PerkinElmer Raman Micro 200 Series, Getting Started Manual

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100 . RamanMicro 200 Series Getting Started Guide
11. Repeat steps 2–10 for each objective lens.
12. Remove the intensity correction standard and store safely.
Any data acquired will now be intensity corrected.
Remember that if you have a fiber optic probe, you must intensity calibrate the fiber optic
probe as well as the microscope objective lenses. Ensure that the fiber optic probe has a lens
cap in place during intensity calibration of the objectives.
Summary
•
The only calibration procedure that needs to be performed on a regular basis is the
wavelength calibration verification procedure.
• The wavelength and laser frequency calibrations should only be performed if the
instrument fails the wavelength calibration verification procedure.
• The intensity calibration should only be performed on a yearly basis or if the
environment (temperature and / or humidity) changes significantly, or if the instrument
is moved or repaired.