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 LabGard® ES Energy Saver Class II,  

Type B2 Laminar Flow 

Biosafety Fume Hood/Cabinet 

 

Models  

NU-565-400/600 

Bench/Console 

 

Operation & Maintenance Manual 

 

 

 

 

 

(Series C) 

Revision 1 

August 2020 

 

 

 

 

 

 

 

                                                                  

 

 

 

 

 

Manufactured By: 

Nuaire, Inc. 

2100 Fernbrook Lane 

Plymouth, MN  55447 

Toll-Free: 1-800-328-3352 

In Minnesota: (763)-553-1270 

Fax: (763)-553-0459 

 

 

 

 

 

Summary of Contents for LabGard ES NU-565-400

Page 1: ...Flow Biosafety Fume Hood Cabinet Models NU 565 400 600 Bench Console Operation Maintenance Manual Series C Revision 1 August 2020 Manufactured By Nuaire Inc 2100 Fernbrook Lane Plymouth MN 55447 Toll...

Page 2: ...m particulate contaminants as prescribed in NSF ANSI 49 Please read this manual carefully to familiarize you with proper installation maintenance and operation of the cabinet Other reference and guide...

Page 3: ...p drawings are appended in the back flap of this manual along with any modifications and or additions to procedures as outlined in this manual A copy of the original factory test report is also append...

Page 4: ...EPA Filter Leak Test 7 7 Airflow Smoke Pattern Test 7 8 Site Installation Assessment Tests 7 9 Cleanliness Classification Test for Pharmacy Application 7 10 Main Control Board Description Replacement...

Page 5: ...d with proper technique is an effective laboratory aid in obtaining the optimum control over product quality while reducing the potential for exposure of both product and personnel to airborne biologi...

Page 6: ...apply while specific agents are used o Which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authorized expert personnel Keep these op...

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Page 8: ...OM0301 August 20 Page 8 of 105 2 0 Models Features The model NU 565 LABGARD ES Class II Type B2 Laminar Flow Biosafety Fume Hood Cabinet is manufactured in two sizes 4 ft 1 2m and 6 ft 1 8m...

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Page 12: ...ipments 4 1 1 Terms are factory unless stated otherwise Therefore it is important to check each shipment before acceptance 4 1 2 If there is visible damage the material can be accepted after the drive...

Page 13: ...accessible If the outlet is inaccessible such as a conduit hardwired connection then an appropriate warning label should be applied near the cabinet s on off switch to indicate the circuit breaker on...

Page 14: ...ecure See BCD 16300 5 2 2 Leveling Using a level placed on the work tray adjust the leg levelers first end to end then front to back The NSF approved leg levelers provide a 3 4 20mm adjustment 5 2 3 B...

Page 15: ...emote controlled needle valve plumbing fixtures Nuaire provides for rear bottom or top connections of plumbing services to plant utilities Connection from the needle valve assembly to the welded exit...

Page 16: ...e A minimum of 20 gauge for round duct is required to prevent duct collapse due to high static pressure conditions square duct will require heavier gauge material 8 All ductwork should be maintained a...

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Page 19: ...ume and corresponding inflow velocity is displayed on the front panel Preset lower and upper alarm limits are factory set but can be field verified at any time To ensure that adequate exhaust is avail...

Page 20: ...tch operation utilizing independent exhaust volume measurement instrument DIM Per NSF ANSI 49 a 20 loss of exhaust volume must produce an airflow alarm within 15 seconds The testing methods and equipm...

Page 21: ...bled Incl Exh Filter Fastener Height Minimum for Transport Width Depth with Armrest removed 62 1575 62 1575 53 5 8 1362 32 3 8 823 62 1575 62 1575 77 5 8 1972 32 3 8 823 Work Access Opening Inches mm...

Page 22: ...ital dual thermistor airflow sensor in the downflow stream to monitor and control airflow to setpoints The control system automatically compensates for filter loading voltage variances and other envir...

Page 23: ...be accessed for additional user menus 6 1 3 Run Mode Anytime the blower icon is selected the Run Mode screen will appear The Run Mode screen will display set points and the countdown of a 2 minute war...

Page 24: ...w the icons is the message area that will indicate alarms errors or other notable conditions Since the message area is limited to one line of text only the highest priority message will be indicated H...

