ...following steps 1 Write or call the company that sold you the instrument and describe as precisely as possible the nature of the problem 2 Carry out minor adjustments or tests as suggested by your te...

Page 34: ...s AS1050 For Laboratory Use Sufficient for 48 automated isolations from tissue cells or blood sample types Product Size Cat Maxwell 16 Tissue LEV Total RNA Purification Kit 48 preps AS1220 For Laborat...

Page 35: ...nship according to the terms of the limited warranty with the addition of If during the warranty period the instrument is discovered to be defective Promega will provide the temporary use of a replace...

Page 36: ...2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 Phone 608 274 4330 Fax 608 277 2516 www promega com Printed in USA Part TM274 Revised 6 08 Page 35 tm274 0608 qxp 6 4 20...

Page 37: ...DNA IQ is a trademark of Promega Corporation Windows is a registered trademark of Microsoft Corporation Products may be covered by pending or issued patents or may have certain limitations Please vis...

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