Molecular Devices SpectraMax Mini User Manual Download Page 1

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5090339 A
March 2022

SpectraMax Mini

Multi-Mode Microplate Reader

User Guide

Summary of Contents for SpectraMax Mini

Page 1: ...5090339 A March 2022 SpectraMax Mini Multi Mode Microplate Reader User Guide ...

Page 2: ...nse to use or permit others to use such manufacturers and or their product names as trademarks Each product is shipped with documentation stating specifications and other technical information Molecular Devices products are warranted to meet the stated specifications Molecular Devices makes no other warranties or representations express or implied including but not limited to the fitness of this p...

Page 3: ...s 19 Chapter 3 Getting Started 23 Plate Drawer 23 Chapter 4 Operating the Instrument 25 Temperature Settings 25 Shake Settings 25 Wavelength Settings 25 Read Plates 26 Opening the Drawer Manually 26 Chapter 5 Read Modes and Read Types 27 Absorbance Read Mode 27 Fluorescence Intensity Read Mode 30 Luminescence Read Mode 32 Read Types 33 Chapter 6 Maintenance 35 Cleaning the Instrument 35 Storing or...

Page 4: ...manner not specified by Molecular Devices the protection provided by the equipment might be impaired Warnings Cautions Notes and Tips All warning symbols are framed within a yellow triangle An exclamation mark is used for most warnings Follow the related safety information Other symbols can warn of other types of hazards such as biohazard or electrical warnings as are described in the text of the ...

Page 5: ...o part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in...

Page 6: ...E Directive of the European Union It indicates that you must not discard this electrical or electronic product or its components in domestic household waste or in the municipal waste collection system For products under the requirement of the WEEE directive contact your dealer or local Molecular Devices office for the procedures to facilitate the proper collection treatment recovery recycling and ...

Page 7: ...or radioactive materials in or near a powered up instrument WARNING Never use the instrument in an environment where potentially damaging liquids or gases are present Electrical Safety To prevent electrical injuries and property damage inspect all electrical equipment before use and report all electrical deficiencies Contact Molecular Devices technical support for equipment service that requires t...

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Page 9: ...scence detection is from 10 6 to 10 12 molar fluorescein Internal compensation for detector sensitivity photomultiplier tube voltage and sensitivity as well as excitation intensity provides a wide dynamic range without PMT gain adjustment The photometric range for absorbance detection is 0 4 ODs with a resolution of 0 001 OD The flexibility and high sensitivity makes this instrument appropriate fo...

Page 10: ...atistical data analysis You should install the SoftMax Pro Software on the computer before you set up the instrument Please be aware that some updates to the SoftMax Pro Software require a purchase Contact Molecular Devices before you update the software For information about the computer specifications required to run the software the software installation and licensing instructions and the direc...

Page 11: ...s for well size spacing and distance from the plate edge Use polystyrene plates for absorbance wavelengths above 340 nm To allow transmission of the deep UV spectra when you do a read at wavelengths below 340 nm you must use special UV transparent plates or quartz plates Endpoint Kinetic and Well Scan read types are available with any plate model For low volume measurements the instrument supports...

Page 12: ...45 Excitation filters on the fluorescence filter cubes pass only the wavelength of light required for excitation Emission filters on the fluorescence filter cubes separate fluorescence generated by the sample from background light and for some luminescence measurements Use the Luminescence filter cube for luminescence reads The filter cubes contain an NFC tag that the instrument reads to determine...

Page 13: ...Hex wrench for transport lock removal Up to four filter cubes depending on how many you purchased Additional filter cubes are shipped in a separate box For a complete list of the package contents see the enclosed packing list Note You must install SoftMax Pro Software version 7 1 2 or higher before you can install the SpectraMax Mini software patch See the SoftMax Pro Data Acquisition and Analysis...

Page 14: ... the cardboard handles below the top layer of foam packing material and slide the accessories package out of the carton This contains the power supply power cord USB cable and a case that contains the filter cubes and other accessories 3 Remove the foam packing material that was above the accessories ...

Page 15: ... the instrument and slide the instrument out of the carton 5 Lift the foam packing material above the instrument upward to reveal the top of the instrument 6 Slide the five vertical pieces of foam packing material that surround the instrument upward to clear the sides of the instrument ...

Page 16: ...rdboard slides and foam packing for future shipments Removing the Transport Lock CAUTION The instrument can be damaged if you do not remove the transport lock before you power on the instrument CAUTION Do not touch or loosen screws or parts other than those specifically designated in the instructions Doing so could cause misalignment and possibly void the warranty ...

