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IntelliPlex

1000



Code User Manual - Jan-20

Notice to User

The use of this instrumentation and its associated software is covered by one or more pending

US and foreign patents owned by PlexBio Co., Ltd. The purchase of this instrument and software

includes nontransferable rights to use the instrument and software to practice the methods

described therein. No general patent or other license of any kind other than this specific right of

use from purchase is granted. For further information on purchasing licenses for other

applications can be obtained from PlexBio Co., Ltd. 6F-1, No. 351, Yangguang St., Neihu District,

Taipei City 11491, Taiwan.

Trademarks

,DeXipher

, IntelliPlex

, DigiPlex

, πCode

, PlexBio

, are trademarks

or registered trademarks of PlexBio Co., Ltd. All other product names and any registered and

unregistered trademarks mentioned are used for identification purposes only and remain the

exclusive property of their respective owners.

Copyright

Summary of Contents for IntelliPlex IW100

Page 1: ...er Manual PlexBio Co Ltd PlexBio Co Ltd 6F 1 No 351 Yangguang St Neihu Dist Taipei City 11491 Taiwan Tel 886 2 2627 5878 Fax 886 2 2627 5979 http www plexbio com A04 016 V05 RUO For Research Use Only...

Page 2: ...haracteristics 7 3 2 1 Shaking and Incubation 7 3 2 2 Washing 7 3 2 3 Fluorescence Labeling 7 3 3 System Technical Specification 8 Chapter 4 Receiving the Instrument 9 4 1 Inspect the Instrument Packa...

Page 3: ...Log Status 41 6 11 Shutdown 44 Chapter 7 Maintenance 45 7 1 Recommended Maintenance Schedule 45 7 2 As Needed Maintenance 46 7 2 1 Clean Fluid Path Tubes with 70 Ethanol 46 7 2 2 Clean and Disinfected...

Page 4: ...eling Technical Information Installation Operation Procedures Settings Maintenance 1 2 Warnings When operated in a safe environment according to the instructions in this user manual there are no known...

Page 5: ...ons instructions and regulatory agencies General cautions and warnings Caution pinch point Warning Biohazard Warning Hot Surface Manufacturer Date of Manufacture Consult instructions for use Batch cod...

Page 6: ...rkstation and is intended to be used with dedicated 96 well plates for sample washing shaking incubation and automated fluorescence labeling The workstation is specifically designed for the CodeTM Mic...

Page 7: ...excessive force on the stage or it may become tilted or bent This will severely interfere with the performance of the system This message is provided to avoid electric shock or possible damage to the...

Page 8: ...tious materials Please use personal protective equipment PPE including gloves and laboratory coats Please handle with extreme care when loading the plate onto the stage to prevent spilling Refer to Ch...

Page 9: ...cede fluorescent detection by PlexBio 100 Fluorescent Analyzer The IntelliPlex 1000 Code Processor minimizes user s hands on time and supports set up of up to six custom protocols from hybridization t...

Page 10: ...for compatible 96 well microplates Ready to run built in PlexBio assay programs 3 2 2 Washing This function is designed to reduce hands on time and human error during the Code MicroDiscs washing proce...

Page 11: ...itions 8 way manifold 150 L PlexBio Wash Buffer MicroDisc loss Around 75 of MicroDiscs retained after whole processes Measured under following condition 500 1500 discs well in 150 L PlexBio Wash Buffe...

Page 12: ...on area of 0 36 square meters and needs to be placed at least 10 centimeters from the wall for components connection and efficient cooling 4 1 Inspect the Instrument Package IntelliPlex 1000 Code Proc...

Page 13: ...ll be inspected during installation process Do not open the box before installation Fig 3 1 Accessories in Top EPE foam packaging Fig 3 2 Accessories in the box Tubes 3 Manual 1 Accessory box 1 Waste...

Page 14: ...e warranty Moving the IntelliPlex 1000 Code Processor is not allowed If moving is required please contact Customer Service of PlexBio Co Ltd or qualified local distributors Before operation connect th...

Page 15: ...The system will run temperature check and system self test every time the instrument is powered on Make sure the ambient temperature is appropriate for instrument operation the buffer water bottles an...

Page 16: ...ction buttons for different assay types Built in PlexBio Molecular Assay Built in PlexBio Immunoassay Built in PlexBio Applied Science Custom Program 2 Menu button to access settings and maintenance p...

