Lonza Amaxa HT Nucleofector Hardware Manual Download Page 20

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Lonza Cologne GmbH  – 50829 Cologne, Germany

For research use only. Not for use in diagnostic procedures.
Manufacturer and distributor information: HT Nucleofector™ system 
is manufactured by Lonza Cologne GmbH, Nattermannallee 1, 50829 
Cologne, Germany and distributed in the US by Lonza Walkersville, Inc. 

(8830 Biggs Ford Road, Walkersville, MD 21793).
The use of this product, alone or in combination with materials and/or 
methods of others, may require a license from a third party. User shall 

be fully responsible for determining whether and from whom it requires 

such license and for obtaining such license.
The Amaxa™ Nucleofector™ technology, comprising Nucleofection™ 
process, Nucleofector™ device, Nucleofector™ solutions, Nucleofector™  
96-well Shuttle™ system and Nucleocuvette™ plates and modules 

is covered by patent and/or patent pending rights owned by Lonza 
Cologne GmbH.
Amaxa, Nucleofector, Nucleofection, Nucleocuvette, maxGFP and 96-well 
Shuttle are trademarks of Lonza Cologne GmbH in Germany and / or the 
U.S. and / or other countries.
Other product and company names mentioned herein are the trademarks 

of their respective owners.
Amaxa™ Nucleofector™ kits contain a proprietary nucleic acid coding for a 
proprietary copepod protein fluorescent protein intended to be used as 
a positive control with Lonza products only. Any use of the proprietary 
nucleic acid or protein other than as a positive control with a Lonza 
product is strictly prohibited. Use in any other application requires a 

license from Evrogen. To obtain such a license, please contact Evrogen 

at [email protected].
The information contained herein is believed to be correct and 
corresponds to the latest state of scientific and technical knowledge. 

However, no warranty is made, either expressed or implied, regarding its 

accuracy or the results to be obtained from the use of such information 
and no warranty is expressed or implied concerning the use of these 
products. The buyer assumes all risks of use and/or handling. No 
statement is intended or should be construed as a recommendation to 

infringe any existing patent.

© Copyright 2011, Lonza Cologne GmbH. All rights reserved.  

 CD-MN030 

Contact information

Online ordering

https://shop.lonza.com

Customer service

North America

800 638 8174 (toll free)

Europe

+ 32 87 321 611

International

Contact your local Lonza distributor
+1 301 898 7025, ext. 1500

Scientific support

North America

800 521 0390 (toll-free)
[email protected]

Europe

+49 221 99199 400
[email protected]

International

[email protected]

International offices

Australia 

 

+61 3 9550 0883

Austria  

 

   0800 201 538 (toll free)

Belgium 

 

+32 87 321 611

Brazil   

 

+55 11 5641 3325

Denmark 

 

   808 83 159 (toll free)

France   

 

   0800 91 19 81 (toll free)

Germany 

 

    0800 182 52 87 (toll free)

India   

 

+91 22 4342 4000

Ireland  

 

   1 800 654 253 (toll free)

Italy   

 

   800 789 888 (toll free)

Japan   

 

+81 3 5566 0622

Luxemburg 

 

+32 87 321 611

Poland   

 

+48 781 120 300

Singapore 

 

+65 6521 4379

Spain   

 

   900 963 298 (toll free)

Sweden 

 

   020 790 220 (toll free)

Switzerland 

 

   0800 83 86 20 (toll free)

The Netherlands     0800 022 4525 (toll free)
United Kingdom     0808 234 97 88 (toll free)

Summary of Contents for Amaxa HT Nucleofector

Page 1: ...BioResearch Amaxa HT Nucleofector hardware manual For research use only...

Page 2: ...e non transferable right to use the system as well as Lonza s proprietary Nucleofector technology only for research conducted by the buyer whether the buyer is an academic or for profit entity For fur...

Page 3: ...em components 8 2 6 System set up instructions 10 2 7 Instructions for using the HT Nucleofector system 11 3 Running an experiment 12 3 1 Processing a 384 well Nucleocuvette plate 12 3 2 System shutdo...

Page 4: ...provides cell type specificoptimizedprotocolsformanydifferentcelllinesandprimarycells Transfection of any cell With over 160 protocols for cell lines and primary cells optimized by Lonza sR Dteamandm...

Page 5: ...eofector system meets your screening requirements Experience the benefits of the HT Nucleofector system Automation compatible full integration into liquid handling platforms Fast process 384 samples i...

Page 6: ...esearch conducted by the buyer whetherthebuyerisanacademicorfor profitentity Forfurtherdetails about the license refer to chapter 5 For license information contact Lonza Cologne GmbH Phone 49 221 9919...

Page 7: ...eonprecautionsto be taken before further use Donotopenthedevicehousing Undernocircumstancesshouldcircuit components be interfered with as they can deliver an electric shock even when the system is not...

Page 8: ...fector solutions in a biohazard container Refer to your local waste management organization and to the relevant laboratory safety instructions for proper disposal practices 2 5 HT Nucleofector system...

Page 9: ...upply 7 8 10 9 12 11 HT Nucleofector plate handler The HT Nucleofector plate handler serves as the contacting unit of the system Transfection of the samples occurs in 384 well Nucleocuvette plates tha...

Page 10: ...technical specialist Follow the instructions below if the instrument is moved from its original location ConnecttheHVportoftheHTNucleofector powersupply 10 tothe HVportontheplatehandlerUnit 13 usingth...

Page 11: ...m execution optional Software manual 10 Remove 384 well Nucleocuvette plate from the plate handler and transfer samples to appropriate culture plates Optimized protocol 11 Check result file on the com...

Page 12: ...re that plate position A01 is in the upper left corner Place the plate gently into the plate handler retainer position 1 Afterloadingthe384 wellNucleocuvette plate starttheexperimentfrom the computer...

Page 13: ...this may negatively influence the viability of the cells after Nucleofection For detailed recommendations on passage number cell density and confluency refer to the relevant optimized protocol Low tr...

Page 14: ...tch of the power supply 2 Disconnect the main power cable from the power supply power inlet 3 Remove the small plastic cap on top of the plate handler housing see Figure 4 1 to access the emergency li...

Page 15: ...esentations and warranties Lonza Cologne represents and warrants that it is not aware of any legal deficiencies of the patents and that to its knowledge it has the right to dispose of the patents Lonz...

Page 16: ...50 45 cm 5 3 19 6 17 7 in Manufacturing date The year of manufacture is indicated by the second and third digit of the serial number e g a device with the serial number x11xxxxx was manufactured in 20...

Page 17: ...17 Notes 6...

Page 18: ...18 BioResearch HT Nucleofector hardware manual Notes...

Page 19: ...19 Notes...

Page 20: ...ed Use in any other application requires a license from Evrogen To obtain such a license please contact Evrogen at license evrogen com The information contained herein is believed to be correct and co...

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