Page 17: ...to open the door Hold the door open while you remove the transport lock Note Do not tear the yellow tab It remains attached to the transport lock to make it easier to open the plate chamber door Item Description 1 Screw 1 fastens the lock to the internal frame of the instrument remove first 2 Screw 2 fastens the lock to the plate drawer 3 Screw 3 fastens the lock to the plate drawer 4 Plate drawer...

Page 18: ...er back inside the instrument and close the chamber door Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument Note Before you connect or disconnect the power cord make sure that the power switch that is on the rear of the instrument is in the Off position To connect the instrument cables 1 Turn the instrument around so that the rear of the in...

Page 19: ...e filter chamber on the front of the instrument Each filter cube has an NFC tag that the instrument reads The NFC tag contains the information the instrument uses for reads Filter information displays in the Instrument Information dialog in the software To install filter cubes 1 Turn on the instrument power switch located on the rear of the instrument Wait for the instrument to complete its diagno...

Page 20: ... pull down to open the filter chamber Note The filter chamber should open with no resistance Do not use excessive force to open the chamber 4 If there is a filter cube in the instrument grab the filter cube stem and pull the cube out of the chamber A magnet holds the cube in place You must remove the existing filter cube before you insert a different filter cube ...

Page 21: ...lter chamber A magnet draws the filter cube into place and holds the filter cube in the correct position within the instrument 6 Close the filter chamber cover 7 In the SoftMax Pro Software on the Operations tab in the Ribbon click Info to display the Instrument Information dialog to confirm successful filter cube installation Click OK to exit the Instrument Information dialog ...

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Page 23: ...ouch the internal optic mirrors lenses filters or cables The optics are extremely delicate and critical to the function of the instrument Plate Drawer The plate drawer is located on the front of the instrument and slides in and out of the plate chamber You can use 6 12 24 48 96 and 384 well plates that conform to ANSI SBS standard microplate footprint and dimensions in the instrument See Supported...

Page 24: ...er does not open you can open the drawer manually See Opening the Drawer Manually on page 26 CAUTION To prevent damage to the instrument For the absorbance read mode the plate height must not exceed 22 mm including the lid if the plate is lidded For the fluorescence read mode and the luminescence read mode the plate height must not exceed 20 mm including the lid if the plate is lidded ...

Page 25: ...llows you to define how to shake the plate as part of the read For the Endpoint Spectrum and Well Scan read types you can shake the plate for 0 to 999 seconds before the read at each wavelength you set For the Kinetic read type you can shake the plate for 0 to 999 seconds before the initial read and or before each subsequent read There are three shake speeds Low Medium High There are three shake m...

Page 26: ...Tree and then click Acquisition Settings to display the Settings dialog Select the Wavelength category if needed to confirm the wavelength and click OK to exit the Settings dialog In the Ribbon select the Operations tab and click Info to display the Instrument Information dialog to confirm the filter is correct Click OK to exit the Instrument Information dialog 8 Click Read to read the plate The i...

Page 27: ... use an empty well for a blank Some applications are designed for turbid samples such as algae or other micro organisms in suspension The reported OD values for turbid samples are likely to be different when read by different instruments For optimal results you should run replicates for all blanks controls and samples In this case the blank value that will be subtracted is the average value of all...

Page 28: ...n microplate readers and spectrophotometers The standard pathlength of a 1 cm cuvette is the conventional basis to quantify the unique absorptivity properties of compounds in solution Quantitative analysis can be done on the basis of extinction coefficients without standard curves for example NADH based enzyme assays When you use a cuvette the pathlength is known and is independent of sample volum...

Page 29: ...ot depend on the PathCheck technology to correct for variability in volumes To use a plate blank 1 Designate a minimum of one well preferably several as Plate Blank 2 Pipette buffer for example your sample matrix into those wells and read along with the samples Do not use an empty well for a blank The instrument automatically subtracts the average of the blank wells from each of the samples The OD...

Page 30: ...as ethanol and methanol do not absorb in the NIR region so the solvents do not interfere except to cause a decrease in the water absorbance to the extent of their presence in the solution If the solvent absorbs between 900 nm and 1000 nm the interference would be similar to the interference of highly colored samples If you add an organic solvent other than ethanol or methanol you should run a Spec...

Page 31: ...times greater than that of the emitted light you must have sufficient spectral separation to reduce the interference of the excitation light with detection of the emitted light Tip If the Stokes shift is small you should choose an excitation wavelength that is as far away from the emission maximum as possible while still able to excite the fluorophore so that less of the excited light overlaps the...

Page 32: ...ence intensity of the label which is attached to the compound Such methods are often used to quantify low concentrations of DNA or RNA for example Luminescence Read Mode Luminescence is the emission of light by processes that derive energy from essentially non thermal changes the motion of subatomic particles or the excitation of an atomic system by radiation Luminescence detection relies on the p...