Page 17: ...in PlexBio assay type Molecular Assay Immunoassay or Applied Science Step 2 Fig 9 Select the desired lanes for processing and click Next Step 3 Fig 10 Select the corresponding assay to operate Step 4...

Page 18: ...15 IntelliPlexTM 1000 Code User Manual Jan 20 Fig 9 Select the desired lanes for processing Fig 10 Select the corresponding assay One Lane...

Page 19: ...16 IntelliPlexTM 1000 Code User Manual Jan 20 Fig 11 Procedures confirmation Fig 12 Pop up window confirmation...

Page 20: ...17 IntelliPlexTM 1000 Code User Manual Jan 20 Fig 13 Real time display of the assay operation status...

Page 21: ...om Program The IntelliPlex 1000 Code Processor also allows users to create and modify custom assay programs The following section describes how to setup or edit a custom assay program a total of six c...

Page 22: ...t until the heating plate has cooled down A pop up will inform the user of any problems incubating at the selected incubation temperature Please be aware that custom programs with multiple heating ste...

Page 23: ...lexTM 1000 Code User Manual Jan 20 temperature If not activated shaking and incubation time countdown will start once the incubation temperature has been reached Fig 15 Parameters of custom incubation...

Page 24: ...function is not activated no liquid will be left in wells after washing process It is recommended to only activate this function if the wash step ends the program and the plate is subsequently analyze...

Page 25: ...rescent Analyzer The incubation period can be adjusted from 1 sec to 23hr 59min 59sec Please ensure that enough SA PE solution is added into V Tray before the program starts See Section 5 5 Special Ap...

Page 26: ...ditional reagent manually or refill change the solution in the V Tray we recommend using a new plastic V Tray for each solution Once the pause function is reached in an active program the run will be...

Page 27: ...2 Set detailed parameters for each procedure like temperature time washing cycles etc and save the procedure optional Step 5 Repeat Step 3 and 4 to add all desired procedures to the custom program Ste...

Page 28: ...25 IntelliPlexTM 1000 Code User Manual Jan 20 Fig 19 Custom Program Home Screen and Well Selection Fig 20 Custom Program 1 to 6...

Page 29: ...26 IntelliPlexTM 1000 Code User Manual Jan 20 Fig 21 Select desired procedures to set up a process Fig 22 Set detailed parameters for each process...

Page 30: ...27 IntelliPlexTM 1000 Code User Manual Jan 20 Fig 23 Custom Program Process and Real time Display...

Page 31: ...wells A to H see schematic representation below in an assay is 400 L Please note that the dead volume of the V Tray is 500 L for up to 6 selected lanes or 800 L for more than 6 selected lanes 7 12 lan...

Page 32: ...ocessor The prepared 1X Wash Buffer can be used for up to 1 week Do not use the buffer if visible contamination e g fungus occurs Please discard of contaminated Wash Buffer according to local requirem...

Page 33: ...ests 3 wash cycles and 1 SAPE reaction 150 mL DNA RNA program for 3 lanes up to 24 tests 3 wash cycles and 1 SAPE reaction 220 mL DNA RNA program for 6 lanes up to 48 tests 3 wash cycles and 1 SAPE re...

Page 34: ...1000 Code User Manual Jan 20 Chapter 6 System Settings To get to the System Menu click on top left screen corner The Settings menu is shown in Fig 25 Each section is described in detail below Fig 25 T...

Page 35: ...32 IntelliPlexTM 1000 Code User Manual Jan 20 6 1 Maintenance This section describes how to renew the solution U Trays and clean the manifold Fig 26 Fig 26 Maintenance from tool menu...

Page 36: ...tenance function in the settings menu Fig 27 Positions of the Washing and SA PE Tanks To renew the U Tray please follow the steps below Step 1 Open the System Menu Step 2 Select Setting on the menu pa...

Page 37: ...ut the transparent U Tray placed in the white tank module Operating the IntelliPlex 1000 Code Processor without the plastic U Tray placed in the white module may damage the IntelliPlex 1000 Code Proce...

Page 38: ...m SA PE tank Step 2 Open the System Menu Step 3 Select Setting on the menu page Step 4 Click on the Maintenance button Step 5 Select Clean Manifold and then click OK in the pop up window The dispensin...

