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BioResearch
HT Nucleofector™ hardware manual
Figure 4.1: Plastic cap covering the emergency release of the contact bridge
Figure 4.2:
Lift contact bridge by turning the screw clockwise
Figure 4.3: Turn carousel manually to recover samples
4.2 Mechanical failures
Great care has been taken to ensure that mechanical failures will not occur
as long as the system is operated using the guidelines presented in this
manual and in the HT Nucleofector™ software manual.
In case of a system failure while a 384-well Nucleocuvette™ plate is still
inside the plate handler unit, proceed as follows to recover your samples:
1. Turn off the HT Nucleofector™ using the main switch of the
power supply.
2. Disconnect the main power cable from the power supply power
inlet.
3. Remove the small plastic cap on top of the plate handler housing
(see Figure 4.1) to access the emergency lift screw of the contact
bridge.
4. Using a screwdriver, turn the screw clockwise as far as possible.
5. The plate handler carousel can be turned manually and samples can
be recovered.
6. To recover a 384-well Nucleocuvette™ plate loaded on plate retainer
position 1 (position 2 is visible at the front of the plate handler)
turn the carousel counterclockwise to recover your samples. To
recover samples from plate retainer position 2 (position 1 is
visible), turn the carousel clockwise.
7. Remove the 384-well Nucleocuvette™ plate from the plate handler
carousel.
8. Contact Lonza’s technical service for analyzing the error and for
repair of the system.
