21
Leica VT1000 S – Microtome
-
Fix the knife holder
(1)
with the knife holder clamping screw
(2)
.
-
Use the
REV/FORW
-rocker button to place the knife edge right behind
the rear edge (from user’s view) of the specimen.
-
Pull the
UP/DOWN
-rocker button into the
UP
-direction and keep it in
the
UP
position until the specimen surface is shortly below the level
of the knife edge (see arrow
(3)
).
-
Select sectioning speed and sectioning frequency with the control
knobs
SPEED
and
FREQ
.
-
Use the
+/-
button to select a sectioning thickness for trimming.
-
Select a sectioning range appropriate to the size of the specimen
with the
SECTIONING WINDOW
-button.
-
Switch the
SINGLE/CONT
-button to
CONT
.
Push the
START/STOP
-button.
The instrument will now trim the specimen at the selected trimming
thickness until you push the
START/STOP
button once more.
-
Once you have reached the desired specimen level for sectioning,
use the
+/-
button to select the desired thickness for sectioning..
For sectioning proceed as follows:
-
Select the desired section thickness via the
+/-
button.
-
Switch the
SINGLE/CONT
-button to
SINGLE
.
-
Push the
START/STOP
-button.
The instrument will now produce a section
(4)
. When the section is
finished, the knife will automatically stop at the rear end position be-
hind the specimen (from the user´s view).
-
Pick up the section as shown on the left using a brush
(5)
to mount it
on a glass slide
(6)
.
5.
Operation
Fig. 21.1
Fig. 21.2
1
2
3
4
5
6
Fig. 21.3
