Molecular Devices AquaMax 2000, User Manual

Page: 48 / 158

AquaMax
®
2000/4000 Microplate Washer User Guide
48 0112-0151 K
4 Volume–Volume to be dispensed.
Use 5 to 150
μ L for 384-well plates and 20 to 400 μ L for 96-well
plates. The volume must be within the specified range for the plate or
the program cannot be saved or run.
Note: You should never dispense a greater volume than is
listed by the plate manufacturer.
Table 4-9: Plate Wash and Cell Wash Dispense Rates
Plate wash
dispense
rate
96 plate wash 384 plate wash
(mmHg) ( μ L/plate/sec) (mmHg) ( μ L/plate/sec)
1 150 18300 150 21600
2 200 22100 200 25600
3 250 25400 250 26200
4 350 31300 350 35600
5 450 36600 450 41400
Cell wash
dispense
rate
96 cell wash 384 cell wash
(mmHg) ( μ L/plate/sec) (mmHg) ( μ L/plate/sec)
1 75 7200 150 19400
2 100 9100 200 23000
3 150 12400 250 26300
4 250 17800 350 32100
5 350 22500 450 37400
Table 4-8: Dispense settings
Item Description
Tip: Best dispense volume accuracy and precision are achieved at the
highest rate (Rate 5) and volume.