Page 25: ...sed will provide a list of menu items for various TouchLink functions 6 1 5 1 Setpoint Limits This menu screen will indicate the Biosafety Fume Hood Cabinet airflow control setpoints Access is restric...

Page 26: ...setting text will provide a list of time functions available for use below is a description of each timer function Timer Functions Purge Timer This timer controls how long the blower will run to purge...

Page 27: ...UV light If timer is zero UV light will stay on until manually turned OFF or window is opened breaking the safety interlock Auto Run Timers This timer provides the ability to program on a daily basis...

Page 28: ...age 28 of 105 Once into the selected day press on window to enter the on off times Use the keypad to enter hours or minutes Press menu icon SAVE after each time entry Repeat auto timer function for ea...

Page 29: ...5 3 Historical Performance When press a historical performance text the system displays downflow and inflow history Downflow History press downflow history the system displays the week log data in gra...

Page 30: ...Inflow History press downflow history the system displays the week log data in graphical form of Inflow versus days Exhaust History press Exhaust history the system displays the week log data in graph...

Page 31: ...r service personnel to set up and calibrate the cabinet for certification or commissioning 6 1 5 5 Display Setting This menu item provides the ability to alter LCD screen display background contract a...

Page 32: ...d Gray can be selected Touch display tone audible feedback can turn on off Display brightness can be adjusted Languages may be selected of English Spanish French and German Time Date This menu item pr...

Page 33: ...0301 August 20 Page 33 of 105 6 1 5 6 Change User Password This menu item provides the ability to change the user password from the default value of 1234 Set Password Enter Old Password Set New Passwo...

Page 34: ...OM0301 August 20 Page 34 of 105 Retype New Password 6 1 5 7 System Information This menu item provides the cabinet model number serial number and software revision...

Page 35: ...ding window can be closed and the UV light turned on if installed NOTE If night setback exhaust airflow is reduced by the Building Automation System BAS and not by the Nuaire Model NU 951 012 valve it...

Page 36: ...by appropriate safety personnel 6 2 2 Minimize Penetration of Air Curtain The minimum number of items necessary should be placed into the cabinet to prevent overloading but the work should also be pl...

Page 37: ...s precaution is merely an extension of good aseptic technique as practiced on open bench tops The good laboratory practices designed to minimize creation and or release of aerosols to the environment...

Page 38: ...minated materials over uninoculated cultures or clean glassware should be avoided and transfer of viable materials should be performed as deeply into the cabinet away from open face as possible 6 3 4...

Page 39: ...ing seat and back control for personalized adjustment to assure proper user posture Be sure feet are resting on the floor chair foot support or foot rest Also be sure back is fully supported with prop...

Page 40: ...position With the view screen in the full open position personnel protection is compromised and a full faced HEPA filtered respirator must be worn c Clean all readily accessible surfaces of the cabin...

Page 41: ...f the sample chamber in the safety cabinet that is to be disinfected The formaldehyde evaporates immediately after reaching its boiling point the minimum reaction time is 6 hours Therefore the formald...

Page 42: ...s Normally no preventive maintenance is required on the interior of the cabinet i e the area behind the access panel containing the HEPA filters and motor blower assembly All required adjustments in o...

Page 43: ...e exhaust system 7 3 1 Supply Filter Replacement see Drawing BCD 19032 Disconnect electrical power from the cabinet before attempting any maintenance action Step 1 Remove screws at each upper side of...

Page 44: ...PA seal frame clockwise until the gasket is visually depressed by 1 8 inch 3mm The procedure for replacing the exhaust filter with the Bag in Bag out option is shown on Drawing BCD 05660 7 3 3 Motor B...

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Page 46: ...at the airflow monitor sensors are calibrated to read the correct values Press MENU to access Calibration Service parameter Entry into the Calibration Service functions requires a service password for...

Page 47: ...n the control system and is factory set and shouldn t require alteration The cabinet type default information controls unit of measure setpoints and limits based on the type and size of cabinet The OE...

Page 48: ...flow area workzone exhaust duct diameter size or special work access opening window height these must be entered into the control system to assure the correct display values Press MENU to access these...

Page 49: ...or altered Typically these default values are factory set based on the cabinet type model and size as previously discussed However they may be altered in special cases for modified cabinets The setpoi...

