11
2.7 Instructions for using the
HT Nucleofector™ system
The table below gives a brief overview of the steps required for a typical
Nucleofection™ workflow.
Step
Description
For more information see
1
Switch on the HT Nucleofector™ system.
This chapter
2
Start the HT Nucleofector™ software on the computer and log in.
Software manual
3
Check your user settings (optional).
Software manual
4
Check that all connections are active / reconnected
Software manual
5
Open an existing experiment or create a new experiment.
Software manual
6
Prepare and transfer samples into the 384-well Nucleocuvette™ plate.
Optimized protocol
7
Insert the 384-well Nucleocuvette™ plate into the HT Nucleofector™ plate handler.
Chapter 3.1
8
Start Nucleofection™ process from the computer.
Software manual
9
Monitor Nucleofection™ process during program execution (optional).
Software manual
10
Remove 384-well Nucleocuvette™ plate from the plate handler and transfer samples to
appropriate culture plates.
Optimized protocol
11
Check result file on the computer for potential errors.
Software manual
12
Close files, exit HT Nucleofector™ software and switch off all components.
Software manual
The HT Nucleofector™ system enables Nucleofection™ experiments to be
conveniently controlled and monitored from a computer. Only a few steps
are required to initialize the system.
– Switch on the HT Nucleofector™ system via the main switch located
on the front panel of the power supply. LED 1 (green) on the plate
handler front panel is lit.
– Start the HT Nucleofector™ software on the personal computer and
log in. LED 2 (green) on the plate handler front panel blinks during
the boot sequence and remains lit when the process is successfully
completed. The system is now initialized.
– If LED 3 (red) is lit, an error occurred during the boot process. Refer to
the HT Nucleofector™ software manual for troubleshooting.
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