4
BioResearch
HT Nucleofector™ hardware manual
1 The Amaxa™ Nucleofector™ technology
Since its introduction in 2001, Amaxa™ Nucleofector™ technology has
transformed transfection. Nucleofection™ has proven to be a reliable and
reproducible easy-to-use technology that is suited to a wide variety of
applications and provides an answer to almost every transfection
challenge. An ever-increasing number of publications in a wide range of
research areas reflect how Nucleofector™ technology is driving research
in numerous cell types and applications.
How it works
Nucleofector™ technology is based on two unique components: the
Nucleofector™ system, which delivers the specifically optimized electrical
parameters and Nucleofector™ kits, which contain specific Nucleofector™
solutions. As part of Nucleofector™ technology, Lonza provides cell-type
specific optimized protocols for many different cell lines and primary cells.
Transfection of any cell
With over 160 protocols for cell lines and primary cells optimized by
Lonza’s R&D team and more than 680 entries in the online cell data base,
Nucleofector™ technology is clearly the transfection method-of-choice
for any difficult-to-transfect cell type. Moreover, Nucleofection™ is the
only electroporation based technology allowing reliable transfection of
adherent cells and cells in suspension.
Transfection of any substrate
Nucleofector™ technology offers high flexibility within applications,
since the same transfection parameters apply for almost all substrates.
DNA vectors, RNA duplexes, and peptides can be transfected using our
sophisticated transfection protocols. This makes Nucleofection™ the
ideal tool for providing answers to scientific questions in over expression
studies, gene silencing approaches, protein expression, generation of
stable clones and many more applications.
www.lonza.com / cell-database
