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Last Updated on Sep 2021 

allowed to conduct the training. None of your lab members are allowed to conduct the training 
for you.  

If you need a refresher or some specific advice on anything microscopy related, please 
approach any member of the Bioimaging Facility for assistance/help.  

We also offer training on the various types of image analysis and image processing software 
available here at TLL, including ImageJ/Fiji, Huygens and Imaris. 

3. Online Booking and System Access 

 

Booking of all Light microscopes prior to use is 

COMPULSORY 

through

 

microscopy 

resource booking via TLL intranet 

(

https://intranet.tll.org.sg/App/tll_intranet/booking_searches

)

 

 

Users are only allowed to book the confocal system that they have received training on. If 
they wish to book other confocal system, they have to receive a separate training. 

 

Users are entitled to advance bookings of up to 2 weeks. They are advised to plan their 
experiments accordingly to avoid any disappointments. 

 

During Office Hours (Weekdays from 8.30am to 6pm)  

 

Each user is entitled to a 

MAXIMUM of 2 bookings

 per system per week. 

 

Users whom are trained on multiple systems are entitled to a maximum of 3 bookings 
per week but it has to be shared across the systems that they have received training 
on.  

 

Each booking must not exceed 3 hours.  

 

If users have utilized all their entitled bookings for the week, 

“24Hr Rule” 

can be 

applied where they can book the system in less than 24 hours in advance according to 
its availability. If extra slots are booked within the 24-hour period, a note of 

“24Hr 

Rule”

 should be made in the booking description.  

 

During Non - Office Hours (Weekdays after 6pm, Sat, Sun & Public holidays) 

 

If users require more slots in a particular week, they can book on weekdays during 
non-peak hours (

after 6pm

), on weekends (

Sat and Sun

) and on public holidays. 

 

If extra time is required, bookings can be extended out of peak hours

 (E.g. 3 

 7pm).

 

 

Bookings exceeding these limits are subjected to cancellation without prior warning.  

 

Simultaneous bookings of two or more different systems are not allowed. Multiple 
bookings for the same system on the same day during office hours is strictly prohibited. 

 

If users cannot attend a booked session for any reason, it is 

COMPULSORY

 that they 

cancel their booking through TLL intranet and email 

(

[email protected]rg.sg

)

 to announce the 

availability. If they are the last users for the day, they need to check if the system has 
been switched off completely. 

 

If users are swapping a session with another user, they must change the booking details 
accordingly. 

Summary of Contents for TCS SP8

Page 1: ...ng and System Access 5 3 1 Acknowledgements 6 4 Operation Procedures 6 4 1 Switching ON Protocol 7 4 2 Objective Lens 7 4 3 Software initiation 8 4 4 Turning on the lasers 8 4 5 Using Dye Assistant ta...

Page 2: ...Last Updated on Sep 2021 THIS PAGE IS INTENTIONALLY LEFT BLANK...

Page 3: ...ensure the lasers have sufficient time to heat up and cool down before running again When using the lasers to image NEVER look directly at the laser while imaging If a safety shutter is present make s...

Page 4: ...hen you notice that a bulb has reached between 2 000 2 500 hours or is not functioning properly 2 Training Use of confocal microscopy and the online booking system is authorized by facility staff only...

Page 5: ...week but it has to be shared across the systems that they have received training on Each booking must not exceed 3 hours If users have utilized all their entitled bookings for the week 24Hr Rule can b...

Page 6: ...Operation Procedures Every confocal system in TLL Bioimaging Facility has its specific instructional manual which are found in every confocal rooms Strictly adhere to the correct order of operation fo...

Page 7: ...re ON for a minimum of 2hrs before switching OFF as it requires sufficient time for warming up STED laser You may switch ON up to 100 Make sure the STED lasers are ON for at least 1hr before switching...

Page 8: ...4 4 Turning on the lasers 1 Under the Visible panel click on the button to open the laser panel 2 Click on the UV 405nm Visible Argon and SuperContVisible2 WLL bar to observe 405nm Argon and WLL respe...

Page 9: ...ector HyD or PMT you would like to image with your dyes HyD is more sensitive than PMT 4 The Dye assistant will give you a list of laser settings options to select from Select the settings which has t...

Page 10: ...our fluorophore Important Make sure that the emission band is at least 8 10nm away from your excitation line Choose an appropriate detector pseudocolour also known as a Look Up Table or LUT For PMT se...

Page 11: ...tional X Useful for live imaging unless you require fast imaging in which case you will need to adjust the phase correction Zoom is where you control the zoom parameters the arrows allow you to nudge...

Page 12: ...e background black level Pinhole the wider the pinhole aperture the thicker is the optical slice which means a blurrier but brighter image Make sure you also lower the laser power if you have to incre...

Page 13: ...3D Image Scroll down to the Z stack option in the Acquisition tab Select Live to see the specimen Use the focus knob on the USB control panel or point the cursor in the Z stack box panel and use the m...

Page 14: ...e any images which are older than 3 months Please make sure to do a backup Option 2 In TLL research cmn drive folder in your respective lab folders Option 3 In your own lab drive Option 4 In your pers...

Page 15: ...ethanol to remove any oil residues e In the event of logging off you may leave the lasers ON and exit LASX Otherwise Switch off all lasers within the software Diode Laser OFF 405nm White Light laser...

Page 16: ...s issue is a scanner issue you will not hear clicking as the program is shutting down and performing its pre shutdown WLL checks 2 Switch off the LSM switch 3 marked Scanner 3 Switch on the LSM 4 Open...

Page 17: ...If it says the HyD overloaded use the following steps 1 Turn off Live View 2 Ensure that the detection band is set at least 10nm away from any laser you are using 3 Set the HyD gain to 100 always Brig...

Page 18: ...Last Updated on Sep 2021 5 1 Troubleshooting Appendix Switches under computer terminal Power schematic diagram of the SP8...

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