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BioResearch
HT Nucleofector™ hardware manual
The Nucleofector™ software start screen
The HT Nucleofector™ operating software provides the graphical user
interface for operating the HT Nucleofector™ system. It controls all
processes during an experiment and manages data transfer to the host PC.
Figure 2.6: Login screen
384-well Nucleocuvette™ plate – solution and supplement
High-throughput Nucleofection™ occurs in unique 384-well Nucleocuvette™
plates supplied with HT Nucleofector™ kits. The plates meet SBS standards
and can therefore be used in liquid handling workstations.
Figure 2.7: HT Nucleofector consumables
2.6 System set-up instructions
The HT Nucleofector™ system will be installed and checked by a Lonza
technical specialist. Follow the instructions below if the instrument is
moved from its original location.
– Connect the HV port of the HT Nucleofector™ power supply (10) to the
HV port on the plate handler Unit (13) using the cable marked “Lonza
interface HV cable.”
– Connect the data port of the HT Nucleofector™ power supply (11) to
the data port on the plate handler (17) using the cable marked “Lonza
interface data cable.”
– Connect the USB slave port of the HT Nucleofector™ plate handler (15)
to a USB port on your personal computer using the supplied USB cable.
– If required install, the HT Nucleofector™ operating software on
your personal computer, following the instructions in the HT
Nucleofector™ software manual.
– Connect the main power plug of the HT Nucleofector™ power supply
(8) to a wall outlet with protective grounding conforming to the power
rating specified in Chapter 6.
– Start the system as described in Chapter 3.1 of the HT Nucleofector™
software manual.