Page 33: ...en 1 ms and 1500 ms Typical luminescence assays require between 500 ms and 1000 ms integration If wells are incubated for a long period of time you should mix the plate before the read This can be done using the Shake setting If it seems that the signal is always higher in the first wells read for example column A you should dark adapt the plate to reduce the auto luminescence of the white plastic...

Page 34: ...allowed values from 200 nm to 1000 nm Well Scan The Well Scan read type takes reads at more than one location on an evenly spaced user defined grid or other pattern inside of each well at single or multiple wavelengths The fill pattern is either round or square to match the well shape The number of points that are available depend on the well size of the plate you select The Read Order you select ...

Page 35: ...ures that could involve contact with either hazardous or biohazardous materials or fluids Do the following before you clean equipment that has been exposed to hazardous material Contact the applicable Chemical and Biological Safety personnel Review the Chemical and Biological Safety information contained in this guide See Chemical and Biological Safety on page 7 Always turn the power switch off an...

Page 36: ...ransport lock to the plate drawer 4 Plate drawer 5 Plate chamber door in open position 6 Plate drawer transport lock 5 Manually open the plate chamber door gently slide the plate drawer out of the instrument and place the transport lock on the end of the of the plate drawer 6 Use the hex key included in the accessory case to tighten screws 2 and 3 until the transport lock is attached to the plate ...

Page 37: ... open on the side place the bottom cardboard slide at the opening with the handles oriented away from the carton 13 Place the bottom foam packing material onto the cardboard slide with the medium insert slot oriented towards the cardboard carton see following images 14 Place the instrument onto the bottom foam packaging material with the rear of the instrument aligned with the medium insert slot t...

Page 38: ...the instrument and the medium vertical foam packing material at the rear of the instrument 16 Place the small vertical foam packing material with the slot in front of the instrument power button and the other small vertical foam packing material on the other side of the filter door in the front of the instrument ...

Page 39: ...the top foam packing material and place it over the vertical foam packing material 18 Fold the handles on the cardboard slide upward and use the cardboard to slide the instrument into the carton 19 Place the accessories into the upper foam packaging material ...

Page 40: ...second cardboard slide fold the handles upward and slide it into the carton above the instrument 21 Slide the top foam packing material into the carton above the accessories 22 If needed turn the box upright to ensure that all items are settled inside the carton 23 Seal the carton with packing tape ...

Page 41: ...ge 200 1000 nm Wavelength Selection Monochromator tunable in 1 nm increments Wavelength Bandwidth 5 0 nm full width half maximum Wavelength Accuracy 2 nm across wavelength range Wavelength Repeatability 0 2 nm Dynamic Range 0 to 4 OD Photometric Resolution 0 001 OD Photometric Accuracy Linearity 0 006 OD 1 0 0 3 OD Photometric Precision 0 003 OD 1 0 0 3 OD Stray Light 0 05 at 230 nm Light Source X...

Page 42: ...o achieve temperature regulation at 45 C the ambient temperature must be 20 C Resolution Resolution 0 1 C Accuracy 2 0 C for plate chamber Temperature uniformity at equilibrium 1 C at 37 C Chamber warmup time 30 50 minutes measured on air after initiation of temperature regulation Temperature regulation 2 sensors Drift 0 2 C regulated Temperature regulation diagnostics Temperature regulation syste...

Page 43: ... 32 cm W x 35 cm H x 40 cm D 12 6 in W x 13 8 in H x 15 7 in D Rear clearance 20 cm to 30 cm 7 9 in to 11 8 in between the rear of the instrument and the wall for ventilation and cable disconnects Weight 18 kg 40 lbs Plate formats 6 12 24 48 96 384 well plates ANSI SLAS conformant Absorbance read mode Maximum height 22 mm Fluorescence read mode and luminescence read mode Maximum height 20 mm Readi...

Page 44: ...hz Multi Standard Multi Antenna Reader Writer Contains FCC ID 2AKHW SANGMSMA4 Contains IC 22202 SANGMSMA4 Changes or modifications made to this equipment not expressly approved by the party responsible for compliance may void the FCC authorization to operate this equipment Physical Specifications continued Electromagnetic Compatibility Regulatory Information for Canada ICES NMB 001 2020 This ISM d...

Page 45: ...301193 Obtaining Support Molecular Devices is a leading worldwide manufacturer and distributor of analytical instrumentation software and reagents We are committed to the quality of our products and to fully supporting our customers with the highest level of technical service Our Support website support moleculardevices com has a link to the Knowledge Base which contains technical notes software u...

Page 46: ...operty of Molecular Devices LLC or their respective owners Specifications subject to change without notice Patents www moleculardevices com patents FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PROCEDURES 2022 Molecular Devices LLC All rights reserved 5090339 A ...

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