Lonza Amaxa HT Nucleofector Hardware Manual Download Page 15

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The purchase of the HT Nucleofector™ conveys to the buyer a non-
transferable, non-exclusive license without the right to grant  
sublicenses, limited for solely the use of Lonza Cologne’s proprietary 
Nucleofector™ process and method.

The license is limited to research use only. The license does not include the 
right to use the purchased device, its components or materials for any type 
whatsoever of commercial purpose as defined below. Notwithstanding 
the foregoing, the buyer shall be allowed to use the licensed process in 
combination with the purchased device to perform services internally for 
affiliates, subsidiaries, sections, departments or scientific collaborators, 
provided that such services are not for any commercial purpose. 

Under no circumstances

 shall the licensed process and/or the purchased 

device be used for testing in or treatment of humans. The buyer 
acknowledges that the licensed process and/or the purchased device are 
not diagnostic or therapeutic tools and that they shall not be used as an  
accessory or complement to such tools.

Commercial purposes means any activity by a party for a financial 
consideration and may include, but is not limited to: (1) use of the 
product or its components in manufacturing; (2) use of the product or 
its components to provide a service, information, or data; (3) use of the 
product or its components for therapeutic, diagnostic or prophylactic 
purposes; or (4) resale of the product or its components, whether or not 
such product or its components are resold for use in research.

The buyer may not manufacture, copy, reproduce, transmit, distribute, sell, 
lease, transfer or sublicense the licensed process and/or the purchased 
device.

 

For information on purchasing a license to this product for purposes  

 

other than research, contact our licensing department:

 

Phone:   + 49 221 99199 0 

 

E-mail: [email protected]

Representations and warranties

Lonza Cologne represents and warrants that, it is not aware of  
any legal deficiencies of the patents and that to its knowledge it has the 
right to dispose of the patents. Lonza Cologne further represents and 
warrants that it has no knowledge of any rights of third parties which have 
priority over the patents and that the inventors of the invention falling 
under the patents have transferred all rights and entitlements to Lonza 
Cologne and that accordingly Lonza Cologne has full power and authority 
to grant the license provided hereunder.

Lonza Cologne does not warrant that the information and data pertaining 
to the licensed process and/or the purchased device is correct and without 
defects, that the use of such information or data is adequate for the use 
of the licensed process and/or the purchased device or that the technical 
information or data for the licensed process and/or the purchased device 
is complete.

Lonza Cologne does not warrant either that the use of the license does not 
infringe third parties’ rights or does not cause damages to third parties. 
Any liability for later invalidation or lapse of the patents is excluded.

Subject to the warranty granted for the purchased device, all warranty 
claims are excluded as far as they are based on the licensed process 
or the purchased device. No warranty is made for the reliability, the  
quality, the economic utilization, efficiency, merchantability, fitness 
for purpose and the usefulness of the licensed process, the purchased 
device, or products resulting from the use of the licensed process or the  
purchased device, for the intended purpose or for any other purpose. The 
exclusion of warranty claims does not apply in case of gross negligence 
or intentional misconduct of Lonza Cologne.

Lonza Cologne does not assume any liability that the licensed process, 
the purchased device or any product resulting from the use of the licensed 
product shall be approved by governmental authorities or any other 
institution or supervisory board or similar agencies.

5  Purchaser notification

5

Summary of Contents for Amaxa HT Nucleofector

Page 1: ...BioResearch Amaxa HT Nucleofector hardware manual For research use only...

Page 2: ...e non transferable right to use the system as well as Lonza s proprietary Nucleofector technology only for research conducted by the buyer whether the buyer is an academic or for profit entity For fur...

Page 3: ...em components 8 2 6 System set up instructions 10 2 7 Instructions for using the HT Nucleofector system 11 3 Running an experiment 12 3 1 Processing a 384 well Nucleocuvette plate 12 3 2 System shutdo...

Page 4: ...provides cell type specificoptimizedprotocolsformanydifferentcelllinesandprimarycells Transfection of any cell With over 160 protocols for cell lines and primary cells optimized by Lonza sR Dteamandm...

Page 5: ...eofector system meets your screening requirements Experience the benefits of the HT Nucleofector system Automation compatible full integration into liquid handling platforms Fast process 384 samples i...

Page 6: ...esearch conducted by the buyer whetherthebuyerisanacademicorfor profitentity Forfurtherdetails about the license refer to chapter 5 For license information contact Lonza Cologne GmbH Phone 49 221 9919...

Page 7: ...eonprecautionsto be taken before further use Donotopenthedevicehousing Undernocircumstancesshouldcircuit components be interfered with as they can deliver an electric shock even when the system is not...

Page 8: ...fector solutions in a biohazard container Refer to your local waste management organization and to the relevant laboratory safety instructions for proper disposal practices 2 5 HT Nucleofector system...

Page 9: ...upply 7 8 10 9 12 11 HT Nucleofector plate handler The HT Nucleofector plate handler serves as the contacting unit of the system Transfection of the samples occurs in 384 well Nucleocuvette plates tha...

Page 10: ...technical specialist Follow the instructions below if the instrument is moved from its original location ConnecttheHVportoftheHTNucleofector powersupply 10 tothe HVportontheplatehandlerUnit 13 usingth...

Page 11: ...m execution optional Software manual 10 Remove 384 well Nucleocuvette plate from the plate handler and transfer samples to appropriate culture plates Optimized protocol 11 Check result file on the com...

Page 12: ...re that plate position A01 is in the upper left corner Place the plate gently into the plate handler retainer position 1 Afterloadingthe384 wellNucleocuvette plate starttheexperimentfrom the computer...

Page 13: ...this may negatively influence the viability of the cells after Nucleofection For detailed recommendations on passage number cell density and confluency refer to the relevant optimized protocol Low tr...

Page 14: ...tch of the power supply 2 Disconnect the main power cable from the power supply power inlet 3 Remove the small plastic cap on top of the plate handler housing see Figure 4 1 to access the emergency li...

Page 15: ...esentations and warranties Lonza Cologne represents and warrants that it is not aware of any legal deficiencies of the patents and that to its knowledge it has the right to dispose of the patents Lonz...

Page 16: ...50 45 cm 5 3 19 6 17 7 in Manufacturing date The year of manufacture is indicated by the second and third digit of the serial number e g a device with the serial number x11xxxxx was manufactured in 20...

Page 17: ...17 Notes 6...

Page 18: ...18 BioResearch HT Nucleofector hardware manual Notes...

Page 19: ...19 Notes...

Page 20: ...ed Use in any other application requires a license from Evrogen To obtain such a license please contact Evrogen at license evrogen com The information contained herein is believed to be correct and co...

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