Last Updated on Sep 2021
4.11 Switching OFF Protocol
Check through TLL microscopy resource booking if there is a user immediately after you. If
there is a user immediately after you, leave the system on and log off the PC. Otherwise switch
off the system.
(a)
Save your images through TLL common drive folder. Images saved in computer D/E
drives will be
deleted within a month without prior warning
.
(b)
Lower down the objective lens and carefully clean the objective lenses with Whatman
lens paper.
(c)
Make sure to switch your lens back to the 10x/0.3 objective lens.
(d)
Clean the sample holder with Kim wipes and 100% ethanol to remove any oil residues.
(e)
In the event of logging off, you may leave the lasers ON and exit LASX. Otherwise, Switch
off all lasers within the software.
•
Diode Laser
: “OFF” 405nm
•
White Light laser: “OFF”
•
Argon laser
: Slowly decrease the power to 0% and switch OFF only when it has run
for at least 2hrs. It needs a minimum of 2hrs for cooling before switching it ON.
•
STED laser
: Switch on the STED module
(6)
. Slowly decrease the power to 0% and
switch off only when it has run for at least an hour.
(f)
Exit LAS X software and switch off PC Microscope
(4)
.
(g)
Switch off STED laser module
(6)
if it was used.
(h)
Switch off Scanner power
(3),
Laser Power
(2)
and the Laser Key
(1)
.
(i)
Switch off the Metal Halide lamp if it was used
(5)
.
(j)
Fill in the logbook with the objective lens used and mention if ARGON/STED laser is still
ON/OFF.