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Page 51: ...r NU 565 600 Downflow setpoint 60 Downflow setpoint 60 Downflow high limit 66 Downflow high limit 66 Downflow low limit 54 Downflow low limit 54 Exhaust setpoint 740 Exhaust setpoint 1122 Exhaust high...

Page 52: ...e necessary for proper cabinet performance The cabinet is considered to be certifiable if the following airflow measurements are present a Downflow average 60 LFPM 5 LFPM 30 m s 025 m s b Inflow avera...

Page 53: ...ct inflow velocity Use Table 7 1 to relate downflow and exhaust inflow volumes and corresponding average airflow velocities It is always desirable to achieve airflow values that are set as close as po...

Page 54: ...stated below Model Low Alarm Limit Pressure Switch Low Trip Point CFM CMH CFM CMH NU 565 400 629 1069 529 899 NU 565 600 954 1621 854 1451 If present remove cardboard from supply air intake and turn...

Page 55: ...ption Set Up Step 1 Activate power to the cabinet Turn on exhaust system and make sure damper actuator is plugged into the 3 position connector on the top right side of the cabinet Access sensor setup...

Page 56: ...city using the recommended procedure found in Table 7 0 If necessary adjust the exhaust system and or damper to achieve the correct inflow velocity Use Table 7 1 to relate downflow and exhaust inflow...

Page 57: ...ft sides of the control box and rotate control box forward to access the pressure switch adjustment Pressure switch must be adjusted while control box is open Adjust as needed the adjustment dial unti...

Page 58: ...edge of the window Spot check several points on the recommended downflow velocity Step 4 Press or button of supply duty cycle to adjust blower speed The objective of this spot check is to obtain the d...

Page 59: ...t Setback Calibration Optional The night set back calibration is performed within the calibration mode after the inflow and downflow calibration procedure is complete NOTE If night setback exhaust air...

Page 60: ...ent method The goal is to reduce the inflow volume to the following values which represents approximately 100 fpm 51m s inflow or may be set at any other values within the damper operational range of...

Page 61: ...t filter is checked using a gross leak method since the exhaust filter is not easily scanned when connected to an exhaust system It may be leak tested by drilling a hole in the duct at a downstream lo...

Page 62: ...low the wiper gasket The criteria used to evaluate the smoke patterns is the following 1 The smoke inside the cabinet shall show smooth downward flow with no dead spots or reflux 2 No smoke shall esca...

Page 63: ...st using non hazardous materials A minimum of three 3 1 minute particle counts shall be sampled and recorded while the user simulates aseptic compounding manipulations At Rest Particle Count Test Take...

Page 64: ...r should be turned on thus the inflow volume balanced with the downflow volume can be measured to set the desired average inflow velocity The inflow exhaust volume is measured by using a Direct Inflow...

Page 65: ...3 8 1263 53 89 6 1369 58 05 4 1475 62 21 2 1580 66 37 5 1686 Number of Readings Average Velocity of Constricted Area ft min mps 1 Average Velocity of Constricted Area fpm m s 2 X Constricted Access Ar...

Page 66: ...e from the table for the nominal average velocity the calibrated exhaust value entered should be 740cfm 1257CMH NOTE 2 For additional guidance the values provided below with a constant 60fpm 30mls dow...

Page 67: ...AG FUSE SIZE 1 4 X 1 1 4 INCH 5 X 20MM 5 X 20MM NU 565 400 10 AMPS 3 AMPS 1 AMP NU 565 600 10 AMPS 3 AMPS 1 AMP 7 10 3 Main Control Board Replacement Note All setup and calibration data will be lost t...

Page 68: ...rror in operation Cabinet type motor type and calibration data will not be affected with this reset Master Reset Resets all calibration cabinet type motor type sensor data and options settings back to...

Page 69: ...OM0301 August 20 Page 69 of 105 Select desired function from menu Perform either reset function as selected below...

Page 70: ...screen will revert back to the Nuaire logo main menu At this point the cabinet MUST be turned off to complete the process Either unplug the cabinet or use the power switch within the control center t...

Page 71: ...ey matches push in and turn the locking ring clockwise until ring locks Proceed to calibrate sensor menu 7 11 2 Exhaust Pressure Sensor The exhaust pressure sensor is a digital differential velocity p...

Page 72: ...arm will be on for 30 seconds upon initial alarm condition then once every ten seconds When presented with an error message please perform the following Step 1 NOTE ALL ERROR MESSAGES Error message wi...

Page 73: ...verride is activated preventing the usage of the cabinet Power Loss Alert Indicates a power interruption has occurred Press DISPLAY to clear message Cabinet outlets won t turn on Check outlet fuse loc...

Page 74: ...s Calibration Service parameter Entry into the Calibration Service functions requires a service password for entry After pressing the Calibration Service menu item a password screen will appear The de...

Page 75: ...ust 20 Page 75 of 105 8 2 2 Calibration Service Menu The Calibration Service menu provides a list of sub menu items to accomplish all service tasks Each sub menu item will be described in the followin...

Page 76: ...control is on the downflow and inflow displays will indicate nominal setpoints Airflow adjustments can be made in the manual mode by going into airflow calibration and adjusting the blower duty cycle...

Page 77: ...arameter allows for the selection of time programmable from 30 to 240 seconds determining how often the exhaust controller updates the control signal to the automatic damper i e NU 951 012 if installe...

Page 78: ...ing a constant exhaust alarm when the exhaust system is turned off Valid settings for the exhaust control damper position are open close freeze or off Off keeps the exhaust control alarms active when...

Page 79: ...damper function will close during a remote override contact closure However this option allows the opposite damper functio 8 2 2 2 Service The service setup menu allows a QUALIFIED TECHNICIAN to conf...

Page 80: ...eset both UV light and motor blower run timer Select desired run time parameter from menu UV Run Time Quantity of hours the UV light has logged ON Reset time for UV lamp replacement Motor Blower Run T...

Page 81: ...feature to indicate an advance notice that re certification is due Press DUE to enter into the certification required time period screen Select desired interval of certification required i e No Remin...

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Page 83: ...et the filter status data used to predict filter life availability Filter status is based on maximum RPM minus the starting RPM entered by technician then scaled to current RPM to determine filter per...

Page 84: ...t conditions as shown Alarm Setup Alarm Silence This parameter allows for the selection of the alarm silence key function When the alarm silence function is on all current and future alarms will be si...

Page 85: ...be silenced The time is displayed in minutes with a programmable range of 1 to 60 Downflow Alarm Time This parameter allows for the selection of time to determine how many continuous seconds of an ala...

Page 86: ...OM0301 August 20 Page 86 of 105 Interlock Features All interlocks are enable disabled by a user through the display...

Page 87: ...HLINK system UV window interlock there is a double redundant UV window interlock relay To override the UV window interlock for service purposes only both interlocks must be changed through the TOUCHLI...

Page 88: ...switch is pending or blue the fan relay will be on or energized In either case the fan relay will be on when the blower switch is on or green and off when the blower switch is off or not colored Blowe...

Page 89: ...his menu item provides the access to aux relay function and accessory outlet functions Aux Relay function This parameter allows for the selection of the AUX relay function When the AUX relay is on the...

Page 90: ...has its own menu screen to excise the control system Select menu screen as desired Blower Sensor Diagnostics Test outputs allow a service technician to exercise these output functions Pressing TOGGLE...

Page 91: ...tic Read inputs all a service technician to exercise or check these input functions The inputs may be checked by altering the state of the input function i e sliding window position and monitoring the...

Page 92: ...utes and then remove This action will cause the cabinet to go into a downflow alarm condition The exhaust airflow reading should increase during this test However the downflow reading should go down b...

Page 93: ...to aid the supply On Off and exhaust delay options The relay will activate whenever the blower is turned on and stay on unless after 5 minutes there is a low exhaust alarm then the relay will de acti...

Page 94: ...that personnel leave the cabinet face area 10 1 2 Operation The operation of the ultraviolet light is accomplished by closing the sliding window and pressing the UV switch location on the front panel...

Page 95: ...p hermolyticus alpha type 5 500 Staphylococcus aureus 6 600 Staphylococcus albus 5 720 Micrococcus sphaeroides 15 400 Sarcina lutea 26 400 Pseudomonas fluorescens 7 040 Escherichia coli 7 040 Proteus...

Page 96: ...h laboratories and most industrial laboratories the expected transient over voltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 11 5 Pollution Degree 2 0 Pollution degree describes the...

Page 97: ...Steel Worksurface Stainless Steel Window Faring Stainless Steel Window Glides HDPE Window Safety Glass Window Frame Stainless Steel Front Service Panel Painted Steel Front Decorative Panel Painted Ste...

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