5090339 A
41
Appendix A: Specifications
Thermal specifications for plates used in the instrument apply to flat-bottom plates with isolated
wells. All other plate specifications apply to standard 96-well polystyrene flat-bottom plates.
Note: Technical specifications are subject to change without notice. Molecular Devices
provides validation documentation for software and hardware, as well as absorbance
and fluorescence detection test tools with its SpectraTest® solutions. The SpectraTest
line of microplate reader validation packages provide automated and comprehensive
validation of a microplate reader's optical performance.
Item
Description
Wavelength Range
200-1000 nm
Wavelength Selection
Monochromator, tunable in 1 nm increments
Wavelength Bandwidth
≤
5.0 nm full width half maximum
Wavelength Accuracy
2 nm across wavelength range
Wavelength Repeatability
±0.2 nm
Dynamic Range
0 to 4 OD
Photometric Resolution
0.001 OD
Photometric Accuracy Linearity
< ± 0.006 OD ± 1.0%, 0-3 OD
Photometric Precision
< ± 0.003 OD ± 1.0%, 0-3 OD
Stray Light
≤
0.05% at 230 nm
Light Source
Xenon flash lamp (5 Watts)
Absorbance Photometric Performance
Item
Description
Filter Based
Top Read
Dynamic Range
> 6 Decades
Sensitivity: Fluorescein
1 pM
Excitation Wavelength and
Emission Wavelength Range
For available Fluorescence filter cubes, see
Adjustable Read Height
Z range from upper plate @ 1 mm to max height 15.98 mm
Read Time
30 seconds 96-well plate
96 seconds 384-well plate
Fluorescence Intensity Performance
A
