21
IntelliPlex
TM
1000
Code User Manual - Jan-20
Washing:
Users can setup various conditions listed below during the washing process (Fig. 16).
(A)
Number of Washing Cycles: Key in the desired number of washing cycles.
(B)
Washing Volume: Users can either select the 150
L washing volume (used for all πCode
applications) or the ELISA wash function (which will dispense 200
L wash buffer).
(C)
Last Dispense Volume: If the function is activated, additional 150
L of wash buffer will be
dispensed into each well at the end of the wash cycles followed by 10 sec shaking to spread
out/disperse the πCode evenly. If the function is not activated, no liquid will be left in wells
after washing process. It is recommended to only activate this function if the wash step ends
the program and the plate is subsequently analyzed by the PlexBio 100 Fluorescence
Analyzer.
(D)
Magnetic: Must be activated for all πCode assays. The magnet will collect and retain πCode
at the bottom of the well and only the liquid waste will be aspirated during washing process.
The magnetic function is not needed for ELISA assays.
(E)
Clean Tip: To prevent any carryover contamination between samples placed in different
wells, click on this function and the tips will be cleaned before approaching to next set of
wells. The tip cleaning will only be performed at the 1st cycle of each washing process.
Fig. 16. Parameters of custom washing procedure
