Last Updated on Sep 2021
•
Then select,
Dye assistant
to set up your laser pathway.
1.
Click
to add the number of channels
you would like to image.
2.
Click
to select the dyes you are
using for each channel.
3.
Then select the type of detector (HyD or
PMT) you would like to image with your
dyes. HyD is more sensitive than PMT.
4.
The Dye assistant will give you a list of
laser settings options to select from.
Select the settings which has the least
amount of crosstalk. Sequential Scan is
always preferred as the amount of
crosstalk is lesser. Line sequencing is
much faster than frame/stack imaging.
5.
Select your preferred laser setting option
and click
apply
.
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