Metrohm 2.792.0020 Basic IC, Instructions For Use Manual, Page: 105 / 197

4 Operation
792 Basic IC
96
External standard calibration
External standard calibration (absolute calibration) is a key way of cali-
bration. Basically this procedure calculates the dependence of injected
component's quantity versus area (or height) of the corresponding
peak. This dependence is showed as a calibration plot with injected
quantity along the Y axis and peak area (or height) along the X axis. The
injected quantity
Q
i
is calculated as product of component's concentra-
tion
C
i
to the reduced injected sample volume
V'
:
Q
i
= C
i
•
V'
The calibration function
W
i
(R)
for each component is calculated using
the RSD method.
W
i
(R) = RSD(Q
ij
, R
ij
) = RSD((C
i
•
V'), R
ij
)
When quantification procedure is performed, a concentration
C
i
(abso-
lute concentration of a component in the sample) is determined as a ra-
tio of the computed quantity
W
i
(R
i
)
to the corrected volume of sample
injected
V'
:
C
i
= W
i
(R
i
) / V'
Component table
792 BASIC IC / Method / Calibration / Components
If the component table is opened, the chromatogram window is split
into two parts. The upper one shows the chromatogram, the compo-
nent table appears in the bottom part of the screen. When moving into
the component table, a special cursor in the upper part of the window
jumps to the peak corresponding to the current component.
The
Components table
stores the following information on the compo-
nents to be analyzed: