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BioResearch
HT Nucleofector™ hardware manual
3 Running an experiment
This chapter explains how to run a Nucleofection™ experiment on the
HT Nucleofector™ system. Because correct cell handling before and
after the Nucleofection™ process is crucial for successful experiments,
refer to the dedicated optimized protocol to find detailed guidelines for
these steps.
3.1 Processing a 384-well
Nucleocuvette™ plate
This chapter describes loading and processing of the 384-well
Nucleocuvette™ plate using a predefined experiment. See the
HT Nucleofector™ software manual for further details on defining and
modifying experiments.
Figure 3.1: 384-well Nucleocuvette™ plate handler work area
The plate handler carousel has two plate retainer positions for
384-well Nucleocuvette™ plates. Load plates into the retainer position
that is accessible at the front of the plate handler. Do
not
turn the plate
handler carousel manually to access the second position. To load
prepared plates:
– Make sure that the plate is covered with an appropriate lid.
– Make sure that plate position A01 is in the upper left corner.
– Place the plate gently into the plate handler retainer position (1)
After loading the 384-well Nucleocuvette™ plate, start the experiment from
the computer. The 384-well Nucleocuvette™ plate will be automatically
rotated into the plate handler to be processed. It will be rotated back when
the experiment is completed. You can follow the progress of the experiment
by checking the blue progress LEDs on the front panel of the plate handler.
If the HT Nucleofector™ is integrated into an automated liquid handling
system follow the appropriate instructions in the HT Nucleofector™
software manual for plate handling.
NOTE: Do not attempt to turn the carousel manually when the system is active! This will
compromise system integrity.
NOTE: Do not use lids other than those provided in the 384-well Nucleofector™ kits. Other
lids may cause damage to the HT Nucleofector™ plate handler.
After completion of a run, remove the processed 384-well Nucleocuvette™
plate from the plate handler and process it according to the relevant
optimized protocol.
3.2 System shutdown
Close all parameter and result files, exit the HT Nucleofector™ operating
software, and switch off the system using the main switch on the
HT Nucleofector™ power supply front panel.
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