Plasmid DNA Purification
MACHEREY-NAGEL – 01/2008/ Rev. 04
16
4.8
Setup of NucleoBond
®
Xtra columns
Ideally the
NucleoBond
®
Xtra Midi
or
Maxi
Columns
are placed into a
N u-
cleoBond
®
Xtra Combi Rack
(see section 8.2 for ordering information). They are
held either by the collar ring of the cartridges or by the Plastic Washers included in
the kit to individually adjust the height of each column (see Figure 3). The Plastic
Washers can also be used to hold the columns on top of suitable collecting tubes or
flasks. The
NucleoBond
®
Xtra Combi Rack
can be used in combination with
Nu-
cleoBond
®
PC 100, 500,
and
2000
as well. Note that the
NucleoBond
®
Xtra
Midi
Columns
can also be placed in the
NucleoBond
®
Rack Large
(Cat. No. 740563).
A
B
Figure 3
Setup of NucleoBond
®
Xtra Midi/Maxi Columns with the NucleoBond
®
Xtra Combi Rack
A: Setup for clarification, loading, and first washing step, B: Setup for elution.