Page 39: ...is function returns the manifold the thermal lid and the magnetic plate back to their original positions Step 1 Select Setting in the menu Step 2 Choose Instrument Reset to reset all modules Step 3 Cl...

Page 40: ...he PRIME function which will prime the tubing with Wash Buffer The PRIME function can be performed immediately after the RINSE function or later but must be completed before performing the next run Pl...

Page 41: ...used during monthly maintenance and long term shutdown procedure see Chapter 7 for details Step 1 Open the System Menu Step 2 Select Setting on the menu page Step 3 Select Fluid Path on the tool menu...

Page 42: ...act PlexBio Co Ltd or local distributors for more information 6 6 Assay Update This function allows to update the built in assay protocols PlexBio Co Ltd or your local distributor will inform you once...

Page 43: ...TM 1000 Code User Manual Jan 20 6 7 Date Time Setup the current Date and Time for the instruments Fig 33 Date and time setting 6 8 Language Set the language to English or Simplified Chinese Fig 34 Lan...

Page 44: ...eer Do not access the service mode unless specific instructions are given by PlexBio technical support Fig 35 Service Mode 6 10 Log Status To view the system log history Step 1 Select the Log Status o...

Page 45: ...n USB drive with FAT32 format and at least 2GB capacity available Fig 36 USB drive requirements Step 2 Create a new folder named PlexbioUpdate Step 3 Insert the USB drive into the USB port below the t...

Page 46: ...Jan 20 Step 5 Click EXPORT on the bottom right to export log files Fig 38 Export log files Step 6 Pull out the USB drive after the process is completed Step 7 Connect the USB drive to a computer to ch...

Page 47: ...ensure that the pump and tubes are rinsed and all modules are at their proper position Never turn off the power on the backside of the instrument without running the Shutdown program first Step 1 Open...

Page 48: ...rformance It is required to follow the schedule below Operating the IntelliPlex 1000 Code Processor without proper maintenance will void its warranty Please read and follow the section 7 2 As Needed M...

Page 49: ...n paper towel Connect the wash buffer lid to the bottle with 70 ethanol Ensure the straw is submerged in ethanol Step 7 Run the Prime cycle of the Fluid Path on the setting menu four times refer to se...

Page 50: ...hed on off at least once a week The Long term Shutdown procedure cleans the fluid path with water to remove all Wash Buffer followed by a 70 ethanol rinse to reduce the possibility of salt crystal for...

Page 51: ...ep 1 Open the air valves of the ddH2O and Wash Buffer lids Step 2 Do not fill the ddH2O and Wash Buffer bottles Step 3 Ensure that the on off switch in the rear panel of the instrument is in the off p...

Page 52: ...fill up the ddH2O bottle with distilled water Step 5 Connect the buffer tubes Wash buffer and Distilled water to the inlet connectors on the rear panel of the instrument and to the corresponding bott...

Page 53: ...5 C The system will be locked Contact PLEXBIO or local distributors for service 8 2001 The shaker motor does not return to the defined home position Reboot the machine and perform self test Contact PL...

Page 54: ...ted to the end of incubation The system will be locked Contact PLEXBIO or local distributors for service 19 6501 The temperature appears to be lower than operation requirement 18 C during the temperat...

Page 55: ...Contact Information PlexBio Co Ltd 6F 1 No 351 Yangguang St Neihu Dist Taipei City 11491 Taiwan Customer Service and Sales Web http www plexbio com Phone 886 2 2627 5878 Fax 886 2 2627 5979 Order info...

Page 56: ...titute the contract which has binding effect on the buyer under law Where the buyer disagrees with the Standard Terms he she shall contact PlexBio immediately to return the products before using the p...

Page 57: ...ces and notifies PlexBio of the same in writing within 30 days after the related services are performed PlexBio shall be obligated to provide necessary services assistance and consultation to correct...

Page 58: ...e clause applies to Products provided by PlexBio within the territory of Taiwan only The maintenance includes correction verification and regular maintenance works only PlexBio does not provide any wa...

Page 59: ...y kind other than this specific right of use from purchase is granted For further information on purchasing licenses for other applications can be obtained from PlexBio Co Ltd 6F 1 No 351 Yangguang St...

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