4.2 Checking the intensity spectrum
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
40
￭￭￭￭￭￭￭￭
947 Professional UV/VIS Detector Vario (2.947.00x0)
￭
The lenses and the flow path of the flow-through cell must be clean.
1 Rinsing the flow-through cell with ultrapure water
Rinse the flow-through cell with ultrapure water at a flow rate of 0.5
mL/min.
Ensure that no air bubbles remain in the flow-through cell.
2 Checking the intensity of the lamps
In MagIC Net, adjust the following settings:
￭
Load and start a method with UV/VIS detection in the
Work-
place
program part.
￭
Go to the
Manual
program part.
￭
Click on the
icon for the
947 UV/VIS Detector
.
￭
On the
UV lamp
tab, switch on the UV lamp.
￭
On the
VIS lamp
tab, switch on the VIS lamp.
￭
On the
Detector
tab, select the
Intensity spectrum
.
First click on
[Reset baseline]
and then click on
[View]
.
The lamp's intensity range is monitored and the spectrum is
recorded.
If the intensity spectrum looks similar to the spectrum in figure
Intensity
spectrum OK
, then the lamp is correctly adjusted.
Figure 29
Intensity spectrum OK
If the intensity spectrum is cut off at the top like the one in figure
Lamp
intensity too high
, then the lamp settings must be adjusted
.
Summary of Contents for 2.947.0010
Page 1: ...947 Professional UV VIS Detector Vario 2 947 00x0 Manual 8 0947 8001EN 2020 06 30 ...
Page 2: ......
Page 8: ......
