Last Updated on Sep 2021
power, until blue pixels just disappear. Decrease Offset until you have a dusting of green pixels
in the background where there are no cells.
When using the HyD detectors, gain is set at default 100 reduce it or the laser power to prevent
oversaturation. Activate gating when using the WLL to remove laser reflection or auto
fluorescence. Make sure to choose the correct reference line when using gating.
Click on QuickLUT button twice to switch from range indicator mode to normal colour view.
Click
Capture Image
or
Start
to capture an image.
4.9 How to do a Z stack for a 3D Image
•
Scroll down to the
Z-stack
option in the
Acquisition
tab.
•
Select
Live
to see the specimen.
•
Use the focus knob on the USB control panel or point the
cursor in the Z-stack box panel and use the mouse scroll
wheel to focus up and down (do not use the
microscope’s focus knob).
•
Move all the way to the top of your sample and click on
the
Begin
, turning it red, to mark the start
position.
•
Use the mouse scroll wheel to move to the bottom of your
sample and click on the
End
, turning it red to mark the end
position.
•
With
system optimized
selected the program will
determine the optimal settings for your Z-stack. You can
override these settings using the
Nr. of steps
or
z-step size
boxes. Beware that changing these will alter the z-
resolution you attain.
•
Click the
Start
button to begin the series.
