PerkinElmer Lambda 650, Hardware Manual

Page: 50 / 72

50 . Using the Instrument
5.
Make horizontal fine alignment to the sample cell holder (locking screws and milled
posts loosened) to obtain the highest possible transmittance reading on the display.
Close sample compartment cover while measuring transmittance.
6.
Make fine alignment using the vertical adjustment screw again to obtain the highest
possible reading.
Close sample compartment cover while measuring transmittance.
7.
When you are satisfied with the alignment, tighten the milled posts and the locking
screws on the cell holder.
8.
Reinstall the reference cell in the reference cell holder.
9.
The sample cell remains in its holder.
10.
Repeat steps 4 to 6 with the reference cell holder, but this time obtain the lowest
possible transmittance reading on the display.
This completes the fine alignment procedure.
NOTE:
When the cell holder has been aligned once, you can take it out and reinstall it without
aligning it again.
Minimum Volume Applications
To measure minimum sample volumes, use microcells (offered by PerkinElmer).
The minimum sample volume required is a function of the cell internal width or volume and
is specified below.