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IntelliPlex
TM
1000
Code User Manual - Jan-20
5.4.1 Custom Assay Parameters
Incubation:
(A)
Heater ON: Setup the desired incubation temperature by either sliding the setting bar or by
keying in the number into the gray box. The offered temperature range is 20°C to 60°C. It is
important to highlight that the selected incubation temperature must be 5°C above the
current temperature*. Please note that the IntelliPlex 1000 πCode Processor does not have
active cooling system. If a new run follows a previous run, please wait until the heating plate
has cooled down.
*Current temperature refers to the temperature of the heating plate. Note that the IntelliPlex
1000 πCode
Processor measures environmental temperature using the temperature of the heating plate itself. The
temperature inside the instrument can be a little higher. Thus, when the incubation temperature is too close to
the environmental temperature, error may occur.
For example, when the current temperature is 30°C, the incubation temperature must be at
least 35°C or higher to successfully run the program. If the plate is still warm from the
previous incubation process, please wait until the heating plate has cooled down. A pop-up
will inform the user of any problems incubating at the selected incubation temperature.
Please be aware that custom programs with multiple heating steps in a row may cause
termination of the run if the previous temperature was significantly higher than the
following temperature.
For example, if a first incubation is carried out at 50°C and immediately followed by a second
incubation step at 30°C, an error occurs, and the program will be stopped. Including a wash
step between two incubation at different temperatures may be enough to allow passive cool
down of the heating plate.
(B)
Incubation period: Set up the desired incubation time by directly keying in the numbers into
the hour (h), minute (m), and second (s) boxes. The time setting ranges from 1 sec to 23hr
59min 59sec.
(C)
Close lid during incubation: This function will decide if the lid is closed during incubation.
The lid also includes a heating function. Closing lid during incubation helps in keeping
samples more evenly heated. Please note that the lid does not seal the 96-well plate, thus
does not prevent evaporation at elevated incubation temperatures.
(D)
Shake before reaching temp.: If activated, heating plate shaking and incubation time
countdown start once the program has started, independent of the status of the incubation
