Plasmid DNA Purification
MACHEREY-NAGEL – 01/2008/ Rev. 04
19
0
10
20
30
40
50
60
70
80
90
100
0
200
400
600
800
1000
Elution Volume [
l]
Recovery [%]
0,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
Concentration [
g/
l]
Recovery
Concentration
Figure 4
Final DNA recovery and concentration after NucleoBond
®
Finalizer application
A NucleoBond
®
Xtra Midi eluate containing 250 µg plasmid DNA (8 kbp) was loaded onto a
NucleoBond
®
Finalizer and eluted two-fold with increasing volumes of TE buffer.
The
NucleoBond
®
Finalizer
is designed to hold a maximum of 500 µg DNA and is
therefore ideally suited to be used in combination with
NucleoBond
®
Xtra Midi
.
Maximum DNA recovery can be achieved by using > 600 µl of elution buffer. For a
higher concentration experienced users can lower the elution buffer volume to 400-
200 µl.
Table 4 gives an overview about recovery and concentration of different amounts of
plasmid DNA loaded onto a
NucleoBond
®
Finalizer
. DNA was eluted two-fold with
increasing volumes of TE-EF. Please refer to this tables to select an elution buffer
volume that meets your needs best.
Table 4: DNA recovery and concentration for the NucleoBond
®
Finalizer
Elution Volume
100 µl
200 µl
400 µl
600 µl
800 µl
1000 µl
35 %
60 %
70 %
75 %
75 %
75 %
500 µg
2.5 µg/µl
2.3 µg/µl
1.2 µg/µl
0.8 µg/µl
0.6 µg/µl
0.5 µg/µl
40 %
65 %
75 %
80 %
80 %
80 %
250 µg
1.9 µg/µl
1.1 µg/µl
0. 6 µg/µl
0.4 µg/µl
0.3 µg/µl
0.2 µg/µl
45 %
70 %
80 %
85 %
85 %
85 %
100 µg
0.7 µg/µl
0.4 µg/µl
0.2 µg/µl
0.1 µg/µl
0.1 µg/µl
0.1 µg/µl
30 %
75 %
85 %
90 %
90 %
90 %
Loaded DNA
50 µg
0.3 µg/µl
0.2 µg/µl
0.1 µg/µl
0.1 µg/µl
0.1 µg/µl
<0.1 µg/µl
DNA recovery
DNA concentration
