NPI SEC-05LX, Operating Instructions Manual

Page: 48 / 69

Passive Cell Model User Manual
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version 4.0
page 4
Simulation of electrode in the bath

Set switch # 4 , Figure 1 to the lower position.

Set switch # 5 , Figure 1 to GROUND position. The 1 k

resistor simulates the resistance
of the bath solution. This can be used to train cancellation of offsets, using the bridge
balance and using the capacity compensation.
Simulation of SEAL formation

Set switch # 4 , Figure 1 to the lower position.

Set switch # 5 , Figure 1 to SEAL position. The 1 G

resistor simulates the SEAL
resistance when forming a GIGASEAL in patch clamp experiments.
Simulation of intracellular recording
Intracellular recordings can be mimicked with one of two cells with different properties. Use
the 100 M

electrode connector (# 1 , Figure 1) for an experiment with sharp electrodes or the
5 M

electrode connector (# 3 , Figure 1) for simulating an experiment with patch electrodes.

Switch the CELL membrane switch (see # 4 , Figure 1) to the desired position (CELL 1 or
CELL 2).

Turn all controls at the amplifier to low values (less than 1) and the OFFSET in the range
of 5 (zero position) and the OSCILLATION SHUTOFF in the DISABLED position.

Turn POWER switch of the amplifier on.
Now you can adjust the amplifier (see below) and apply test pulses to the cell model. The
upper position the CELL membrane switch (CELL 1) simulates a cell with a resistance of
100 M

and a capacitance of 100 pF. In the lower position (CELL 2) a cell membrane with
500 M

and 22 pF is simulated.